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Rat IgG1 Negative Control antibody

Rat IgG1 Negative Control

Product Type
Negative/Isotype Control
Isotype
IgG1
Specificity
Rat IgG1 Negative Control

Product Code Applications Pack Size List Price Your Price Qty
MCA6004GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
MCA6004
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 1 mg loader
List Price Your Price
loader

Rat IgG1 negative control, a rat monoclonal raised against horseradish peroxidase, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG1 test antibodies to the surface of human and mouse cells in flow cytometry.

Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
Isotype controls savings up to 65%

Target Species
Negative Control
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Horseradish peroxidase
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

References for Rat IgG1 Negative Control antibody

  1. do Prado Duzanski, A. et al. (2022) Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?
    Research in Veterinary Science. 145: 193-204.
  2. Matralis, D.T. et al. (2023) Intracellular IFN-γ and IL-4 levels of CD4 + and CD8 + T cells in the peripheral blood of naturally infected (Leishmania infantum) symptomatic dogs before and following a 4-week treatment with miltefosine and allopurinol: a double-blinded, controlled and cross-sectional study.
    Acta Vet Scand. 65 (1): 2.

MCA6004GA

0615 164012

MCA6004

0215 156348

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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