CD80 antibody | IL-A159

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Anti Bovine CD80 Antibody, clone IL-A159 thumbnail image 1

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Anti Bovine CD80 Antibody, clone IL-A159 thumbnail image 3

Anti Bovine CD80 Antibody, clone IL-A159 thumbnail image 4

Anti Bovine CD80 Antibody, clone IL-A159 thumbnail image 5

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Anti Bovine CD80 Antibody, clone IL-A159 gallery image 1 — Figure A. FITC conjugated mouse anti bovine CD14 (MCA2678F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD14 (MCA2678F) and RPE conjugated mouse anti bovine CD80 (**MCA2436PE**). All experiments performed on red cell lysed bovine blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer.

Anti Bovine CD80 Antibody, clone IL-A159 gallery image 2 — Figure A. RPE conjugated mouse anti bovine CD80 (**MCA2436PE**) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti bovine CD80 (MCA2436PE) and FITC conjugated mouse anti bovine CD14 (MCA2678F). All experiments performed on red cell lysed bovine blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer.

Anti Bovine CD80 Antibody, clone IL-A159 gallery image 3 — **Published customer image:**
Mouse anti Bovine CD80 antibody, clone IL-A159 (**MCA2436GA**) used for the identification of CD80 expressing lymph node cells from naïve and contagious bovine pleuropneumonia infected infetcted cattle
**Image caption:**
Effect of free Mmm galactan on surface expression of CD40 ( A ) and CD80 ( B ) on bovine macrophages. Cells were incubated for 48 h with galactan (EPS) at 0.1, 1 and 10 μg/mL, or with LPS and medium only (C−) as positive and negative controls respectively. Cell-surface expression of CD40/CD80 was measured by flow cytometry and results expressed as mean (±SD) percentages of positive cells for a given marker (n = 3). Asterisks indicate statistically significant differences (p < 0.05) with the negative control.

From: Totté P, Puech C, Rodrigues V, Bertin C, Manso-Silvan L, Thiaucourt F.
Free exopolysaccharide from *Mycoplasma mycoides* subsp. *mycoides* possesses anti-inflammatory properties.
Vet Res. 2015 Oct 21;46(1):122.

Anti Bovine CD80 Antibody, clone IL-A159 gallery image 4 — Figure A. FITC conjugated mouse anti bovine CD62L (MCA1649F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD62L (MCA1649F) and RPE conjugated mouse anti bovine CD80 (**MCA2436PE**). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Anti Bovine CD80 Antibody, clone IL-A159 gallery image 5 — Figure A. RPE conjugated mouse anti bovine CD80 (**MCA2436PE**) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti bovine CD80 (**MCA2436PE**) and FITC conjugated mouse anti bovine CD62L (MCA1649F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Anti Bovine CD80 Antibody, clone IL-A159 gallery image 6 — Figure A. FITC conjugated mouse anti bovine CD14 (MCA2678F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD14 (MCA2678F) and RPE conjugated mouse anti bovine CD80 (**MCA2436PE**). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse anti Bovine CD80:FITC

Mouse anti Bovine CD80

Mouse anti Bovine CD80:RPE

Product Type: Monoclonal Antibody
Clone: IL-A159
Isotype: IgG1

Product Code	Applications	Pack Size
MCA2436F	F	0.1 mg
MCA2436GA	F	0.1 mg
MCA2436PE	F	100 Tests

Mouse anti Bovine CD80 antibody, clone IL-A159 recognizes the bovine CD80 cell surface antigen, expressed by dendritic cells, activated macrophages and activated B cells. CD80 plays a key role in co-stimulation of T cells during the primary immune response.

Product Details

Target Species

Bovine

Species Cross-Reactivity

Target Species	Cross Reactivity
Sheep

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Reconstitute with 1 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose

Carrier Free

Yes

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
Following reconstitution store at +4^oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: O46405 Related reagents
Regulatory: For research purposes only

Applications of CD80 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/10
Flow Cytometry	1/25	1/200
Flow Cytometry	Neat	1/50

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Bovine Dendritic Cell Growth Kit	PBP014KZZ	1 ml	FN
Bovine Dendritic Cell Growth Kit	PBP015KZZ	5 ml	FN
Bovine Dendritic Cell Growth Kit	PBP014KZZ	1 ml	FN
Bovine Dendritic Cell Growth Kit	PBP015KZZ	5 ml	FN

Application Based External Images

Flow Cytometry

Product Specific References

References for CD80 antibody

Langelaar, M.F. et al. (2005) Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70 interaction with different bovine antigen-presenting cells.
Scand J Immunol. 61 (3): 242-50.
Rhodes, S.G. et al. (2003) 1,25-dihydroxyvitamin D3 and development of tuberculosis in cattle.
Clin Diagn Lab Immunol. 10 (6): 1129-35.
Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
J Gen Virol. 84 (Pt 7): 1771-80.
Epardaud, M. et al. (2004) Enrichment for a CD26hi SIRP- subset in lymph dendritic cells from the upper aero-digestive tract.
J Leukoc Biol. 76 (3): 553-61.
Bonneau, M. et al. (2006) Migratory monocytes and granulocytes are major lymphatic carriers of Salmonella from tissue to draining lymph node.
J Leukoc Biol. 79: 268-76.
Hart, J. et al. (2011) Theileria annulata-transformed cell lines are efficient antigen-presenting cells for in vitro analysis of CD8 T cell responses to bovine herpesvirus-1.
Vet Res. 42: 119.
Ikebuchi, R. et al. (2013) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro.
Vet Res. 44: 59.
Totté P et al. (2015) Free exopolysaccharide from Mycoplasma mycoides subsp. mycoides possesses anti-inflammatory properties.
Vet Res. 46 (1): 122.
Corripio-miyar, Y. et al. (2017) 1,25-Dihydroxyvitamin D3 modulates the phenotype and function of Monocyte derived dendritic cells in cattle
BMC Veterinary Research. 13 (1) [Epub ahead of print].

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