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CD8 antibody | CC63

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Anti Bovine CD8 Antibody, clone CC63 thumbnail image 1 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 2 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 3 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 4 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 5 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 6 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 7 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 8 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 9 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 10 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 11
Anti Bovine CD8 Antibody, clone CC63 gallery image 1
Figure A. FITC conjugated Mouse anti Bovine CD21 (MCA1424F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Bovine CD21 (MCA1424F) and RPE conjugated Mouse anti Bovine CD8 (MCA837PE). All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Bovine CD8 Antibody, clone CC63 gallery image 2
Bovine lymph node stained with Mouse anti Bovine CD8 antibody, clone CC63(MCA837GA) 10x. Image courtesy of Dr. J.B. Silvan, Moredun Research Institute, Roslin, UK
Anti Bovine CD8 Antibody, clone CC63 gallery image 3
Bovine lymph node stained with Mouse anti Bovine CD8 antibody, clone CC63 (MCA837GA) 40x. Image courtesy of Dr. J.B. Silvan, Moredun Research Institute, Roslin, UK
Anti Bovine CD8 Antibody, clone CC63 gallery image 4
Published customer image:
Mouse anti Bovine CD8 antibody, clone CC63 used for the identification of CD8 expressing cells in the bovine nasopharynx by immunofluorescence.
Image caption:
Nasopharyngeal primary infection sites of vaccinated cattle are infiltrated by CD8+/CD3- (presumptive NK) cells at 24 hpi.
Multichannel immunofluorescent technique. A). FMDV VP1 (red) protein within cytokeratin+ cells (green) in epithelial crypt of the nasopharyngeal mucosa of non-vaccinated steer at 24 hpi (animal number 3). CD8+ (aqua)/ CD3+ (purple) double-positive CTLs are present in the subepithelial compartment amongst larger populations of CD8-/CD3+ T-lymphocytes. 20x magnification, scale bar 50μm. B) 40x magnification of region identified in (A), demonstrating consistent co-localization of CD8 (aqua) with CD3 (purple). Scale bars 25μm. B) Select channels of image shown in (B). CD3+ cells (purple) include single-positive (T-lymphocytes) or CD8+/CD3+ double-positive (CTLs). CD8 (aqua) is exclusively detected in combination with CD3 (purple). Scale bars 25 μm. D) FMDV VP1 (red) protein co-localize with cytokeratin (green) in a focal surface erosion within follicle-associated epithelium of nasopharyngeal mucosa of vaccinated steer at 24 hpi (animal number 11). A distinct population of cells defined as presumptive NK-cells based on a CD8+ (aqua)/CD3- (purple) phenotype is present in submucosal and epithelial compartments surrounding the focus of infection. A smaller population of CTLs (CD8+/CD3+) is present amongst abundant non-CTL T-lymphocytes (CD8-/CD3+). 20x magnification, scale bar 50μm. E) Higher magnification of region identified in showing CD8+/CD3- cells (aqua) representing presumptive NK cells in close proximity of FMDV infected focus. 40x magnification, scale bars 25 μm F) Individual channels of image shown in (E) demonstrating inconsistent co-localization of CD8 (aqua) and CD3 (purple). Scale bar 25 μm.

From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015)
Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle.
PLoS ONE 10(11): e0143666.
Anti Bovine CD8 Antibody, clone CC63 gallery image 5
Published customer image:
RPE conjugated Mouse anti Bovine CD8 antibody, clone CC63 (MCA837PE) used to detect cytotoxic T-cells in bovine peripheral blood by flow cytometry together with APC conjugated Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653) used for the identification of helper T-cells in the same samples.
Image caption:
Insert image caption

