CD172a antibody | DH59B

100% Secure


Mouse anti Bovine CD172a

Product Type
Monoclonal Antibody
Clone
DH59B
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6079 C F 0.1 mg
Mouse anti Bovine CD172a, clone DH59B, recognizes bovine CD172a also known as SIRP alpha or MyD-1 antigen. CD172a is a transmembrane signal regulatory protein expressed primarily by macrophages, monocytes, dendritic cells, granulocytes, myeloid progenitors, hematopoietic stem cells, and neurons (Barclay et al. 2006). The extracellular region of SIRP family consists of three immunoglobulin superfamily (IgSF) domains; two IgC and one IgV domain (Barclay et al. 2006).

The IgV domain of CD172a binds to CD47 (Hatherley et al. 2007).The binding domain of CD172a is analogous to that of immunoglobulins and T cell receptors and is involved in myeloid cell activation (Berg et al.2004). However, signaling via CD172a is mainly inhibitory to cell function and phagocytosis (Oldenborg et al. 2001). CD172a expressing cells are stimulated in Mycobacterium tuberculosis infection, influencing migration of dendritic cells and macrophages, phagocytosis, and granuloma formation (Waters et al. 2009).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bison
Water Buffalo
Sheep
Horse
Dog
Cat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Concentrated tissue culture supernatant - liquid
Preparation
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
Buffer Solution
Serum free tissue culture medium containing proprietary protein free supplement
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Cells from multiple species with the final screening of the fusion on cells from dog
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
O46631 Related reagents
Entrez Gene
SIRPA Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0017124 SH3 domain binding
Regulatory
For research purposes only

Applications of CD172a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Bovine CD14 MCA6085 0.1 mg F

Product Specific References

References for CD172a antibody

  1. Herrmann L.M. et al. (2003) CD21-positive follicular dendritic cells: A possible source of PrPSc in lymph node macrophages of scrapie-infected sheep.
    Am J Pathol. 162 (4): 1075-81
  2. Davis W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
    Vet Immunol Immunopathol. 119 (1-2): 123-30.
  3. Ibrahim S. et al. (2007) Screening of anti-human leukocyte monoclonal antibodies for reactivity with equine leukocytes.
    Vet Immunol Immunopathol. 119 (1-2): 63-80.
  4. Mérant C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
    Vet Immunol Immunopathol. 131 (1-2): 1-8.
  5. Contreras G.A. et al. (2010) Lipomobilization in periparturient dairy cows influences the composition of plasma nonesterified fatty acids and leukocyte phospholipid fatty acids.
    J Dairy Sci. 93 (6): 2508-16.
  6. Herrmann-Hoesing L.M. et al. (2010) Ovine progressive pneumonia virus capsid antigen as found in CD163- and CD172a-positive alveolar macrophages of persistently infected sheep.
    Vet Pathol. 47 (3): 518-28.