CD3 Antibody | CD3-12

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CD3 Antibody | CD3-12 gallery image 1

Formalin fixed, paraffin embedded human tonsil stained with Rat anti Human CD3 (MCA1477) following heat mediated antigen retrieval in EDTA buffer pH9.0

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CD3 Antibody | CD3-12 gallery image 2

Western blot analysis of Jurkat human acute T-cell leukemia whole cell lysate probed with Rat anti Human CD3 antibody (MCA1477) followed by HRP conjugated Goat anti Rat IgG, visualized by chemiluminescence

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CD3 Antibody | CD3-12 gallery image 3

ELISA analysis of human CD3 expression using Rat anti Human CD3ε, clone CD3-12 as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 (MCA643G) as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A serum (green) sample is included undiluted

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CD3 Antibody | CD3-12 gallery image 4

Published customer image:
Rat anti Human CD3 antibody, clone CD3-12 used for the detection of infiltrating T cells in formalin fixed, paraffin embedded kidney tissue sections.
Image caption:
Immunohistochemistry for inflammatory cells in kidneys of normal control mice, vehicle-treated mice with anti-GBM-GN, and EGCG-treated mice with anti-GBM-GN. Kidneys from mice 4 weeks after induction of anti-GBM-GN with or without EGCG treatment and kidneys from normal control mice were evaluated for inflammatory cell infiltration. Immunohistochemical analyses showed markedly increased interstitial infiltration of lymphocytes and macrophages in the vehicle-treated group (original magnification x 400). In contrast, the EGCG-treated animals exhibited significantly less interstitial infiltration of lymphocytes and macrophages. No significant inflammatory cell infiltrates were observed in the normal control mice. The number of lymphocyte and macrophages in glomeruli and interstitial compartment are depicted in the bar graphs. Values are mean ± SEM; n = 8–10 in each group.

From: Ye T, Zhen J, Du Y, Zhou JK, Peng A, et al. (2015) Green Tea Polyphenol (-)-Epigallocatechin-3-Gallate Restores Nrf2 Activity and Ameliorates Crescentic Glomerulonephritis.
PLoS ONE 10(3): e0119543.

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CD3 Antibody | CD3-12 gallery image 5

Published customer image:
Rat anti Human CD3 antibody, clone CD3-12 used for the detection of CD3 expressing cells in murine tissues by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Insert image caption

From: Flatz L, Rieger T, Merkler D, Bergthaler A, Regen T, et al. (2010) T Cell-Dependence of Lassa Fever Pathogenesis.
PLoS Pathog 6(3): e1000836.

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CD3 Antibody | CD3-12 gallery image 6

Published customer image:
Rat anti Mouse CD3 antibody, clone CD3-12 used dor the detection of CD3 expressing cells in human spleen by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Spectral imaging analysis of human spleen stained for CD3 (Warp Red), CD37 (True Blue) and cell nuclei (Nuclear Red). a Representative original multispectral image. Scale bar = 100 µm. Composite RGB image (b) of unmixed CD3 (c in red), CD37 (d in blue) and Nuclear Red (e in green in composite RGB image) signal using the spectral library (Fig. 4a). f Tissue segmentation; B cell follicle (B, yellow), T cell zone (T, red), red pulp (RP, green) and other tissue (blood vessels, collagen; blue). g Segmentation of individual cells (green) based on Nuclear Red staining. h Thresholds for Warp Red and True Blue staining were set to score CD3-CD37dim (blue), CD3+CD37dim (red), CD3+CD37bright (yellow) or CD3-CD37bright (green) cells. i–l Scatter plots showing optical densities for CD3 (Y-axis) and CD37 (X-axis) of individual cells in T cell zones (i, l green), B cell follicles (j, l blue) and red pulp regions (k–l red) and thresholds used for scoring (dotted lines). A representative of 2000 cells per tissue region is plotted

From: de Winde CM, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor CG, van Spriel AB. Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs. Histochem Cell Biol. 2015 May 8. [Epub ahead of print]

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CD3 Antibody | CD3-12 gallery image 7