From: Sun F, Cao Y, Cai C, Li S, Yu C, Yao J (2016)
Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine.
PLoS ONE 11(8): e0160659.
Anti Bovine CD8 Antibody, clone CC63 gallery image 6
Figure A. FITC conjugated mouse anti bovine CD2 (MCA833F) and RPE conjugated mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated mouse anti bovine CD2 (MCA833F) and RPE conjugated mouse anti bovine CD8 (MCA837PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD8 Antibody, clone CC63 gallery image 7
Figure A. RPE conjugated mouse anti bovine CD8 (MCA837PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti bovine CD4 (MCA837PE) and FITC conjugated mouse anti bovine CD2 (MCA833F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD8 Antibody, clone CC63 gallery image 8
Figure A. A647 conjugated mouse anti bovine CD26 (MCA1652A647) and FITC conjugated mouse IgG2a isotype control (MCA929F). Figure B. A647 conjugated mouse anti bovine CD26 (MCA1652A647) and FITC conjugated mouse anti bovine CD8 (MCA837F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD8 Antibody, clone CC63 gallery image 9
Figure A. FITC conjugated mouse anti bovine CD8 (MCA837F) and A647 conjugated mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated mouse anti bovine CD8 (MCA837F) and A647 conjugated mouse anti bovine CD26 (MCA1652A647). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD8 Antibody, clone CC63 gallery image 10
Published customer image:
Mouse anti Bovine CD8 antibody, clone CC63 (MCA837GA) used for the identification of CD8 expressing lymphocytes in bovine tissue sections by immunohistochemistry on formalin fixed, paraffin sections.
Image caption:
Immunohistochemical staining of CD8 + T cells of the Bovine mammary tissue using paraffin-embedded sections (A–C): (A) The intermediate immunoreactivity of CD8 + T lymphocytes (arrow) in the interalveolar connective tissue with the monoclonal antibody specific for CD8+ T cells in Bovine mammary tissue infected with BPIV-3. (B) The low immunoreactivity of T lymphocytes (arrow) in the interalveolar connective tissue with the monoclonal antibody specific for CD8+ T cells in Bovine mammary tissue infected with BoHV-1. (C) The high reactivity distributional of T lymphocytes in the interalveolar connective tissue with the monoclonal antibody specific for CD8+ T cells (arrow) in Bovine mammary tissue infected with BoHV-1 and BPIV-3. IHC. Bar, 20μm.

From: Çomakli S, Özdemir S. Comparative Evaluation of the Immune Responses in Cattle Mammary Tissues Naturally Infected with Bovine Parainfluenza Virus Type 3 and Bovine Alphaherpesvirus-1. Pathogens. 2019;8(1). pii: E26.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Bovine CD8 Antibody, clone CC63 gallery image 11
Published customer image:
FITC conjugated Mouse anti Bovine CD8 antibody, clone CC63 (MCA837F) used for the evaluation of CD8 expression on ovine peritoneal cells by flow cytometry.
Image caption:
Percentage of cell populations in the peritoneal fluid during the early stages of infection. The specific cell populations are shown as follows: (a) TCD4; (b) TCD8; (c) WC1+γδ; (d) CD14; (e) MHCII; (f) CD83. Cell subsets of uninfected (Neg. Ctl) and infected sheep (Group 1) are shown in columns. Mann-Whitney U-test was used to compare data from Groups 1 and 3. Values represent the mean ± standard deviation (SD). Asterisks indicate different levels of significance between groups: *P ≤ 0.05, **P ≤ 0.01

From: Pérez-Caballero R, Javier Martínez-Moreno F, Zafra R, Molina-Hernández V, Pacheco IL, Teresa Ruiz-Campillo M, Escamilla A, Pérez J, Martnez-Moreno Á, Buffoni L.
Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
Parasit Vectors. 2018 11(1):640.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Bovine CD8:Alexa Fluor® 647

Mouse anti Bovine CD8:FITC

Mouse anti Bovine CD8

Mouse anti Bovine CD8:RPE

Product Type
Monoclonal Antibody
Clone
CC63
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA837A647
Datasheet
F
MSDS
100 Tests/1ml loader

MCA837F
Datasheet
F
MSDS
100 Tests loader

MCA837GA
Datasheet
C F IF IP P
MSDS
0.1 mg loader

MCA837PE
Datasheet
F
MSDS
100 Tests loader

Mouse anti Bovine CD8 antibody, clone CC63 reacts with the bovine CD8 antigen expressed by a subset of T lymphocytes. The antibody precipitates molecules of ~34 kDa and ~38 kDa under reducing conditions. Clone CC63 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Gutierrez et al. 1999).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

This product should be stored undiluted.

