CD32 antibody | CCG36
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Mouse anti Bovine CD32:FITC
- Product Type
- Monoclonal Antibody
|Mouse anti Bovine CD32 antibody, clone CCG36 recognizes the bovine homologue of human CD32, one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. Bovine CD32 is a single pass type 1 membrane protein of approximately 32kDa, expressed on the cell surface of most cells including B-lymphocytes, monocytes, neutrophils and afferent veiled lymph dendritic cells Chattha, K. et al. 2010. It has been shown that expression of bovine CD32 is higher on macrophages than on neutrophils.
CD32 can function in an inhibitory capacity to antibody production and is the low affinity Fc receptor for IgG (FcRII), binding to the Fc region of immunoglobulin gamma Chattha et al. 2009.
Mouse anti Bovine CD32, clone CCG36 is one of a number of anti bovine CD32 reagents available from Bio-Rad, clone CCG36 is of interest in that it also recognizes ovine CD32 while clone CCG39 recognizes only bovine CD32.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Sheep
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- Bovine FcγRII-transfected COS7 cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS-1myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
|Description||Product Code||Applications||Pack Size||List Price||Your Price||Quantity|
|Mouse anti Bovine CD32:FITC||MCA5919F||F||0.1 mg||Log in|
|List Price||Your Price|
|Description||Mouse anti Bovine CD32:FITC|
|Mouse anti Bovine CD32||MCA5919GA||C F||0.1 mg||Log in|
|List Price||Your Price|
|Description||Mouse anti Bovine CD32|
References for CD32 antibody
Chattha, K.S. et al. (2009) Age related variation in expression of CD21 and CD32 on bovine lymphocytes: a cross-sectional study.
Vet Immunol Immunopathol. 130 (1-2): 70-8.
Chattha, K.S. et al. (2010) Variation in expression of membrane IgM, CD21 (CR2) and CD32 (Fcgamma RIIB) on bovine lymphocytes with age: a longitudinal study.
Dev Comp Immunol. 34 (5): 510-7.
Chattha, K.S. et al. (2010) Expression of complement receptor 2 (CD21), membrane IgM and the inhibitory receptor CD32 (FcgammaRIIb) in the lymphoid tissues of neonatal calves.
Vet Immunol Immunopathol. 137 (1-2): 99-108.
Chattha, K.S. et al. (2010) Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves.
Vet Immunol Immunopathol. 137 (3-4): 284-90.
Werling, D. et al. (1998) Analysis of the phenotype and phagocytic activity of monocytes/macrophages from cattle infected with the bovine leukaemia virus.
Vet Immunol Immunopathol. 62 (3): 185-95.
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