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CD32 antibody | CCG36

Mouse anti Bovine CD32:FITC

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price

Mouse anti Bovine CD32 antibody, clone CCG36 recognizes the bovine homologue of human CD32, one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. Bovine CD32 is a single pass type 1 membrane protein of approximately 32kDa, expressed on the cell surface of most cells including B-lymphocytes, monocytes, neutrophils and afferent veiled lymph dendritic cells Chattha, K. et al. 2010. It has been shown that expression of bovine CD32 is higher on macrophages than on neutrophils.

CD32 can function in an inhibitory capacity to antibody production and is the low affinity Fc receptor for IgG (FcRII), binding to the Fc region of immunoglobulin gamma Chattha et al. 2009.

Mouse anti Bovine CD32, clone CCG36 is one of a number of anti bovine CD32 reagents available from Bio-Rad, clone CCG36 is of interest in that it also recognizes ovine CD32 while clone CCG39 recognizes only bovine CD32.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Bovine FcγRII-transfected COS7 cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS-1myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
List Price Your Price
Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Bovine CD32:FITC MCA5919F F 0.1 mg loader
List Price Your Price
Description Mouse anti Bovine CD32:FITC
Mouse anti Bovine CD32 MCA5919GA C F 0.1 mg loader
List Price Your Price
Description Mouse anti Bovine CD32

References for CD32 antibody

  1. Chattha, K.S. et al. (2009) Age related variation in expression of CD21 and CD32 on bovine lymphocytes: a cross-sectional study.
    Vet Immunol Immunopathol. 130 (1-2): 70-8.
  2. Chattha, K.S. et al. (2010) Variation in expression of membrane IgM, CD21 (CR2) and CD32 (Fcgamma RIIB) on bovine lymphocytes with age: a longitudinal study.
    Dev Comp Immunol. 34 (5): 510-7.
  3. Chattha, K.S. et al. (2010) Expression of complement receptor 2 (CD21), membrane IgM and the inhibitory receptor CD32 (FcgammaRIIb) in the lymphoid tissues of neonatal calves.
    Vet Immunol Immunopathol. 137 (1-2): 99-108.
  4. Chattha, K.S. et al. (2010) Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves.
    Vet Immunol Immunopathol. 137 (3-4): 284-90.
  5. Werling, D. et al. (1998) Analysis of the phenotype and phagocytic activity of monocytes/macrophages from cattle infected with the bovine leukaemia virus.
    Vet Immunol Immunopathol. 62 (3): 185-95.

Entrez Gene
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0019864 IgG binding



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