CD222 Antibody | MEM-238

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CD222 Antibody | MEM-238 gallery image 1

Staining of permeablised human peripheral blood granulocytes with Mouse anti Human CD222 (MCA2048T)

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CD222 Antibody | MEM-238 gallery image 2

Staining of permeablised human peripheral blood monocytes with Mouse anti Human CD222:FITC (MCA2048F)

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CD222 Antibody | MEM-238 gallery image 3

Staining of permeablised human peripheral blood granulocytes with Mouse anti Human CD222:APC (MCA2048APC)

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  • CD222 Antibody | MEM-238 thumbnail image 1
  • CD222 Antibody | MEM-238 thumbnail image 2
  • CD222 Antibody | MEM-238 thumbnail image 3
  • Mouse anti Human CD222:APC
  • Mouse anti Human CD222
  • Mouse anti Human CD222
  • Mouse anti Human CD222:FITC
  • Mouse anti Human CD222:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
    MEM-238
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2048FF *datasheet pdfdatasheet pdf0.1 mg
    MCA2048F
    MCA2048F *, IFdatasheet pdfdatasheet pdf0.2 mg
    MCA2048
    MCA2048APCF *datasheet pdfdatasheet pdf100 Tests
    MCA2048APC
    MCA2048TF *datasheet pdfdatasheet pdf25 µg
    MCA2048T
    MCA2048FTF *datasheet pdfdatasheet pdf25 µg
    MCA2048FT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD222 antibody, clone MEM-238 recognizes human CD222, a 250 kDa transmembrane protein originally identified as the IGF II receptor. CD222 is ubiquitously expressed and is involved with internalization of a variety of ligands.

      Mouse anti Human CD222 antibody, clone MEM-238 recognizes an epitope located in the region between extracellular domains 2 and 5 (aa 192-697) of CD222 (Roberts et al. 2010).

      CD222 is primarily expressed intracellularly with a small percentage of molecules being located at the cell surface (5 - 10%).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Rhesus Monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Recombinant vaccinia virus containing CD222.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4ºC or at -20ºC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4ºC or at -20ºC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4ºC or at -20ºC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4ºC or at -20ºC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      cell surface
      endosome
      transporter activity
      trans-Golgi network transport vesicle
      glycoprotein binding
      integral to plasma membrane
      receptor-mediated endocytosis
      insulin-like growth factor receptor activity
      phosphoprotein binding
      lysosomal membrane
      endocytic vesicle
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)neat
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Flow Cytometry(1)1/251/50
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Flow Cytometry(1)1/251/50
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)neat
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)neat
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD222 Antibody Formats

    Formats Clone Applications Sizes available
    CD222 Antibody : Purified MEM-238 F *, IF 0.2 mg | 25 µg
    CD222 Antibody : APC MEM-238 F * 100 Tests
    CD222 Antibody : FITC MEM-238 F * 25 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
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      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
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      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:APCMCA928APC100 TestsF
        MCA928APC
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
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          Human SeroblockBUF070A50 TestF
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          Human SeroblockBUF070A50 TestF
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry
                Immunofluorescence

              References

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