Mouse anti bovine CD13, clone CC81, recognises bovine CD13, a 150 kDa type II membrane protein shown to be a metallopeptidase in humans. In cattle the antigen recognised by clone CC81 is primarily expressed on enterocytes and cells with a dendritic morphology in the small intestine. Clone CC81 also defines a subpopulation of dendritic cells in afferent lymph that are CC81 Ag +ve and SIRPalpha -ve, which show differences in their capacities to stimulate T cells and cytokine synthesis compared to the CC81 Ag –ve SIRPalpha +ve dendritic cells.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Cells from cattle intestine.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
integral to membrane
zinc ion binding
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD13 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for CD13 antibody
Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.
J Immunol. 159 (11): 5372-82.
Hope, J.C. et al. (2001) Differences in the induction of CD8+ T cell responses by subpopulations of dendritic cells from afferent lymph are related to IL-1 alpha secretion.
J Leukoc Biol. 69 (2): 271-9.
Stephens, S. A. et al. (2003) Differences in cytokine synthesis by the sub-populations of dendritic cells from afferent lymph.
Immunology. 110: 48-57.
Bastos, R.G. et al. (2008) Bovine NK cells acquire cytotoxic activity and produce IFN-gamma after stimulation by Mycobacterium bovis BCG or Babesia bovis-exposed splenic dendritic cells.
Vet Immunol Immunopathol. 124: 302-12.
Schneider DA et al. (2011) Dynamics of bovine spleen cell populations during the acute response to Babesia bovis infection: an immunohistological study.
Parasite Immunol. 33 (1): 34-44.
Fries, P.N. et al. (2011) Age-related changes in the distribution and frequency of myeloid and T cell populations in the small intestine of calves.
Cell Immunol. 271 (2): 428-37.
Fries, P. et al. (2011) Mucosal dendritic cell subpopulations in the small intestine of newborn calves.
Dev Comp Immunol. 35 (10): 1040-51.
Toka, F.N. et al. (2011) Rapid and transient activation of γδ T cells to IFN-γ production, NK cell-like killing, and antigen processing during acute virus infection.
J Immunol. 186 (8): 4853-61.