CD8 antibody | CC63
RPE conjugated Mouse anti Bovine CD8 antibody, clone CC63 (MCA837PE) used to detect cytotoxic T-cells in bovine peripheral blood by flow cytometry together with APC conjugated Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653) used for the identification of helper T-cells in the same samples.
Image caption:
Insert image caption
From: Sun F, Cao Y, Cai C, Li S, Yu C, Yao J (2016)
Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine.
PLoS ONE 11(8): e0160659.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine CD8 antibody, clone CC63 (MCA837GA) used for the identification of CD8 expressing lymphocytes in bovine tissue sections by immunohistochemistry on formalin fixed, paraffin sections.
Image caption:
Immunohistochemical staining of CD8 + T cells of the Bovine mammary tissue using paraffin-embedded sections (A–C): (A) The intermediate immunoreactivity of CD8 + T lymphocytes (arrow) in the interalveolar connective tissue with the monoclonal antibody specific for CD8+ T cells in Bovine mammary tissue infected with BPIV-3. (B) The low immunoreactivity of T lymphocytes (arrow) in the interalveolar connective tissue with the monoclonal antibody specific for CD8+ T cells in Bovine mammary tissue infected with BoHV-1. (C) The high reactivity distributional of T lymphocytes in the interalveolar connective tissue with the monoclonal antibody specific for CD8+ T cells (arrow) in Bovine mammary tissue infected with BoHV-1 and BPIV-3. IHC. Bar, 20μm.
From: Çomakli S, Özdemir S. Comparative Evaluation of the Immune Responses in Cattle Mammary Tissues Naturally Infected with Bovine Parainfluenza Virus Type 3 and Bovine Alphaherpesvirus-1. Pathogens. 2019;8(1). pii: E26.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Bovine CD8 antibody, clone CC63 (MCA837F) used for the evaluation of CD8 expression on ovine peritoneal cells by flow cytometry.
Image caption:
Percentage of cell populations in the peritoneal fluid during the early stages of infection. The specific cell populations are shown as follows: (a) TCD4; (b) TCD8; (c) WC1+γδ; (d) CD14; (e) MHCII; (f) CD83. Cell subsets of uninfected (Neg. Ctl) and infected sheep (Group 1) are shown in columns. Mann-Whitney U-test was used to compare data from Groups 1 and 3. Values represent the mean ± standard deviation (SD). Asterisks indicate different levels of significance between groups: *P ≤ 0.05, **P ≤ 0.01
From: Pérez-Caballero R, Javier Martínez-Moreno F, Zafra R, Molina-Hernández V, Pacheco IL, Teresa Ruiz-Campillo M, Escamilla A, Pérez J, Martnez-Moreno Á, Buffoni L.
Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
Parasit Vectors. 2018 11(1):640.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Bovine CD8 antibody, clone CC63 used for the identification of CD8 expressing cells in the bovine nasopharynx by immunofluorescence.
Image caption:
Nasopharyngeal primary infection sites of vaccinated cattle are infiltrated by CD8+/CD3- (presumptive NK) cells at 24 hpi.
Multichannel immunofluorescent technique. A). FMDV VP1 (red) protein within cytokeratin+ cells (green) in epithelial crypt of the nasopharyngeal mucosa of non-vaccinated steer at 24 hpi (animal number 3). CD8+ (aqua)/ CD3+ (purple) double-positive CTLs are present in the subepithelial compartment amongst larger populations of CD8-/CD3+ T-lymphocytes. 20x magnification, scale bar 50μm. B) 40x magnification of region identified in (A), demonstrating consistent co-localization of CD8 (aqua) with CD3 (purple). Scale bars 25μm. B) Select channels of image shown in (B). CD3+ cells (purple) include single-positive (T-lymphocytes) or CD8+/CD3+ double-positive (CTLs). CD8 (aqua) is exclusively detected in combination with CD3 (purple). Scale bars 25 μm. D) FMDV VP1 (red) protein co-localize with cytokeratin (green) in a focal surface erosion within follicle-associated epithelium of nasopharyngeal mucosa of vaccinated steer at 24 hpi (animal number 11). A distinct population of cells defined as presumptive NK-cells based on a CD8+ (aqua)/CD3- (purple) phenotype is present in submucosal and epithelial compartments surrounding the focus of infection. A smaller population of CTLs (CD8+/CD3+) is present amongst abundant non-CTL T-lymphocytes (CD8-/CD3+). 20x magnification, scale bar 50μm. E) Higher magnification of region identified in showing CD8+/CD3- cells (aqua) representing presumptive NK cells in close proximity of FMDV infected focus. 40x magnification, scale bars 25 μm F) Individual channels of image shown in (E) demonstrating inconsistent co-localization of CD8 (aqua) and CD3 (purple). Scale bar 25 μm.
