CD63 antibody | CC25
Mouse anti Bovine CD63 antibody, clone CC25 (MCA2042GA) used to assess CD63 abundance in bovine BL20 cell extracts by western blotting.
Image caption:
EV shed by BL20 and TBL20 cells. Western blot analysis of 1. BL20 whole cell extract; 2. TBL20 whole cell extract; 3. BL‐EV (ultracentrifugation); 4. TBL‐EV (ultracentrifugation); 5. BL20 concentrated EV depleted supernatant; 6. TBL20 concentrated EV depleted supernatant. Antibodies for mouse anti bovine CD63 (1:500, AbD Serotec).
From:Gillan V, Simpson DM, Kinnaird J, Maitland K, Shiels B, Devaney E.
Characterisation of infection associated microRNA and protein cargo in extracellular vesicles of Theileria annulata infected leukocytes.
Cell Microbiol. 2019 Jan;21(1):e12969.
doi: 10.1111/cmi.12969.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine CD63
- Product Type
- Monoclonal Antibody
- Clone
- CC25
- Isotype
- IgG1
- Specificity
- CD63
Antibody Quality and Performance Guarantee
| Mouse anti Bovine CD63 antibody, clone CC25 recognizes the bovine homologue of human CD63, a 237 amino acid multipass transmembrane glycoprotein and member of the tetraspanin TM4SF protein family with a predicted molecular weight of ~26 kDa. CD63 is also known as lysosome associated membrane glycoprotein 3 or LAMP-3. CD63 along with other TM4SF members including CD9, CD61 and CD151 can form specific interactions with phosphoinositide 4-kinase, suggesting a role for CD63 in the recruitment of phosphoinositide 4-kinase to specific membrane sites (Yauch and Hemler 2000). CD63 is expressed on the cell surface of platelets and basophils, along with activated macrophages, monocytes and granulocytes. Mouse anti Bovine CD63, clone CC25 acts as a specific marker for bovine lysozomes and has been used for the identification and quantitation of phagosome-lysozome fusion in models of bacterial insult (Souza et al. 2013). |
- Target Species
- Bovine
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Bovine PBMC
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/25 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD63 antibody
-
Brooke, G.P. et al. (1999) Molecular cloning of cattle CD63 and evidence for high level expression on subpopulations of dendritic cells.
Immunogenetics. 49 (9): 812-4. -
Colino, J. and Snapper, C.M. (2006) Exosomes from bone marrow dendritic cells pulsed with diphtheria toxoid preferentially induce type 1 antigen-specific IgG responses in naive recipients in the absence of free antigen.
J Immunol. 177: 3757-62. -
Souza, C.D. et al. (2007) Role of the mitogen-activated protein kinase pathway in the differential response of bovine monocytes to Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
Microbes Infect. 9: 1545-52. -
Weiss, D.J. et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
J Leukoc Biol. 83: 48-55. -
Souza, C. et al. (2013) Mannosylated lipoarabinomannans from Mycobacterium avium subsp. Paratuberculosis alters the inflammatory response by bovine macrophages and suppresses killing of mycobacterium avium subsp. Avium organisms.
PLoS One 8: e75924. -
Wolf, T. et al. (2015) The Intestinal Transport of Bovine Milk Exosomes Is Mediated by Endocytosis in Human Colon Carcinoma Caco-2 Cells and Rat Small Intestinal IEC-6 Cells.
J Nutr. 145 (10): 2201-6. -
Carretta MD et al. (2016) Butyric acid stimulates bovine neutrophil functions and potentiates the effect of platelet activating factor.
Vet Immunol Immunopathol. 176: 18-27. -
Kusuma, R.J. et al. (2016) Human vascular endothelial cells transport foreign exosomes from cow's milk by endocytosis.
Am J Physiol Cell Physiol. 310 (10): C800-7.
View The Latest Product References
-
Mobley, C.B. et al. (2017) Whey protein-derived exosomes increase protein synthesis and hypertrophy in C2-C12 myotubes.
J Dairy Sci. 100 (1): 48-64. -
Gillan, V. et al. (2019) Characterisation of infection associated microRNA and protein cargo in extracellular vesicles of Theileria annulata. infected leukocytes.
Cell Microbiol. 21 (1): e12969. -
Fiorenza, M.F. et al. (2021) Neutrophils recognize and amplify IFNT signals derived from day 7 bovine embryo for stimulation of ISGs expression in vitro.: A possible implication for the early maternal recognition of pregnancy.
Biochem Biophys Res Commun. 553: 37-43.
- UniProt
- Q9XSK2
- Entrez Gene
- CD63
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005765 lysosomal membrane
- GO:0031902 late endosome membrane
MCA2042GA
154532
163140
1710
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