CD8 antibody | CC63
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Mouse anti Bovine CD8
- Product Type
- Monoclonal Antibody
|Mouse anti Bovine CD8 antibody, clone CC63 reacts with the bovine CD8 antigen expressed by a subset of T lymphocytes. The antibody precipitates molecules of ~34 kDa and ~38 kDa under reducing conditions. Clone CC63 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Gutierrez et al. 1999).|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Sheep Goat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD8 antibody
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Vet Immunol Immunopathol. 27 (1-3): 65-9.
Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
Vet Immunol Immunopathol. 71 (3-4): 321-34.
Winkler, M.T. et al. (1999) Bovine herpesvirus 1 can infect CD4(+) T lymphocytes and induce programmed cell death during acute infection of cattle.
J Virol. 73 (10): 8657-68.
Winkler, M.T. et al. (2000) Persistence and reactivation of bovine herpesvirus 1 in the tonsils of latently infected calves.
J Virol. 74 (11): 5337-46.
Twizere, J.C. et al. (2000) Discordance between bovine leukemia virus tax immortalization in vitro and oncogenicity in vivo.
J Virol. 74 (21): 9895-902.
Harris, J. et al. (2002) Expression of caveolin by bovine lymphocytes and antigen-presenting cells.
Immunology. 105: 190-5.
Toman, M. et al. (2003) Immunological characteristics of cale with Mycobacterium avium subsp. paratuberculosis infection
Vet. Med. – Czech, 48, 2003: 147–54.
Vordermeier, H.M. et al. (2004) Cellular immune responses induced in cattle by heterologous prime-boost vaccination using recombinant viruses and bacille Calmette-Guérin.
Immunology. 112: 461-70.
View The Latest Product References
Vitale, F. et al. (2006) ESAT-6 peptide recognition by bovine CD8+ lymphocytes of naturally infected cows in herds from southern Italy.
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Fulton, B.E. Jr. et al. (2006) Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node.
J Virol. 80: 7873-84.
Liebana, E. et al. (2007) Distribution and activation of T-lymphocyte subsets in tuberculous bovine lymph-node granulomas.
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Foulon, E. & Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
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Sidders, B. et al. (2008) Screening of highly expressed mycobacterial genes identifies Rv3615c as a useful differential diagnostic antigen for the Mycobacterium tuberculosis complex.
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Lynch, E.M. et al. (2010) Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves.
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Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.
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Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.
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La Manna, M.P. et al. (2011) Expansion of intracellular IFN-γ positive lymphocytes during Mycoplasma agalactiae infection in sheep.
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Sanchez, J. et al. (2011) Microscopical and immunological features of tuberculoid granulomata and cavitary pulmonary tuberculosis in naturally infected goats.
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Lacroux, C. et al. (2012) Prionemia and leukocyte-platelet-associated infectivity in sheep transmissible spongiform encephalopathy models.
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Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
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Silva, A.P. et al. (2015) Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (&Detla;abcBA) Protects against Wild Type Brucella ovis in Rams.
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Leite FL et al. (2015) ZAP-70, CTLA-4 and proximal T cell receptor signaling in cows infected with Mycobacterium avium subsp. paratuberculosis.
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PLoS One. 10 (11): e0143666.
Romero-Palomo, F. et al. (2017) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
Transbound Emerg Dis. 64 (2): 574-84.
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Pérez-caballero, R. et al. (2018) Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
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Nakajima, N. et al. (2019) Effects of direct exposure to cold weather under grazing in winter on the physiological, immunological, and behavioral conditions of Japanese Black beef cattle in central Japan.
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de Araújo, F.F. et al. (2019) Distinct immune response profile during Rhipicephalus (Boophilus) microplus. infestations of guzerat dairy herd according to the maternal lineage ancestry (mitochondrial DNA).
Vet Parasitol. 273: 36-44.
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Brodzki, P. et al. (2020) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
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Bloomer, S.A. et al. (2020) Aging results in accumulation of M1 and M2 hepatic macrophages and a differential response to gadolinium chloride.
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Elsayed, M.S.A.E. et al. (2022) Real-time PCR using atpE, conventional PCR targeting different regions of difference, and flow cytometry for confirmation of Mycobacterium bovis. in buffaloes and cattle from the Delta area of Egypt.
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