From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015)
Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle.
PLoS ONE 10(11): e0143666.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine CD8 antibody, clone CC63 (MCA837GA) used to evaluate CD8 expression in bovine granulomatous lesions by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunolabelling for CD8+ cells (ABC IHC stain, DAB brown chromogen). (A) Comparison of CD8+ cell distribution in different granuloma stages of BCG vaccinated (Vac) and non-vaccinated (Con) calves. (B) Stage I granuloma from non-vaccinated calf showing very few stained CD8+ cells (arrows). (C) Stage I granuloma from BCG vaccinated calf showing stained CD8+ cells (arrows). (D) Stage III granuloma from non-vaccinated calf showing few stained CD8+ cells (arrows). (E) Stage III granuloma from vaccinated calf showing abundance of CD8+ cells (arrows). Bar equals 50μm in (B,C) and 100μm in (D,E).
From: Sirak A, Tulu B, Bayissa B, Gumi B, Berg S, Salguero FJ, Ameni G; ETHICOBOTS Consortium.
Cellular and Cytokine Responses in Lymph Node Granulomas of Bacillus Calmette Guérin (BCG)-Vaccinated and Non-vaccinated Cross-Breed Calves Naturally Infected With Mycobacterium bovis.
Front Vet Sci. 2021 Sep 16;8:698800.
doi: 10.3389/fvets.2021.698800.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Alexa Fluor 647 conjugated Mouse anti Bovine CD8 antibody, clone CC63 (MCA837A647) used to identify T cell populations in bovime blood samples by flow cytometry.
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Changes in T and B lymphocytes among PBMCs in Holstein and Jersey steers subjected to heat stress. Flow cytometry analysis to identify lymphocytes subset population. Heat stress caused reduction in lymphocyte populations in the PBMCs of both Holstein and Jersey steers. The lymphocytes comprised CD4+ T cells. MT indicates moderate THI condition and HT indicates high THI condition. * = P <0.05, ** = P <0.01
From: Park DS, Gu BH, Park YJ, Joo SS, Lee SS, Kim SH, Kim ET, Kim DH, Lee SS, Lee SJ, Kim BW, Kim M.
Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
Cell Stress Chaperones. 2021 Jul;26(4):705-20.
doi: 10.1007/s12192-021-01216-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
R-Phycoerythrin conjugated Mouse anti Bovine CD8 antibody, clone CC63 (MCA837PE) used to evaluate CD8 expression on bovine lymphocytes by flow cytometry.
Image caption:
Representative dot plots, from bovine PBMCs, used for selecting the lymphocyte region based on side sideward scatter (SSC) on the y-axis and forward side scatter (FSC) on the x-axis. Then, we selected the CD8 region based on fluorescence anti-CD8 stain on the y-axis and CD4 region based on fluorescence anti-CD4 stain on the x-axis. PBMCs incubated for 18 h with iFMDV are shown.
From: Bidart J, Kornuta C, Gammella M, Gnazzo V, Soria I, Langellotti C, Mongini C, Galarza R, Calvinho L, Lupi G, Quattrocchi V, Marcipar I, Zamorano P.
A New Cage-Like Particle Adjuvant Enhances Protection of Foot-and-Mouth Disease Vaccine.
