CD105 Antibody | SN6

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CD105 Antibody | SN6 gallery image 1

Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor® 488 (MCA1557A488)

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CD105 Antibody | SN6 gallery image 2

Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor® 647 (MCA1557A647)

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CD105 Antibody | SN6 gallery image 3

Staining of KG1 cells with Mouse anti Human CD105 (MCA1557)

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CD105 Antibody | SN6 gallery image 4

Staining of KG1 cells with Mouse anti Human CD105:FITC (MCA1557F)

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CD105 Antibody | SN6 gallery image 5

Staining of KG1 cells with Mouse anti Human CD105RPE (MCA1557PE)

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CD105 Antibody | SN6 gallery image 6

Published customer image:
AlexaFlour®488 conjugated Mouse anti Human CD105 antibody, clone SN6 (MCA1557A488) used to evaluate endoglin expression on equine, tendon derived stem/progenitor cells by flow cytometry.
Image caption:
Flow cytometry analysis of cell surface markers CD90, CD73, and CD105 on tendon-derived stem/progenitor cells isolated by differential adhesion onto substrates precoated with 20?μg/ml fibronectin (f-TSPCs) and grown in normoxia and 5% hypoxia. A–C: f-TSPCs (age 1 year) grown in 21% oxygen and incubated with control or antibodies (+) to CD90 (A), CD73 (B), or CD105 (C) for flow cytometry. D–F: f-TSPCs grown in 5% oxygen and incubated with control or antibodies (+) to CD90 (D), CD73 (E), or CD105 (F) for flow cytometry. a p?=?0.024, b p?=?0.04, c p?=?0.038.

From: Williamson KA, Lee KJ, Humphreys WJ, Comerford EJ, Clegg PD, Canty-Laird EG.
Restricted differentiation potential of progenitor cell populations obtained from the equine superficial digital flexor tendon (SDFT).
J Orthop Res. 2015 Jun;33(6):849-58.

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CD105 Antibody | SN6 gallery image 7

Published customer image:
Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.
Image caption:
Endothelial adherence of blood monocytes. PBMCs were isolated from HLA-A2+ donors and incubated with HLA-A2- HUVECs (1×106 cells/well) for 2 h, after which the non-adherent PBMCs were removed by washing. The co-cultured cell layers were immediately analysed with dual-colour flow cytometry for HLA-A2 and (A) CD34, (B) CD14, (C) CD11b, (D) CD16, (E) CD105 and (F) CD144 expression. Representative plots from 4–6 individual experiments are shown. (G) Two parameters dot plot showing typical isotype controls.

From: Tso C, Rye K-A, Barter P (2012)
Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium.
PLoS ONE 7(5): e37091.

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CD105 Antibody | SN6 gallery image 8

Published customer image:
Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.
Image caption:
Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1×106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.

From: Tso C, Rye K-A, Barter P (2012)
Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium.
PLoS ONE 7(5): e37091.

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CD105 Antibody | SN6 gallery image 9

Published customer image:
Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.
Image caption:
Increased expression of CD105 and CD144 in the endothelium-adherent monocytes during co-culture. HLA-A2+ PBMCs (1×106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were maintained in co-culture up to Day 6, then assessed by dual-colour flow cytometry for HLA-A2 and (A) CD105 and (B) CD144 expression on Day 3 of co-culture. Representative plots from 4–7 individual experiments are shown. The increase in CD105 from Day 0 to Day 6 (C) and CD144 expression from Day 0 to Day 6 (D) is also shown.

From: Tso C, Rye K-A, Barter P (2012)
Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium.
PLoS ONE 7(5): e37091.

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CD105 Antibody | SN6 gallery image 10

Published customer image:
Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.
Image caption:
Expression of endothelial antigens in endothelium-adherent monocytes in co-culture with HCAECs. HLA-A2+ PBMCs (1×106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HCAECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by dual-colour flow cytometry for HLA-A2 and (A) CD105, (B) eNOS and (C) VEGFR2 expression on Day 2 of co-culture.

From: Tso C, Rye K-A, Barter P (2012)
Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium.
PLoS ONE 7(5): e37091.

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CD105 Antibody | SN6 gallery image 11

Published customer image:
Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry
Image caption:
Immunophenotype of mesenchymal stem cells from human bone marrow. MSC cells were prepared as reported in Materials and Methods. MSCs from passage 2 were harvested and labeled with antibodies against CD105 and CD29 (positive MSCs markers) and CD45, CD34 and CD14 (MSCs negative markers) and analyzed by FACS. Histograms represent the staining of cells with the indicated antibody.

