GFAP antibody | 4A11

Mouse anti Bovine GFAP

Product Type
Monoclonal Antibody
Clone
4A11
Isotype
IgG2b
Specificity
GFAP

Product Code Applications Pack Size List Price Your Price Qty
MCA4684
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C P * R WB 0.25 mg loader
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loader
MCA4684GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C P * R WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Bovine GFAP antibody, clone 4A11 recognizes glial fibrillary acidic protein, or GFAP, a class III intermediate filament. During development of the central nervous system, GFAP distinguishes astrocytes from other glial cells. It is involved in various cellular functions, such as cell structure and movement, cell communication and the functioning of the blood-brain barrier. It also plays a role in mitosis by adjusting the filament network present in the cell.

Defects in GFAP are a cause of Alexander disease, a rare disorder of the central nervous system affecting mostly males. It is a progressive leukoencephalopathy resulting in mental and physical retardation, dementia, seizures and early death.

Mouse anti Bovine GFAP antibody, clone 4A11 may be used in conjunction with Mouse anti Bovine GFAP antibody, clone 1B4 (MCA4733) and Mouse anti Bovine GFAP antibody, clone 2E1 (MCA4734) for increased sensitivity when used in immunohistology.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Mouse
Rat
Sheep
Dog
Pig
Rabbit
Guinea Pig
Chicken
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Bovine spinal cord homogenate.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin 1
Radioimmunoassays
Western Blotting 1/500 1/10000
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Brain

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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loader
Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for GFAP antibody

  1. Pegram, C.N. et al. (1985) Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species.
    Neurochem Pathol. 3 (2): 119-38.
  2. McLendon, R.E. et al. (1986) The immunohistochemical application of three anti-GFAP monoclonal antibodies to formalin-fixed, paraffin-embedded, normal and neoplastic brain tissues.
    J Neuropathol Exp Neurol. 45 (6): 692-703.
  3. Zendedel, A. et al. (2016) Regulatory effect of triiodothyronine on brain myelination and astrogliosis after cuprizone-induced demyelination in mice.
    Metab Brain Dis. 31 (2): 425-33.

RRID
AB_10613491
UniProt
P14136
P47819
P03995
Q28115
Entrez Gene
Gfap
GFAP
Gfap
GFAP
GO Terms
GO:0005882 intermediate filament
GO:0005200 structural constituent of cytoskeleton
GO:0005737 cytoplasm
GO:0009611 response to wounding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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