Kinesin Heavy Chain antibody | H2

Mouse anti Kinesin Heavy Chain

Product Type
Monoclonal Antibody
Clone
H2
Isotype
IgG2b
Specificity
Kinesin Heavy Chain

Product Code Applications Pack Size List Price Your Price Qty
OBT1670
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IB IP P WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Kinesin Heavy Chain antibody, clone H2 recognises the heavy chain of kinesin toward the N-terminus. The epitope is located in the region of amino acids 420-445.

Kinesins are microtubule associated motor proteins, responsible for converting nucleoside triphosphate released energy into mechanical energy. Kinesin-1 exists as a homodimer of 120-130 kDa chains, known as heavy chains. Associated with this dimer when purified from brain homogenates are light chains of 60-70 kDa. The light chains are involved in binding of kinesin to organelles and appear to have regulatory functions. They are not essential for motility generation.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals Expected from Sequence
Chicken Expected from Sequence
Invertebrates Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
Immunogen
Bovine brain kinesin.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunoblotting
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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References for Kinesin Heavy Chain antibody

  1. Pfister, K.K. et al. (1989) Monoclonal antibodies to kinesin heavy and light chains stain vesicle-like structures, but not microtubules, in cultured cells.
    J Cell Biol. 108 (4): 1453-63.
  2. Kamm, C. et al. (2004) The early onset dystonia protein torsinA interacts with kinesin light chain 1.
    J Biol Chem. 279 (19): 19882-92.

Synonyms
KIF5B
RRID
AB_2132375