CD63 antibody | CC25
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|Mouse anti Bovine CD63 antibody, clone CC25 recognizes the bovine homologue of human CD63, a 237 amino acid multipass transmembrane glycoprotein and member of the tetraspanin TM4SF protein family with a predicted molecular weight of ~26 kDa. CD63 is also known as lysosome associated membrane glycoprotein 3 or LAMP-3.
CD63 along with other TM4SF members including CD9, CD61 and CD151 can form specific interactions with phosphoinositide 4-kinase, suggesting a role for CD63 in the recruitment of phosphoinositide 4-kinase to specific membrane sites (Yauch and Hemler 2000). CD63 is expressed on the cell surface of platelets and basophils, along with activated macrophages, monocytes and granulocytes.
Mouse anti Bovine CD63, clone CC25 acts as a specific marker for bovine lysozomes and has been used for the identification and quantitation of phagosome-lysozome fusion in models of bacterial insult (Souza et al. 2013).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Bovine PBMC
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD63 antibody
Brooke, G.P. et al. (1999) Molecular cloning of cattle CD63 and evidence for high level expression on subpopulations of dendritic cells.
Immunogenetics. 49 (9): 812-4.
Colino, J. and Snapper, C.M. (2006) Exosomes from bone marrow dendritic cells pulsed with diphtheria toxoid preferentially induce type 1 antigen-specific IgG responses in naive recipients in the absence of free antigen.
J Immunol. 177: 3757-62.
Souza, C.D. et al. (2007) Role of the mitogen-activated protein kinase pathway in the differential response of bovine monocytes to Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
Microbes Infect. 9: 1545-52.
Souza, C. et al. (2013) Mannosylated lipoarabinomannans from Mycobacterium avium subsp. Paratuberculosis alters the inflammatory response by bovine macrophages and suppresses killing of mycobacterium avium subsp. Avium organisms.
PLoS One 8: e75924.
Wolf, T. et al. (2015) The Intestinal Transport of Bovine Milk Exosomes Is Mediated by Endocytosis in Human Colon Carcinoma Caco-2 Cells and Rat Small Intestinal IEC-6 Cells.
J Nutr. 145 (10): 2201-6.
Weiss, D.J. et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
J Leukoc Biol. 83: 48-55.
Kusuma, R.J. et al. (2016) Human vascular endothelial cells transport foreign exosomes from cow's milk by endocytosis.
Am J Physiol Cell Physiol. 310 (10): C800-7.
Carretta MD et al. (2016) Butyric acid stimulates bovine neutrophil functions and potentiates the effect of platelet activating factor.
Vet Immunol Immunopathol. 176: 18-27.
View The Latest Product References
Mobley, C.B. et al. (2017) Whey protein-derived exosomes increase protein synthesis and hypertrophy in C2-C12 myotubes.
J Dairy Sci. 100 (1): 48-64.
Gillan, V. et al. (2019) Characterisation of infection associated microRNA and protein cargo in extracellular vesicles of Theileria annulata. infected leukocytes.
Cell Microbiol. 21 (1): e12969.
Fiorenza, M.F. et al. (2021) Neutrophils recognize and amplify IFNT signals derived from day 7 bovine embryo for stimulation of ISGs expression in vitro.: A possible implication for the early maternal recognition of pregnancy.
Biochem Biophys Res Commun. 553: 37-43.
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