CD45 antibody | CC1

Product Code Applications Pack Size List Price Your Price Qty
MCA832GA
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SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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loader
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Mouse anti Bovine CD45 antibody, clone CC1 recognizes the bovine homologue of the human CD45 antigen, also known as leucocyte common antigen (LCA),

CD45 is a pan leucocyte cell surface marker expressed on all cells of hematopoietic origin except for erythrocytes (Bembridge et al. 1993). CD45 occurs in a number of isoforms, which in bovine includes CD45R, CD45RA, CD45RB and CD45RO and which have restricted cellular expression.

Target Species
Bovine
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Bovine thoracic duct lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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References for CD45 antibody

  1. Bembridge, G.P. et al. (1993) Identification of monoclonal antibodies specific for bovine leukocyte common antigen (CD45) together with a novel broadly expressed leukocyte differentiation antigen, BoWC11.
    Vet Immunol Immunopathol. 39: 115-20.
  2. Inchaisri, C. et al. (2000) Studies on the modulation of leucocyte subpopulations and immunoglobulins following intramammary infusion of beta 1,3-glucan into the bovine udder during the dry period.
    J Vet Med B Infect Dis Vet Public Health. 47 (5): 373-86.
  3. Hu, S. et al. (2001) Effect of subcutaneous injection of ginseng on cows with subclinical Staphylococcus aureus mastitis.
    J Vet Med B Infect Dis Vet Public Health. 48 (7): 519-28.
  4. Gånheim, C. et al. (2005) Changes in peripheral blood leucocyte counts and subpopulations after experimental infection with BVDV and/or Mannheimia haemolytica.
    J Vet Med B Infect Dis Vet Public Health. 52 (9): 380-5.
  5. Niku, M. et al. (2006) Identification of major cell types in paraffin sections of bovine tissues.
    BMC Vet Res. 2: 5.
  6. Grönlund, U. et al. (2006) Changes in blood and milk lymphocyte sub-populations during acute and chronic phases of Staphylococcus aureus induced bovine mastitis.
    Res Vet Sci. 80 (2): 147-54.
  7. Galatowicz, G. et al. (2007) Ocular anti-allergic compounds selectively inhibit human mast cell cytokines in vitro and conjunctival cell infiltration in vivo.
    Clin Exp Allergy. 37 (11): 1648-56.
  8. Riondato, F. et al. (2008) Effects of road transportation on lymphocyte subsets in calves.
    Vet J. 175 (3): 364-8.
  9. View The Latest Product References
  10. Huang, L. et al. (2010) A hierarchy of endothelial colony-forming cell activity displayed by bovine corneal endothelial cells.
    Invest Ophthalmol Vis Sci. 51: 3943-9.
  11. Spalenza, V. et al. (2011) Identification of internal control genes for quantitative expression analysis by real-time PCR in bovine peripheral lymphocytes.
    Vet J. 189 (3): 278-83.
  12. Lynch, E.M. et al. (2012) Effect of pre-weaning concentrate supplementation on peripheral distribution of leukocytes, functional activity of neutrophils, acute phase protein and behavioural responses of abruptly weaned and housed beef calves.
    BMC Veterinary Research 8: 1
  13. Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis.
    Sci Rep. 7 (1): 3441.
  14. Lee, J. et al. (2020) Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells.
    Biosci Rep. 40 (4): BSR20181829.

Further Reading

  1. Ballingall, K.T. et al. (2001) The CD45 locus in cattle: allelic polymorphism and evidence for exceptional positive natural selection.
    Immunogenetics. 52: 276-83.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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