Front Vet Sci. 2020 Jul 31;7:396.
doi: 10.3389/fvets.2020.00396.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F C P IF ResetMouse anti Bovine CD8
- Product Type
- Monoclonal Antibody
- Clone
- CC63
- Isotype
- IgG2a
- Specificity
- CD8
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Antibody Quality and 
Unrivaled 
| Mouse anti Bovine CD8 antibody, clone CC63 reacts with the bovine CD8 antigen expressed by a subset of T lymphocytes. The antibody precipitates molecules of ~34 kDa and ~38 kDa under reducing conditions. Clone CC63 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Gutierrez et al. 1999). |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | |
| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin | |
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| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| Description | Mouse IgG2a Negative Control | ||||||
References for CD8 antibody
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Vitale, F. et al. (2006) ESAT-6 peptide recognition by bovine CD8+ lymphocytes of naturally infected cows in herds from southern Italy.
Clin Vaccine Immunol. 13: 530-3. -
Vordermeier, H.M. et al. (2004) Cellular immune responses induced in cattle by heterologous prime-boost vaccination using recombinant viruses and bacille Calmette-Guérin.
Immunology. 112: 461-70. -
MacHugh, N.D. & Sopp P (1991) Individual antigens of cattle. Bovine CD8 (BoCD8).
Vet Immunol Immunopathol. 27 (1-3): 65-9. -
Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
Vet Immunol Immunopathol. 71 (3-4): 321-34. -
Twizere, J.C. et al. (2000) Discordance between bovine leukemia virus tax immortalization in vitro and oncogenicity in vivo.
J Virol. 74 (21): 9895-902. -
Winkler, M.T. et al. (1999) Bovine herpesvirus 1 can infect CD4(+) T lymphocytes and induce programmed cell death during acute infection of cattle.
J Virol. 73 (10): 8657-68. -
Winkler, M.T. et al. (2000) Persistence and reactivation of bovine herpesvirus 1 in the tonsils of latently infected calves.
J Virol. 74 (11): 5337-46. -
Sidders, B. et al. (2008) Screening of highly expressed mycobacterial genes identifies Rv3615c as a useful differential diagnostic antigen for the Mycobacterium tuberculosis complex.
Infect Immun. 76: 3932-9.
View The Latest Product References
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Sanchez, J. et al. (2011) Microscopical and immunological features of tuberculoid granulomata and cavitary pulmonary tuberculosis in naturally infected goats.
J Comp Pathol. 145 (2-3): 107-17. -
La Manna, M.P. et al. (2011) Expansion of intracellular IFN-γ positive lymphocytes during Mycoplasma agalactiae infection in sheep.
Res Vet Sci. 91 (3): e64-7. -
Fulton, B.E. Jr. et al. (2006) Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node.
J Virol. 80: 7873-84. -
Harris, J. et al. (2002) Expression of caveolin by bovine lymphocytes and antigen-presenting cells.
Immunology. 105: 190-5. -
Liebana, E. et al. (2007) Distribution and activation of T-lymphocyte subsets in tuberculous bovine lymph-node granulomas.
Vet Pathol. 44: 366-72. -
Lynch, E.M. et al. (2010) Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves.
BMC Vet Res. 6: 39. -
Lacroux, C. et al. (2012) Prionemia and leukocyte-platelet-associated infectivity in sheep transmissible spongiform encephalopathy models.
J Virol. 86 (4): 2056-66. -
Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.
Vet Res. 41: 14. -
Toman, M. et al. (2003) Immunological characteristics of cale with Mycobacterium avium subsp. paratuberculosis infection
Vet. Med. – Czech, 48, 2003: 147–54. -
Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.
Int J Parasitol. 40: 865-75. -
Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
Theriogenology. 82 (3): 403-10. -
Romero-Palomo F et al. (2015) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
Transbound Emerg Dis. Aug 25. [Epub ahead of print] -
Stenfeldt, C. et al. (2015) Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle.