Published customer image:
Rat anti Mouse CD3 antibody, clone CD3-12 used dor the detection of CD3 expressing cells in human bone marrow, spleen and appendix by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Localization and expression of CD37 in bone marrow (a–f), spleen (g–l) and appendix (m–r). a, g, m Representative original multispectral image of lymphoid organ stained for CD3 (Warp Red), CD37 (True Blue) and cell nuclei (Nuclear Red). Scale bars = 100 µm. b, h, n Composite RGB image after spectral unmixing of original image (red CD3, blue CD37, green nuclei). c, i, o Image showing scoring of CD3-CD37dim (blue), CD3+CD37dim (red), CD3+CD37bright (yellow) or CD3-CD37bright (green) cells. Optical density of CD37 on individual cells in human bone marrow (d), in B cell follicle (blue line) and in T cell zone and in red pulp (red line) in human spleen (j) and in B cell follicle (blue line) and in lamina propria (red line) in human appendix (p). Optical densities were binned per 0.05 and normalized to % of max. Percentage of CD37dim and CD37bright cells in total bone marrow (e) and in the CD3+ cell population in human bone marrow (f). Percentage of total CD37bright cells in B cell follicle and red pulp (k) and in the CD3+ cell population in T cell zone and red pulp (l) in human spleen. Percentage of total CD37bright cells (q) and in the CD3+ cell population (r) in B cell follicle and lamina propria in human appendix. Each dot represents data of one ×20 image from the lymphoid tissue. The red line represents the mean. *P<0.05, ****P<0.0001

From: de Winde CM, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor CG, van Spriel AB.
Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.
Histochem Cell Biol. 2015 May 8. [Epub ahead of print]

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CD3 Antibody | CD3-12 gallery image 8

Published customer image:
Rat anti Human CD3 antibody, clone CD3-12 used for the detection of T lymphocytes in mouse nasal associated lymphoid tissue by immunofluorescence.
Image caption:
Population of 2W:I-Ab tetramer+ cells detected in situ in NALT co-stained with CD3 but not IgG antibodies. (A) Representative image of a whole NALT section from a GAS-2W infected mouse stained with 2W:I-Ab tetramers (red) with one round of TSA amplification, CD3 antibodies (blue), and IgG antibodies (green). For this image, several confocal z-scan fields were collected using a 20X objective and reconstructed as a montage. The enlargement in (B) shows a 2W:I-Ab tetramer-binding T cell that is co-stained with CD3 antibodies but not IgG antibodies. The enlargement in (C) shows 2W:I-Ab tetramer-binding cells that are not co-stained with CD3 or IgG antibodies. (B) and (C) are confocal z-scans collected using the same parameters with a 60X objective and zoom of 3. 2W:I-Ab tetramer+CD3+IgG- cells are indicated with arrowheads, and 2W:I-Ab tetramer+CD3-IgG- cells are indicated with arrows.

From: Dileepan T, Kim HO, Cleary PP, Skinner PJ (2015)
In Situ Peptide-MHC-II Tetramer Staining of Antigen-Specific CD4+ T Cells in Tissues. PLoS ONE 10(6): e0128862.

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CD3 Antibody | CD3-12 gallery image 9

Figure A. Alexa647® conjugated mouse anti human CD8 (MCA1226PE) and RPE conjugated Rat IgG1 isotype control (MCA6004A647). Figure B. Alexa647® conjugated mouse anti human CD8 (MCA1226F) and RPE conjugated rat anti human CD3 (MCA1477A647). All experiments performed on red cell lysed Human peripheral blood gated on leukocytes in the presence of Human SeroBlock (BUF070A).

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  • Rat anti Human CD3:FITC
  • Rat anti Human CD3:Alexa Fluor®647
  • Rat anti Human CD3:FITC
  • Rat anti Human CD3
  • Rat anti Human CD3
  • Rat anti Human CD3:Pacific Blue®
  • Rat anti Human CD3:Alexa Fluor®488
(Rated 0.0 out of 5 based on 0 customer reviews)
    CD3 antibody, clone CD3-12, recognizes human CD3e, and has a very broad species crossreactivity for the CD3 marker. CD3 is a multimeric protein complex composed of four distinct polypeptide chains that associate with the T cell receptor (TCR).
    • Product Type
      Monoclonal Antibody
    • Clone
      CD3-12
    • Isotype
      IgG1
    5 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA1477FF *datasheet pdfdatasheet pdf0.1 mg
      MCA1477F
      MCA1477C, F *, IF, P *, WBdatasheet pdfdatasheet pdf0.2 mg
      MCA1477
      MCA1477A647F *datasheet pdfdatasheet pdf100 Tests/1ml
      MCA1477A647
      MCA1477PBF *datasheet pdfdatasheet pdf100 Tests/1ml
      MCA1477PB
      MCA1477A488F *datasheet pdfdatasheet pdf100 Tests/1ml
      MCA1477A488
      MCA1477FTF *datasheet pdfdatasheet pdf25 µg
      MCA1477FT
      MCA1477TC, F *, IF, P *datasheet pdfdatasheet pdf25 µg
      MCA1477T
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Rat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3ε chain recognizes human CD3ε. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker.