From: Borriello A, Caldarelli I, Basile MA, Bencivenga D, Tramontano A, et al.
(2011) The Tyrosine Kinase Inhibitor Dasatinib Induces a Marked Adipogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells.
PLoS ONE 6(12): e28555.

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CD105 Antibody | SN6 gallery image 12

Published customer image:
AlexaFlourA488® conjugated Mouse anti Human CD105 antibody used for flow cytometry on human mesenchymal stem cells.
Image caption:
Immunophenotyping of freshly isolated ((c), (d)) and cultured CD105+ hMSCs (d) was evaluated by flow cytometry after staining of specific cell surface markers. Corresponding isotype controls were used as negative controls

From: Anna Schade, Paula Müller, Evgenya Delyagina, et al.
Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs,”
Stem Cells International, vol. 2014, Article ID 197154, 11 pages.

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CD105 Antibody | SN6 gallery image 13

Published customer image:
FITC conjugated Mouse anti Human CD105 antibody used for flow cytometry on human bone marrow deived mesenchymal stem cells.
Image caption:
Flow cytometric analysis and Wnt profiles of hMSCs by the induction of NTs. (A) Bone marrow-derived hMSCs were analyzed following four cell passages. hMSCs were positive for CD44, CD73, CD105, CD166, and Stro-1, and negative for CD14 and CD34. The solid curves indicate each type of antibody, and the filled curves indicate mouse IgG as the negative control. (B) mRNA levels of MAP2 were quantified on days 7, 14, and 21 during stimulation with NTs. NTs significantly increased MAP2 levels on days 14 and 21. Untreated hMSCs served as the control. (C) mRNA levels of Wnt1, Wnt3a, Wnt5a, Wnt7a, and Wnt7b were quantified on days 7, 14, and 21 during stimulation with NTs. NTs increased the expression of Wnt1 and induced expressions of Wnt7a and Wnt7b. * p<0.05, ** p<0.01 (i.e., treated vs. control in the Wnt1, Wnt3a, and Wnt5a groups; NTs at 14 and 21 days vs. NTs at 7 days in the Wnt7a and Wnt7b groups). Data are presented as the mean ± SD of one triplicate experiment that was representative of three independent experiments. * p<0.05, ** p<0.01 (i.e., treated vs. control). ND, not determined.

From: Tsai H-L, Deng W-P, Lai W-FT, Chiu W-T, Yang C-B, et al. (2014)
Wnts Enhance Neurotrophin-Induced Neuronal Differentiation in Adult Bone-Marrow-Derived Mesenchymal Stem Cells via Canonical and Noncanonical Signaling Pathways.
PLoS ONE 9(8): e104937.

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CD105 Antibody | SN6 gallery image 14

Published customer image:
Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry on brain microvascular endothelial cells
Image caption:
Characterization and functional features of human and murine BMVECs. (A) Phase contrast micrographs of confluent monolayers of human (left image) and murine (right image) BMVECs. BMVECs present the typical “cobblestone appearance”. Scale bar, 100 μm and 200 μm for human BMVECs and murine BMVECs. B) Human (left image) and murine (right image) BMVECs showed a clear cytoplasmic staining for CD31. Scale bar, 50 μm. C) Human (left image) and murine (right image) cells displayed an intense positive immunofluorescence for vWf. Scale bar, 50 μm. D) Flow cytometric analysis of BMVECs. Human BMVECs resulted positive (gray histograms) for CD31 (left graph), CD105, CD146 (left gaph), UEA-1 staining; murine BMVECs resulted positive for CD31 (right graph), CD34, CD146 (right graph) and Tie-2 staining. White histograms represent the isotype controls of each antibody. E) Capillary tube-like structure produced by human (left image) and murine (right image) BMVECs, 7 h after plating onto Matrigel. Scale bar, 100 μm. F) LDL-uptake assay on human (left image) and murine (right image) BMVECs. Scale bar, 50 μm. G) Human (left image) and murine (right image) BMVECs were labelled for GLUT-1. Scale bar, 50 μm. H) Immunofluorescence for eNOS in human (left image) and murine (right image) BMVECs. Scale bar, 50 μm. All nuclei were counterstained with DAPI (blue). One representative of three independent experiments performed in blind is shown for each figure.

