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CD8 antibody | CC63

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Anti Bovine CD8 Antibody, clone CC63 thumbnail image 1 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 2 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 3 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 4 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 5 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 6 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 7 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 8 Anti Bovine CD8 Antibody, clone CC63 thumbnail image 9
Anti Bovine CD8 Antibody, clone CC63 gallery image 1
Figure A. FITC conjugated Mouse anti Bovine CD21 (MCA1424F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Bovine CD21 (MCA1424F) and RPE conjugated Mouse anti Bovine CD8 (MCA837PE). All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Bovine CD8 Antibody, clone CC63 gallery image 2
Bovine lymph node stained with Mouse anti Bovine CD8 antibody, clone CC63(MCA837GA) 10x. Image courtesy of Dr. J.B. Silvan, Moredun Research Institute, Roslin, UK
Anti Bovine CD8 Antibody, clone CC63 gallery image 3
Bovine lymph node stained with Mouse anti Bovine CD8 antibody, clone CC63 (MCA837GA) 40x. Image courtesy of Dr. J.B. Silvan, Moredun Research Institute, Roslin, UK
Anti Bovine CD8 Antibody, clone CC63 gallery image 4
Published customer image:
Mouse anti Bovine CD8 antibody, clone CC63 used for the identification of CD8 expressing cells in the bovine nasopharynx by immunofluorescence.
Image caption:
Nasopharyngeal primary infection sites of vaccinated cattle are infiltrated by CD8+/CD3- (presumptive NK) cells at 24 hpi.
Multichannel immunofluorescent technique. A). FMDV VP1 (red) protein within cytokeratin+ cells (green) in epithelial crypt of the nasopharyngeal mucosa of non-vaccinated steer at 24 hpi (animal number 3). CD8+ (aqua)/ CD3+ (purple) double-positive CTLs are present in the subepithelial compartment amongst larger populations of CD8-/CD3+ T-lymphocytes. 20x magnification, scale bar 50μm. B) 40x magnification of region identified in (A), demonstrating consistent co-localization of CD8 (aqua) with CD3 (purple). Scale bars 25μm. B) Select channels of image shown in (B). CD3+ cells (purple) include single-positive (T-lymphocytes) or CD8+/CD3+ double-positive (CTLs). CD8 (aqua) is exclusively detected in combination with CD3 (purple). Scale bars 25 μm. D) FMDV VP1 (red) protein co-localize with cytokeratin (green) in a focal surface erosion within follicle-associated epithelium of nasopharyngeal mucosa of vaccinated steer at 24 hpi (animal number 11). A distinct population of cells defined as presumptive NK-cells based on a CD8+ (aqua)/CD3- (purple) phenotype is present in submucosal and epithelial compartments surrounding the focus of infection. A smaller population of CTLs (CD8+/CD3+) is present amongst abundant non-CTL T-lymphocytes (CD8-/CD3+). 20x magnification, scale bar 50μm. E) Higher magnification of region identified in showing CD8+/CD3- cells (aqua) representing presumptive NK cells in close proximity of FMDV infected focus. 40x magnification, scale bars 25 μm F) Individual channels of image shown in (E) demonstrating inconsistent co-localization of CD8 (aqua) and CD3 (purple). Scale bar 25 μm.

From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015)
Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle.
PLoS ONE 10(11): e0143666.
Anti Bovine CD8 Antibody, clone CC63 gallery image 5
Published customer image:
RPE conjugated Mouse anti Bovine CD8 antibody, clone CC63 (MCA837PE) used to detect cytotoxic T-cells in bovine peripheral blood by flow cytometry together with APC conjugated Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653) used for the identification of helper T-cells in the same samples.
Image caption:
Insert image caption

From: Sun F, Cao Y, Cai C, Li S, Yu C, Yao J (2016)
Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine.
PLoS ONE 11(8): e0160659.
Anti Bovine CD8 Antibody, clone CC63 gallery image 6
Figure A. FITC conjugated mouse anti bovine CD2 (MCA833F) and RPE conjugated mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated mouse anti bovine CD2 (MCA833F) and RPE conjugated mouse anti bovine CD8 (MCA837PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD8 Antibody, clone CC63 gallery image 7
Figure A. RPE conjugated mouse anti bovine CD8 (MCA837PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti bovine CD4 (MCA837PE) and FITC conjugated mouse anti bovine CD2 (MCA833F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD8 Antibody, clone CC63 gallery image 8
Figure A. A647 conjugated mouse anti bovine CD26 (MCA1652A647) and FITC conjugated mouse IgG2a isotype control (MCA929F). Figure B. A647 conjugated mouse anti bovine CD26 (MCA1652A647) and FITC conjugated mouse anti bovine CD8 (MCA837F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD8 Antibody, clone CC63 gallery image 9
Figure A. FITC conjugated mouse anti bovine CD8 (MCA837F) and A647 conjugated mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated mouse anti bovine CD8 (MCA837F) and A647 conjugated mouse anti bovine CD26 (MCA1652A647). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse anti Bovine CD8:Alexa Fluor® 647