        As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005)
      • Intended Use
      • Target Species
        Human
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Bovineyes
        Dogyes
        Horseyes
        Rhesus Monkeyyes
        Pigyes
        Chickenyes
        Raccoonyes
        Great horned owl (Bubo virginianus)yes
        Bullfrogyes
        Xenopusyes
        AmphibiaExpected from Sequence
        Rabbityes
        Spotted Hyenayes
        Sea Lionyes
        Catyes
        Amazon Parrotyes
        MammalsExpected from Sequence
        BirdsExpected from Sequence
        Mouseyes
        Duckyes
        Koalayes
        Harbour Porpoiseyes
        Alpacayes
        Cynomolgus monkeyyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG conjugated to Alexa Fluor 647 - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG conjugated to Pacific Blue - liquid
        Purified IgG conjugated to Alexa Fluor 488 - liquid
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Pack Size: 25 µgPurified IgG prepared from tissue culture supernatant
        Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Pack Size: 25 µgPurified IgG prepared from tissue culture supernatant
        Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      • Preservative Stabilisers
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        0.09%Sodium Azide
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
      • Immunogen
        Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC )
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 0.1 mg/ml
        IgG concentration 0.05mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
      • GO Terms
        receptor signaling complex scaffold activity
        signal complex assembly
        protein kinase binding
        external side of plasma membrane
        T cell receptor signaling pathway
        T cell receptor binding
        transmembrane receptor activity
        receptor signaling protein activity
        integral to plasma membrane
        protein heterodimerization activity
        G-protein coupled receptor protein signaling pathway
        SH3 domain binding
        T cell costimulation
      • UniProt
      • Entrez Gene
      • Acknowledgements
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)1/51/10
        (1)
        Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat
        (1)
        Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)1/51/10
        (1)
        Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Immunofluorescence
        Immunohistology - Frozen1/100
        Western Blotting
        Flow Cytometry(1)1/501/100
        Immunohistology - Paraffin(2)1/100
        (1)
        Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
        (2)
        This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Immunofluorescence
        Immunohistology - Frozen1/100
        Western Blotting
        Flow Cytometry(1)1/501/100
        Immunohistology - Paraffin(2)1/100
        (1)
        Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
        (2)
        This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)1/51/10
        (1)
        Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)1/51/10
        (1)
        Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm™ (BUF09) for this purpose.

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
        Tonsil
      • Histology Positive Control Tissue
        Tonsil
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use

      Additional CD3 Antibody Formats

      Formats Clone Applications Sizes available
      CD3 Antibody : Purified CD3-12 C, F *, IF, P *, WB 0.2 mg | 25 µg
      CD3 Antibody : FITC CD3-12 F * 0.1 mg | 25 µg
      CD3 Antibody : Pacific Blue® CD3-12 F * 100 Tests/1ml
      CD3 Antibody : Alexa Fluor® 647 CD3-12 F * 100 Tests/1ml
      CD3 Antibody : Alexa Fluor® 488 CD3-12 F * 100 Tests/1ml
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
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        Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
        STAR131B
        Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
        STAR71D549GA
        Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
        STAR71D649GA
        Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
        STAR16D800GA
        Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
        STAR71D800GA
        Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
        STAR69
        Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
        STAR17B
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72
        Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
        STAR21B
        Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
        STAR73
        Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
        STAR20A
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
        STAR131A
        Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
        STAR131B
        Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
        STAR71D549GA
        Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
        STAR71D649GA
        Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
        STAR16D800GA
        Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
        STAR71D800GA
        Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
        STAR69
        Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
        STAR17B
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72
        Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
        STAR21B
        Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
        STAR73
        Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
        STAR20A

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG1 Negative Control:FITCMCA6004F0.1 mgF
          MCA6004F
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG1 Negative Control:Alexa Fluor® 647MCA6004A647100 Tests/1mlF
          MCA6004A647
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG1 Negative Control:FITCMCA6004F0.1 mgF
          MCA6004F
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG1 Negative ControlMCA6004GA0.1 mgF
          MCA6004GA
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG1 Negative ControlMCA6004GA0.1 mgF
          MCA6004GA
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG1 Negative Control:Pacific Blue®MCA6004PB100 Tests/1mlF
          MCA6004PB
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG1 Negative Control:Alexa Fluor® 488MCA6004A488100 Tests/1mlF
          MCA6004A488

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human SeroblockBUF070B200 TestF
            BUF070B
            Human SeroblockBUF070A50 TestF
            BUF070A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human SeroblockBUF070B200 TestF
            BUF070B
            Human SeroblockBUF070A50 TestF
            BUF070A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human SeroblockBUF070B200 TestF
            BUF070B
            Human SeroblockBUF070A50 TestF
            BUF070A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human SeroblockBUF070B200 TestF
            BUF070B
            Human SeroblockBUF070A50 TestF
            BUF070A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human SeroblockBUF070B200 TestF
            BUF070B
            Human SeroblockBUF070A50 TestF
            BUF070A

            Recommended Positive Controls

              Histology Controls

                Tonsil
                Tonsil

                References

                Further Reading

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