IgG IgA IgM antibody | K185 4D4
Mouse anti Cat IgG/A/M, clone K185 4D4 recognizes feline immunoglobulins, binding to the IgA, IgG, and IgM classes.
In cat serum and mucosal secretions IgG, IgM, and IgA are the three major classes of immunoglobulins present. In serum IgG is the most abundant, followed by IgA and IgM (Harley et al.1998). However, in feline saliva IgA is found at a higher concentration than IgG, with IgM at the lowest levels (Harley et al.1998).
In cat serum and mucosal secretions IgG, IgM, and IgA are the three major classes of immunoglobulins present. In serum IgG is the most abundant, followed by IgA and IgM (Harley et al.1998). However, in feline saliva IgA is found at a higher concentration than IgG, with IgM at the lowest levels (Harley et al.1998).
Product Details
- Target Species
- Cat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Feline IgM
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgG IgA IgM antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2,000 | 1/20,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for IgG IgA IgM antibody
-
Norris, C.R. et al. (2003) Production of polyclonal antisera against feline immunoglobulin E and its use
in an ELISA in cats with experimentally induced asthma.
Vet Immunol Immunopathol. 96: 149-57.
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