Mouse anti Bovine CD172a, clone DH59B
, recognizes bovine CD172a also known as SIRP alpha or MyD-1 antigen. CD172a is a transmembrane signal regulatory protein expressed primarily by macrophages, monocytes, dendritic cells, granulocytes, myeloid progenitors, hematopoietic stem cells, and neurons (Barclay et al. 2006
). The extracellular region of SIRP family consists of three immunoglobulin superfamily (IgSF) domains; two IgC and one IgV domain (Barclay et al. 2006
The IgV domain of CD172a binds to CD47 (Hatherley et al. 2007
).The binding domain of CD172a is analogous to that of immunoglobulins and T cell receptors and is involved in myeloid cell activation (Berg et al.2004
). However, signaling via CD172a is mainly inhibitory to cell function and phagocytosis (Oldenborg et al. 2001
). CD172a expressing cells are stimulated in Mycobacterium tuberculosis
infection, influencing migration of dendritic cells and macrophages, phagocytosis, and granuloma formation (Waters et al. 2009
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD172a antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul