CD13 antibody | CC81
Mouse anti Bovine CD13
- Product Type
- Monoclonal Antibody
- Clone
- CC81
- Isotype
- IgG1
- Specificity
- CD13
Antibody Quality and Performance Guarantee
| Mouse anti bovine CD13, clone CC81, recognises bovine CD13, a 150 kDa type II membrane protein shown to be a metallopeptidase in humans. In cattle the antigen recognised by clone CC81 is primarily expressed on enterocytes and cells with a dendritic morphology in the small intestine. Clone CC81 also defines a subpopulation of dendritic cells in afferent lymph that are CC81 Ag +ve and SIRPalpha -ve, which show differences in their capacities to stimulate T cells and cytokine synthesis compared to the CC81 Ag –ve SIRPalpha +ve dendritic cells. |
- Target Species
- Bovine
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Cells from cattle intestine.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/1000 |
| Immunohistology - Frozen | |
1/25 | 1/50 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD13 antibody
-
Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.
J Immunol. 159 (11): 5372-82. -
Hope, J.C. et al. (2001) Differences in the induction of CD8+ T cell responses by subpopulations of dendritic cells from afferent lymph are related to IL-1 alpha secretion.
J Leukoc Biol. 69 (2): 271-9. -
Stephens, S. A. et al. (2003) Differences in cytokine synthesis by the sub-populations of dendritic cells from afferent lymph.
Immunology. 110: 48-57. -
Bastos, R.G. et al. (2008) Bovine NK cells acquire cytotoxic activity and produce IFN-gamma after stimulation by Mycobacterium bovis BCG or Babesia bovis-exposed splenic dendritic cells.
Vet Immunol Immunopathol. 124: 302-12. -
Schneider DA et al. (2011) Dynamics of bovine spleen cell populations during the acute response to Babesia bovis infection: an immunohistological study.
Parasite Immunol. 33 (1): 34-44. -
Fries, P.N. et al. (2011) Age-related changes in the distribution and frequency of myeloid and T cell populations in the small intestine of calves.
Cell Immunol. 271 (2): 428-37. -
Fries, P. et al. (2011) Mucosal dendritic cell subpopulations in the small intestine of newborn calves.
Dev Comp Immunol. 35 (10): 1040-51. -
Toka, F.N. et al. (2011) Rapid and transient activation of γδ T cells to IFN-γ production, NK cell-like killing, and antigen processing during acute virus infection.
J Immunol. 186 (8): 4853-61.
View The Latest Product References
- UniProt
- P79098
- Entrez Gene
- ANPEP
- GO Terms
- GO:0001525 angiogenesis
- GO:0004177 aminopeptidase activity
- GO:0008237 metallopeptidase activity
- GO:0016021 integral to membrane
- GO:0006508 proteolysis
- GO:0008270 zinc ion binding
- GO:0030154 cell differentiation
MCA2338GA
156910
1601
167942
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