Mouse anti Bovine CD8:FITC

Mouse anti Bovine CD8

Mouse anti Bovine CD8:RPE

Product Type
Monoclonal Antibody
Clone
CC63
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA837A647 F 100 Tests/1ml
MCA837F F 100 Tests
MCA837GA C F IF IP P 0.1 mg
MCA837PE F 100 Tests
Mouse anti Bovine CD8 antibody, clone CC63 reacts with the bovine CD8 antigen expressed by a subset of T lymphocytes. The antibody precipitates molecules of ~34 kDa and ~38 kDa under reducing conditions. Clone CC63 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Gutierrez et al. 1999).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

This product should be stored undiluted.

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P31783 Related reagents
Entrez Gene
CD8A Related reagents
GO Terms
GO:0016021 integral to membrane
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat
Flow Cytometry 1/50
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 647 MCA929A647 100 Tests/1ml F
Mouse IgG2a Negative Control:FITC MCA929F 100 Tests F
Mouse IgG2a Negative Control MCA929 100 Tests F
Mouse IgG2a Negative Control:RPE MCA929PE 100 Tests F

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD8 antibody

  1. MacHugh, N.D. & Sopp P (1991) Individual antigens of cattle. Bovine CD8 (BoCD8).
    Vet Immunol Immunopathol. 27 (1-3): 65-9.
  2. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
    Vet Immunol Immunopathol. 71 (3-4): 321-34.
  3. Twizere, J.C. et al. (2000) Discordance between bovine leukemia virus tax immortalization in vitro and oncogenicity in vivo.
    J Virol. 74 (21): 9895-902.
  4. Winkler, M.T. et al. (1999) Bovine herpesvirus 1 can infect CD4(+) T lymphocytes and induce programmed cell death during acute infection of cattle.
    J Virol. 73 (10): 8657-68.
  5. Winkler, M.T. et al. (2000) Persistence and reactivation of bovine herpesvirus 1 in the tonsils of latently infected calves.
    J Virol. 74 (11): 5337-46.
  6. Sidders, B. et al. (2008) Screening of highly expressed mycobacterial genes identifies Rv3615c as a useful differential diagnostic antigen for the Mycobacterium tuberculosis complex.
    Infect Immun. 76: 3932-9.
  7. Sanchez, J. et al. (2011) Microscopical and immunological features of tuberculoid granulomata and cavitary pulmonary tuberculosis in naturally infected goats.
    J Comp Pathol. 145 (2-3): 107-17.
  8. La Manna, M.P. et al. (2011) Expansion of intracellular IFN-γ positive lymphocytes during Mycoplasma agalactiae infection in sheep.
    Res Vet Sci. 91 (3): e64-7.
  9. Fulton, B.E. Jr. et al. (2006) Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node.
    J Virol. 80: 7873-84.
  10. Harris, J. et al. (2002) Expression of caveolin by bovine lymphocytes and antigen-presenting cells.
    Immunology. 105: 190-5.
  11. Liebana, E. et al. (2007) Distribution and activation of T-lymphocyte subsets in tuberculous bovine lymph-node granulomas.
    Vet Pathol. 44: 366-72.
  12. Lynch, E.M. et al. (2010) Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves.
    BMC Vet Res. 6: 39.
  13. Lacroux, C. et al. (2012) Prionemia and leukocyte-platelet-associated infectivity in sheep transmissible spongiform encephalopathy models.
    J Virol. 86 (4): 2056-66.
  14. Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.
    Vet Res. 41: 14.
  15. Toman, M. et al. (2003) Immunological characteristics of ca􀄴le with Mycobacterium avium subsp. paratuberculosis infection
    Vet. Med. – Czech, 48, 2003: 147–54
  16. Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus0 cattle.
    Int J Parasitol. 40: 865-75.
  17. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
    Theriogenology. 82 (3): 403-10.
  18. Romero-Palomo F et al. (2015) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
    Transbound Emerg Dis. Aug 25. [Epub ahead of print]
  19. Stenfeldt, C. et al. (2015) Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle.
    PLoS One. 10 (11): e0143666.
  20. Leite FL et al. (2015) ZAP-70, CTLA-4 and proximal T cell receptor signaling in cows infected with Mycobacterium avium subsp. paratuberculosis.
    Vet Immunol Immunopathol. 167 (1-2): 15-21.
  21. Silva, A.P. et al. (2015) Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (&Detla;abcBA) Protects against Wild Type Brucella ovis in Rams.
    PLoS One. 10 (8): e0136865.
  22. Schmidt, N. et al. (2018) Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoids.
    Vet Res. 49 (1): 28.
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