CD172a antibody | DH59B
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|Mouse anti Bovine CD172a, clone DH59B, recognizes bovine CD172a also known as SIRP alpha or MyD-1 antigen. CD172a is a transmembrane signal regulatory protein expressed primarily by macrophages, monocytes, dendritic cells, granulocytes, myeloid progenitors, hematopoietic stem cells, and neurons (Barclay et al. 2006). The extracellular region of SIRP family consists of three immunoglobulin superfamily (IgSF) domains; two IgC and one IgV domain (Barclay et al. 2006).
The IgV domain of CD172a binds to CD47 (Hatherley et al. 2007).The binding domain of CD172a is analogous to that of immunoglobulins and T cell receptors and is involved in myeloid cell activation (Berg et al.2004). However, signaling via CD172a is mainly inhibitory to cell function and phagocytosis (Oldenborg et al. 2001). CD172a expressing cells are stimulated in Mycobacterium tuberculosis infection, influencing migration of dendritic cells and macrophages, phagocytosis, and granuloma formation (Waters et al. 2009).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Bison Water Buffalo Sheep Horse Dog Cat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Concentrated tissue culture supernatant - liquid
- Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
- Buffer Solution
- Serum free tissue culture medium containing proprietary protein free supplement
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Cells from multiple species with the final screening of the fusion on cells from dog
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
References for CD172a antibody
Herrmann L.M. et al. (2003) CD21-positive follicular dendritic cells: A possible source of PrPSc in lymph node macrophages of scrapie-infected sheep.
Am J Pathol. 162 (4): 1075-81
Davis W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
Vet Immunol Immunopathol. 119 (1-2): 123-30.
Ibrahim S. et al. (2007) Screening of anti-human leukocyte monoclonal antibodies for reactivity with equine leukocytes.
Vet Immunol Immunopathol. 119 (1-2): 63-80.
Mérant C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
Vet Immunol Immunopathol. 131 (1-2): 1-8.
Contreras G.A. et al. (2010) Lipomobilization in periparturient dairy cows influences the composition of plasma nonesterified fatty acids and leukocyte phospholipid fatty acids.
J Dairy Sci. 93 (6): 2508-16.
Herrmann-Hoesing L.M. et al. (2010) Ovine progressive pneumonia virus capsid antigen as found in CD163- and CD172a-positive alveolar macrophages of persistently infected sheep.
Vet Pathol. 47 (3): 518-28.
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