CD45 antibody | CC1
FITC conjugated Mouse anti Bovine CD45 antibody, clone CC1 (MCA832F) used to assess CD44 on bovine mesenchymal stem cells by flow cytometry. Shown with a positive marker of bovine MSCs, CD44 using phycoerythrin conjugated Mouse anti Bovine CD44 antibody, clone IL-A118.
Image caption:
Cell surface markers of boT-MSCs.
Cell surface markers (positive: CD44, CD90 negative: CD45) observed (P3) by flow cytometry analysis. Data are expressed as means ± standard error of values, and were obtained by three determinations.
From: Lee J, Byeon JS, Gu NY, Lee S, Lee SA, Jeong DU, Ouh IO, Cho IS, Song JY, Lee YH, Hyun BH.
Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells.
Biosci Rep. 2020 Apr 30;40(4):BSR20181829.
doi: 10.1042/BSR20181829.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine CD45 antibody, clone CC1 recognizes the bovine homologue of the human CD45 antigen, also known as leucocyte common antigen (LCA), a pan leucocyte cell surface marker expressed on all cells of hematopoietic origin except for erythrocytes (Bembridge et al. 1993).
CD45 occurs in a number of isoforms, which in bovine includes CD45R, CD45RA, CD45RB and CD45RO and which have restricted cellular expression.
CD45 occurs in a number of isoforms, which in bovine includes CD45R, CD45RA, CD45RB and CD45RO and which have restricted cellular expression.
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Bovine thoracic duct lymphocytes
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD45 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/25 | 1/200 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Bovine CD45RB | MCA1650GA | F | 0.1 mg |
|
|
| Mouse anti Bovine CD45RO | MCA2434GA | F IP | 0.1 mg |
|
Product Specific References
References for CD45 antibody
-
Bembridge, G.P. et al. (1993) Identification of monoclonal antibodies specific for bovine leukocyte common antigen (CD45) together with a novel broadly expressed leukocyte differentiation antigen, BoWC11.
Vet Immunol Immunopathol. 39: 115-20. -
Inchaisri, C. et al. (2000) Studies on the modulation of leucocyte subpopulations and immunoglobulins following intramammary infusion of beta 1,3-glucan into the bovine udder during the dry period.
J Vet Med B Infect Dis Vet Public Health. 47 (5): 373-86. -
Hu, S. et al. (2001) Effect of subcutaneous injection of ginseng on cows with subclinical Staphylococcus aureus mastitis.
J Vet Med B Infect Dis Vet Public Health. 48 (7): 519-28. -
Gånheim, C. et al. (2005) Changes in peripheral blood leucocyte counts and subpopulations after experimental infection with BVDV and/or Mannheimia haemolytica.
J Vet Med B Infect Dis Vet Public Health. 52 (9): 380-5. -
Niku, M. et al. (2006) Identification of major cell types in paraffin sections of bovine tissues.
BMC Vet Res. 2: 5. -
Grönlund, U. et al. (2006) Changes in blood and milk lymphocyte sub-populations during acute and chronic phases of Staphylococcus aureus induced bovine mastitis.
Res Vet Sci. 80 (2): 147-54. -
Galatowicz, G. et al. (2007) Ocular anti-allergic compounds selectively inhibit human mast cell cytokines in vitro and conjunctival cell infiltration in vivo.
Clin Exp Allergy. 37 (11): 1648-56. -
Riondato, F. et al. (2008) Effects of road transportation on lymphocyte subsets in calves.
Vet J. 175 (3): 364-8. -
Huang, L. et al. (2010) A hierarchy of endothelial colony-forming cell activity displayed by bovine corneal endothelial cells.
Invest Ophthalmol Vis Sci. 51: 3943-9. -
Spalenza, V. et al. (2011) Identification of internal control genes for quantitative expression analysis by real-time PCR in bovine peripheral lymphocytes.
Vet J. 189 (3): 278-83. -
Lynch, E.M. et al. (2012) Effect of pre-weaning concentrate supplementation on peripheral distribution of leukocytes, functional activity of neutrophils, acute phase protein and behavioural responses of abruptly weaned and housed beef calves.
BMC Veterinary Research 8: 1 -
Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis.
Sci Rep. 7 (1): 3441. -
Lee, J. et al. (2020) Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells.
Biosci Rep. 40 (4): BSR20181829.
Further Reading
-
Ballingall, K.T. et al. (2001) The CD45 locus in cattle: allelic polymorphism and evidence for exceptional positive natural selection.
Immunogenetics. 52: 276-83.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