From: Navone SE, Marfia G, Nava S, Invernici G, Cristini S, Balbi S, Sangiorgi S, Ciusani E, Bosutti A, Alessandri G, Slevin M, Parati EA.
Human and mouse brain-derived endothelial cells require high levels of growth factors medium for their isolation, in vitro maintenance and survival.
Vasc Cell. 2013 May 14;5(1):10.

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Published customer image:
FITC conjugated Mouse anti Human CD105 antibody, clone SN6 (MCA1557F) used for the evaluation of endoglin expression on mesenchymal stem cells by flow cytometry.
Image caption:
(A) Platinum accumulation in MSC and TGCT cell lines upon 24h treatment with 3 μM cisplatin and analyzed by atomic absorption spectroscopy. Mean ± standard deviation; MSC n = 8, TGCT both n = 3; * p<0.05 vs MSC. (B) Cell cycle populations from analyses as shown in Fig 1C (propidium iodide staining). Data are presented as% of cells distributed to cell cycle phases as mean ± standard deviation; n≥4; * p<0.05, *** p<0.001 vs. control. (C) MSC after subapoptotic damage by cisplatin upon reconstitution of proliferation were analyzed for surface antigen expression by flow cytometry. Data are shown as histograms of fluorescence. Isotype controls (no filling) are overlaid on specific FITC- or PE-conjugated antibodies. Data are representative of at least 4 independent experiments. (D) MSC from (C) were incubated in growth medium (u) or specific osteogenic (o) and adipogenic (a) differentiation media. Cells were stained with alizarin pH4 and oil red for calcium deposition and lipid droplets, respectively. Data are representative of at least 4 independent experiments. Light microscopy, scale bar– 100μm.

From: Lützkendorf J, Wieduwild E, Nerger K, Lambrecht N, Schmoll H-J, Müller-Tidow C, et al. (2017)
Resistance for Genotoxic Damage in Mesenchymal Stromal Cells Is Increased by Hypoxia but Not Generally Dependent on p53-Regulated Cell Cycle Arrest.
PLoS ONE 12(1): e0169921.

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CD105 Antibody | SN6 gallery image 16

Published customer image:
FITC conjugated Mouse anti Human CD105 antibody, clone SN6 (MCA1557F) used for the evaluation of endoglin expression on mesenchymal stem cells by flow cytometry.
Image caption:
(A) MSC cultured for up to 14 days under physioxia or hypoxia were analyzed for surface antigen expression by flow cytometry. Data are representative of at least 3 independent experiments. (B) MSC from (A) were incubated in growth medium (u) or specific osteogenic (o) and adipogenic (a) differentiation media. Cells were stained with alizarin pH4 and oil red for calcium deposition and lipid droplets, respectively. Data are representative of 3 independent experiments. Light microscopy, scale bar– 100 μm. (C) Growth kinetics of MSC under normoxic, physioxic and hypoxic conditions. Cultivation under physioxia/hypoxia started on day 0. An aliquot of hypoxic cells was reoxygenated to normoxic conditions on d15. Data are representative of 5 independent experiments. (D) Cell cycle analyses of normoxic and hypoxic MSC were performed upon pyronin/7-AAD staining. Data are presented as% of cells in cell cycle phase as mean—standard deviation; n = 5.

From: Lützkendorf J, Wieduwild E, Nerger K, Lambrecht N, Schmoll H-J, Müller-Tidow C, et al. (2017)
Resistance for Genotoxic Damage in Mesenchymal Stromal Cells Is Increased by Hypoxia but Not Generally Dependent on p53-Regulated Cell Cycle Arrest.
PLoS ONE 12(1): e0169921.

