CD63 antibody | CC25

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Mouse anti Bovine CD63

Product Type
Monoclonal Antibody
Clone
CC25
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2042GA F 0.1 mg
Mouse anti Bovine CD63 antibody, clone CC25 recognizes the bovine homologue of human CD63, a 237 amino acid multipass transmembrane glycoprotein and member of the tetraspanin TM4SF protein family with a predicted molecular weight of ~26 kDa. CD63 is also known as lysosome associated membrane glycoprotein 3 or LAMP-3.

CD63 along with other TM4SF members including CD9, CD61 and CD151 can form specific interactions with phosphoinositide 4-kinase, suggesting a role for CD63 in the recruitment of phosphoinositide 4-kinase to specific membrane sites (Yauch and Hemler 2000).

CD63 is expressed on the cell surface of platelets and basophils, along with activated macrophages, monocytes and granulocytes.

Mouse anti Bovine CD63, clone CC25 acts as a specific marker for bovine lysozomes and has been used for the identification and quantitation of phagosome-lysozome fusion in models of bacterial insult (Souza et al. 2013).

Product Details

Target Species
Bovine
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9XSK2 Related reagents
Entrez Gene
CD63 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005765 lysosomal membrane
GO:0031902 late endosome membrane
Regulatory
For research purposes only

Applications of CD63 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/25
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Immunofluorescence

Product Specific References

References for CD63 antibody

  1. Brooke, G.P. et al. (1999) Molecular cloning of cattle CD63 and evidence for high level expression on subpopulations of dendritic cells.
    Immunogenetics. 49 (9): 812-4.
  2. Colino, J. and Snapper, C.M. (2006) Exosomes from bone marrow dendritic cells pulsed with diphtheria toxoid preferentially induce type 1 antigen-specific IgG responses in naive recipients in the absence of free antigen.
    J Immunol. 177: 3757-62.
  3. Souza, C.D. et al. (2007) Role of the mitogen-activated protein kinase pathway in the differential response of bovine monocytes to Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
    Microbes Infect. 9: 1545-52.
  4. Souza, C. et al. (2013) Mannosylated lipoarabinomannans from Mycobacterium avium subsp. Paratuberculosis alters the inflammatory response by bovine macrophages and suppresses killing of mycobacterium avium subsp. Avium organisms.
    PLoS One 8: e75924.
  5. Wolf, T. et al. (2015) The Intestinal Transport of Bovine Milk Exosomes Is Mediated by Endocytosis in Human Colon Carcinoma Caco-2 Cells and Rat Small Intestinal IEC-6 Cells.
    J Nutr. 145 (10): 2201-6.
  6. Weiss, D.J. et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
    J Leukoc Biol. 83: 48-55.
  7. Kusuma, R.J. et al. (2016) Human vascular endothelial cells transport foreign exosomes from cow's milk by endocytosis.
    Am J Physiol Cell Physiol. Mar 16: ajpcell.00169.2015. [Epub ahead of print]
  8. Mobley, C.B. et al. (2016) Whey protein-derived exosomes increase protein synthesis and hypertrophy in C2C12 myotubes.
    Journal of Dairy Science. Oct 27 [Epub ahead of print]
  9. Carretta MD et al. (2016) Butyric acid stimulates bovine neutrophil functions and potentiates the effect of platelet activating factor.
    Vet Immunol Immunopathol. 176: 18-27.