CD13 antibody | CC81

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Mouse anti Bovine CD13

Product Type
Monoclonal Antibody
Clone
CC81
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2338GA F 0.1 mg
Mouse anti bovine CD13, clone CC81, recognises bovine CD13, a 150 kDa type II membrane protein shown to be a metallopeptidase in humans. In cattle the antigen recognised by clone CC81 is primarily expressed on enterocytes and cells with a dendritic morphology in the small intestine. Clone CC81 also defines a subpopulation of dendritic cells in afferent lymph that are CC81 Ag +ve and SIRPalpha -ve, which show differences in their capacities to stimulate T cells and cytokine synthesis compared to the CC81 Ag –ve SIRPalpha +ve dendritic cells.

Product Details

Target Species
Bovine
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Cells from cattle intestine.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P79098 Related reagents
Entrez Gene
ANPEP Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0004177 aminopeptidase activity
GO:0008237 metallopeptidase activity
GO:0016021 integral to membrane
GO:0006508 proteolysis
GO:0008270 zinc ion binding
GO:0030154 cell differentiation
Regulatory
For research purposes only

Applications of CD13 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD13 antibody

  1. Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.
    J Immunol. 159 (11): 5372-82.
  2. Hope, J.C. et al. (2001) Differences in the induction of CD8+ T cell responses by subpopulations of dendritic cells from afferent lymph are related to IL-1 alpha secretion.
    J Leukoc Biol. 69 (2): 271-9.
  3. Stephens, S. A. et al. (2003) Differences in cytokine synthesis by the sub-populations of dendritic cells from afferent lymph.
    Immunology. 110: 48-57.
  4. Bastos, R.G. et al. (2008) Bovine NK cells acquire cytotoxic activity and produce IFN-gamma after stimulation by Mycobacterium bovis BCG or Babesia bovis-exposed splenic dendritic cells.
    Vet Immunol Immunopathol. 124: 302-12.
  5. Schneider DA et al. (2011) Dynamics of bovine spleen cell populations during the acute response to Babesia bovis infection: an immunohistological study.
    Parasite Immunol. 33 (1): 34-44.
  6. Fries, P.N. et al. (2011) Age-related changes in the distribution and frequency of myeloid and T cell populations in the small intestine of calves.
    Cell Immunol. 271 (2): 428-37.
  7. Fries, P. et al. (2011) Mucosal dendritic cell subpopulations in the small intestine of newborn calves.
    Dev Comp Immunol. 35 (10): 1040-51.
  8. Toka, F.N. et al. (2011) Rapid and transient activation of γδ T cells to IFN-γ production, NK cell-like killing, and antigen processing during acute virus infection.
    J Immunol. 186 (8): 4853-61.