IgG IgA IgM antibody | K185 4D4

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Mouse anti CAT IgG/A/M

Product Type
Monoclonal Antibody
Clone
K185 4D4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA623GA E 0.1 mg
Mouse anti Cat IgG/A/M, clone K185 4D4 recognizes feline immunoglobulins, binding to the IgA, IgG, and IgM classes.

In cat serum and mucosal secretions IgG, IgM, and IgA are the three major classes of immunoglobulins present. In serum IgG is the most abundant, followed by IgA and IgM (Harley et al.1998). However, in feline saliva IgA is found at a higher concentration than IgG, with IgM at the lowest levels (Harley et al.1998).

Product Details

Target Species
Cat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Feline IgM
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
18 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of IgG IgA IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2,000 1/20,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for IgG IgA IgM antibody

  1. Norris, C.R. et al. (2003) Production of polyclonal antisera against feline immunoglobulin E and its use in an ELISA in cats with experimentally induced asthma.
    Vet Immunol Immunopathol. 96: 149-57.