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P31783
Entrez Gene
CD8A
GO Terms
GO:0016021 integral to membrane
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat
Flow Cytometry 1/50
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F F 0.1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 647 MCA929A647 F 100 Tests/1ml loader
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests loader

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD8 antibody

  1. MacHugh, N.D. & Sopp P (1991) Individual antigens of cattle. Bovine CD8 (BoCD8).
    Vet Immunol Immunopathol. 27 (1-3): 65-9.
  2. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
    Vet Immunol Immunopathol. 71 (3-4): 321-34.
  3. Twizere, J.C. et al. (2000) Discordance between bovine leukemia virus tax immortalization in vitro and oncogenicity in vivo.
    J Virol. 74 (21): 9895-902.
  4. Winkler, M.T. et al. (1999) Bovine herpesvirus 1 can infect CD4(+) T lymphocytes and induce programmed cell death during acute infection of cattle.
    J Virol. 73 (10): 8657-68.
  5. Winkler, M.T. et al. (2000) Persistence and reactivation of bovine herpesvirus 1 in the tonsils of latently infected calves.
    J Virol. 74 (11): 5337-46.
  6. Sidders, B. et al. (2008) Screening of highly expressed mycobacterial genes identifies Rv3615c as a useful differential diagnostic antigen for the Mycobacterium tuberculosis complex.
    Infect Immun. 76: 3932-9.
  7. Sanchez, J. et al. (2011) Microscopical and immunological features of tuberculoid granulomata and cavitary pulmonary tuberculosis in naturally infected goats.
    J Comp Pathol. 145 (2-3): 107-17.
  8. La Manna, M.P. et al. (2011) Expansion of intracellular IFN-γ positive lymphocytes during Mycoplasma agalactiae infection in sheep.
    Res Vet Sci. 91 (3): e64-7.
  9. Fulton, B.E. Jr. et al. (2006) Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node.
    J Virol. 80: 7873-84.
  10. Harris, J. et al. (2002) Expression of caveolin by bovine lymphocytes and antigen-presenting cells.
    Immunology. 105: 190-5.
  11. Liebana, E. et al. (2007) Distribution and activation of T-lymphocyte subsets in tuberculous bovine lymph-node granulomas.
    Vet Pathol. 44: 366-72.
  12. Lynch, E.M. et al. (2010) Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves.
    BMC Vet Res. 6: 39.
  13. Lacroux, C. et al. (2012) Prionemia and leukocyte-platelet-associated infectivity in sheep transmissible spongiform encephalopathy models.
    J Virol. 86 (4): 2056-66.
  14. Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.
    Vet Res. 41: 14.
  15. Toman, M. et al. (2003) Immunological characteristics of ca􀄴le with Mycobacterium avium subsp. paratuberculosis infection
    Vet. Med. – Czech, 48, 2003: 147–54.
  16. Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.
    Int J Parasitol. 40: 865-75.
  17. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
    Theriogenology. 82 (3): 403-10.
  18. Romero-Palomo F et al. (2015) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
    Transbound Emerg Dis. Aug 25. [Epub ahead of print]
  19. Stenfeldt, C. et al. (2015) Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle.
    PLoS One. 10 (11): e0143666.
  20. Leite FL et al. (2015) ZAP-70, CTLA-4 and proximal T cell receptor signaling in cows infected with Mycobacterium avium subsp. paratuberculosis.
    Vet Immunol Immunopathol. 167 (1-2): 15-21.
  21. Silva, A.P. et al. (2015) Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (&Detla;abcBA) Protects against Wild Type Brucella ovis in Rams.
    PLoS One. 10 (8): e0136865.
  22. Schmidt, N. et al. (2018) Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoids.
    Vet Res. 49 (1): 28.
  23. Benedictus, L. et al. (2019) Immunization of young heifers with staphylococcal immune evasion proteins before natural exposure to Staphylococcus aureus induces a humoral immune response in serum and milk.
    BMC Vet Res. 15 (1): 15.
  24. Pérez-caballero, R. et al. (2018) Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
    Parasit Vectors. 11 (1): 640.
  25. Bloomer, S.A. et al. (2019) Aging results in accumulation of M1 and M2 hepatic macrophages and a differential response to gadolinium chloride.
    Histochem Cell Biol. Nov 06 [Epub ahead of print].
  26. Foulon, E. & Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
    J Immunol Methods. 339 (1): 1-10.
  27. Brodzki, P. et al. (2019) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
    Anim Sci J. Nov 06 [Epub ahead of print].
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