CD45 antibody | CC1
Mouse anti Bovine CD45 antibody, clone CC1 recognizes the bovine homologue of the human CD45 antigen, also known as leucocyte common antigen (LCA), a pan leucocyte cell surface marker expressed on all cells of hematopoietic origin except for erythrocytes (Bembridge et al. 1993).
CD45 occurs in a number of isoforms, which in bovine includes CD45R, CD45RA, CD45RB and CD45RO and which have restricted cellular expression.
CD45 occurs in a number of isoforms, which in bovine includes CD45R, CD45RA, CD45RB and CD45RO and which have restricted cellular expression.
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Bovine thoracic duct lymphocytes
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD45 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/25 | 1/200 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Bovine CD45RB | MCA1650GA | F | 0.1 mg |
|
|
| Mouse anti Bovine CD45RO | MCA2434GA | F IP | 0.1 mg |
|
Product Specific References
References for CD45 antibody
-
Bembridge, G.P. et al. (1993) Identification of monoclonal antibodies specific for bovine leukocyte common antigen (CD45) together with a novel broadly expressed leukocyte differentiation antigen, BoWC11.
Vet Immunol Immunopathol. 39: 115-20. -
Inchaisri, C. et al. (2000) Studies on the modulation of leucocyte subpopulations and immunoglobulins following intramammary infusion of beta 1,3-glucan into the bovine udder during the dry period.
J Vet Med B Infect Dis Vet Public Health. 47 (5): 373-86. -
Hu, S. et al. (2001) Effect of subcutaneous injection of ginseng on cows with subclinical Staphylococcus aureus mastitis.
J Vet Med B Infect Dis Vet Public Health. 48 (7): 519-28. -
Gånheim, C. et al. (2005) Changes in peripheral blood leucocyte counts and subpopulations after experimental infection with BVDV and/or Mannheimia haemolytica.
J Vet Med B Infect Dis Vet Public Health. 52 (9): 380-5. -
Niku, M. et al. (2006) Identification of major cell types in paraffin sections of bovine tissues.
BMC Vet Res. 2: 5. -
Grönlund, U. et al. (2006) Changes in blood and milk lymphocyte sub-populations during acute and chronic phases of Staphylococcus aureus induced bovine mastitis.
Res Vet Sci. 80 (2): 147-54. -
Galatowicz, G. et al. (2007) Ocular anti-allergic compounds selectively inhibit human mast cell cytokines in vitro and conjunctival cell infiltration in vivo.
Clin Exp Allergy. 37 (11): 1648-56. -
Riondato, F. et al. (2008) Effects of road transportation on lymphocyte subsets in calves.
Vet J. 175 (3): 364-8. -
Huang, L. et al. (2010) A hierarchy of endothelial colony-forming cell activity displayed by bovine corneal endothelial cells.
Invest Ophthalmol Vis Sci. 51: 3943-9. -
Spalenza, V. et al. (2011) Identification of internal control genes for quantitative expression analysis by real-time PCR in bovine peripheral lymphocytes.
Vet J. 189 (3): 278-83. -
Lynch, E.M. et al. (2012) Effect of pre-weaning concentrate supplementation on peripheral distribution of leukocytes, functional activity of neutrophils, acute phase protein and behavioural responses of abruptly weaned and housed beef calves.
BMC Veterinary Research 8: 1 -
Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis.
Sci Rep. 7 (1): 3441.
Further Reading
-
Ballingall, K.T. et al. (2001) The CD45 locus in cattle: allelic polymorphism and evidence for exceptional positive natural selection.
Immunogenetics. 52: 276-83.