PLoS One. 10 (11): e0143666. -
Leite FL et al. (2015) ZAP-70, CTLA-4 and proximal T cell receptor signaling in cows infected with Mycobacterium avium subsp. paratuberculosis.
Vet Immunol Immunopathol. 167 (1-2): 15-21. -
Silva, A.P. et al. (2015) Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (&Detla;abcBA) Protects against Wild Type Brucella ovis in Rams.
PLoS One. 10 (8): e0136865. -
Schmidt, N. et al. (2018) Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoids.
Vet Res. 49 (1): 28. -
Benedictus, L. et al. (2019) Immunization of young heifers with staphylococcal immune evasion proteins before natural exposure to Staphylococcus aureus induces a humoral immune response in serum and milk.
BMC Vet Res. 15 (1): 15. -
Pérez-caballero, R. et al. (2018) Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
Parasit Vectors. 11 (1): 640. -
Bloomer, S.A. et al. (2019) Aging results in accumulation of M1 and M2 hepatic macrophages and a differential response to gadolinium chloride.
Histochem Cell Biol. Nov 06 [Epub ahead of print]. -
Foulon, E. & Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
J Immunol Methods. 339 (1): 1-10. -
Brodzki, P. et al. (2019) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
Anim Sci J. Nov 06 [Epub ahead of print]. -
Gondaira, S. et al. (2020) Immunosuppression in Cows following Intramammary Infusion of Mycoplasma bovis.
Infect Immun. 88 (3)Feb 20 [Epub ahead of print]. -
Nakajima, N. et al. (2019) Effects of direct exposure to cold weather under grazing in winter on the physiological, immunological, and behavioral conditions of Japanese Black beef cattle in central Japan.
Anim Sci J. 90 (8): 1033-41. -
Risalde, M.A. et al. (2020) BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV.
Comp Immunol Microbiol Infect Dis. 68: 101388. -
Sirak, A. et al. (2021) Cellular and Cytokine Responses in Lymph Node Granulomas of Bacillus Calmette Guérin (BCG)-Vaccinated and Non-vaccinated Cross-Breed Calves Naturally Infected With Mycobacterium bovis.
Front Vet Sci. 8: 698800. -
Park, D.S. et al. (2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
Cell Stress Chaperones. 26 (4): 705-20. -
de Araújo, F.F. et al. (2019) Distinct immune response profile during Rhipicephalus (Boophilus) microplus. infestations of guzerat dairy herd according to the maternal lineage ancestry (mitochondrial DNA).
Vet Parasitol. 273: 36-44. -
Colombatti Olivieri, M.A. et al. (2021) Evaluation of a virulent strain of Mycobacterium avium. subsp. paratuberculosis. used as a heat-killed vaccine.
Vaccine. Nov 10;S0264-410X(21)01433-X. -
Kato-Mori, Y. et al. (2021) Characterization of a variant CD4 molecule in Japanese Black cattle.
Vet Immunol Immunopathol. 232: 110167. -
Bidart, J. et al. (2020) A New Cage-Like Particle Adjuvant Enhances Protection of Foot-and-Mouth Disease Vaccine.
Front Vet Sci. 7: 396. -
Damani-Yokota, P. et al. (2021) Transcriptional programming and gene regulation in WC1+ γδ T cell subpopulations.
Mol Immunol. 142: 50-62. -
Casaro, S. et al. (2022) Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytes
VetImmunol Immunopathol. 248: 110417. -
Kolar, Q.K. et al. (2020) Anatomical distribution of respiratory tract leukocyte cell subsets in neonatal calves.
Vet Immunol Immunopathol. 227: 110090. -
Elsayed, M.S.A.E. et al. (2022) Real-time PCR using atpE, conventional PCR targeting different regions of difference, and flow cytometry for confirmation of Mycobacterium bovis. in buffaloes and cattle from the Delta area of Egypt.
BMC Microbiol. 22 (1): 154.
- UniProt
- P31783
- Entrez Gene
- CD8A
- GO Terms
- GO:0016021 integral to membrane
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