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CD105 Antibody | SN6 gallery image 17

Published customer image:
FITC conjugated Mouse anti Human CD105 antibody, clone SN6 (MCA1557F) used for the evaluation of endoglin expression on mesenchymal stem cells by flow cytometry.
Image caption:
(A) Growth kinetic was performed with MSC with lentiviral p53 knock down (MSCp53kd), MSC with lentiviral control sh-RNA (ctr-MSC) and wildtype MSC (wt-MSC) from the same donor. Lentiviral transduction was performed on day 0. Data are representative of 4 independent experiments. (B) Late passage MSCp53kd were stained for senescence-associated beta-galactosidase activity. Data are representative of 2 independent experiments. Light microscopy, scale bar– 200 μm. (C) MSCp53kd were analyzed for surface antigen expression by flow cytometry. Data are shown as histograms of fluorescence. Isotype controls (no filling) are overlaid on specific FITC- or PE-conjugated antibodies. Data are representative of 4 independent experiments. (D) MSCp53kd were incubated in growth medium (u) or specific osteogenic (o) and adipogenic (a) differentiation media. Cells were stained with alizarin pH4 and oil red for calcium deposition and lipid droplets, respectively. Data are representative of 4 independent experiments. Light microscopy, scale bar– 200 μm. (E) MSCp53kd were treated 72 h with cisplatin under normoxic, physioxic and hypoxic conditions and analyzed for cell cycle distribution. Data are presented as% of cells in cell cycle phase as mean—standard deviation; n = 3. (F) Whole protein lysates from the experiment shown in (E) were analyzed by western blot. Data are representative of 3 independent experiments.

From: Lützkendorf J, Wieduwild E, Nerger K, Lambrecht N, Schmoll H-J, Müller-Tidow C, et al. (2017)
Resistance for Genotoxic Damage in Mesenchymal Stromal Cells Is Increased by Hypoxia but Not Generally Dependent on p53-Regulated Cell Cycle Arrest.
PLoS ONE 12(1): e0169921.

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CD105 Antibody | SN6 gallery image 18

Published customer image:
Mouse anti Human CD105 antibody, clone SN6 (MCA1557) used for the identification of mesenchymal stem cells in mixed bone marrow cell populations by flow cytometry.
Image caption:
MSCs used in this study were characterized for marker expression by flow cytometric analysis. Dashed histograms indicate staining with isotype-matched control antibody and solid histograms denote the specific expression of each indicated marker.

From: Lee HJ, Kim SN, Jeon MS, Yi T, Song SU.
ICOSL expression in human bone marrow-derived mesenchymal stem cells promotes induction of regulatory T cells.
Sci Rep. 2017 Mar 14;7:44486.

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  • Mouse anti Human CD105:Alexa Fluor® 488
  • Mouse anti Human CD105:Alexa Fluor® 647
  • Mouse anti Human CD105:RPE
  • Mouse anti Human CD105
  • Mouse anti Human CD105:FITC
  • Mouse anti Human CD105
  • Mouse anti Human CD105:Alexa Fluor® 647
  • Mouse anti Human CD105:Alexa Fluor® 488
  • Mouse anti Human CD105:RPE
  • Mouse anti Human CD105:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    SN6
  • Isotype
    IgG1
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1557FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1557F
    MCA1557C *, F, IP, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA1557
    MCA1557PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1557PE
    MCA1557A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1557A488
    MCA1557A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1557A647
    MCA1557FTFdatasheet pdfdatasheet pdf25 µg
    MCA1557FT
    MCA1557TC *, F, IP, WBdatasheet pdfdatasheet pdf25 µg
    MCA1557T
    MCA1557PETFdatasheet pdfdatasheet pdf25 Tests
    MCA1557PET
    MCA1557A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA1557A647T
    MCA1557A488TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA1557A488T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD105 antibody, clone SN6 recognizes the human endoglin, also known as CD105. CD105 is a glycoprotein homodimer of 95 kDa subunits expressed by endothelial cells, activated monocytes and some leukemia cells.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Horseyes
      Cynomolgus monkeyyes
      Rhesus Monkeyyes
      PrimateExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG conjugated to Alexa Fluor® 647- liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647- liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1ml distilled water
      Pack Size: 25 TestsReconstitute with 0.25 ml distilled water
      Pack Size: 100 TestsReconstitute with 1ml distilled water
      Pack Size: 25 TestsReconstitute with 0.25 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Partially purified cell membrane antigens from fresh leukemia cells
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 TestsStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 TestsStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      membrane fraction
      negative regulation of nitric-oxide synthase activity
      regulation of cell proliferation
      transforming growth factor beta receptor, cytoplasmic mediator activity
      negative regulation of transforming growth factor beta receptor signaling pathway
      type II transforming growth factor beta receptor binding
      positive regulation of BMP signaling pathway
      transforming growth factor beta binding
      transforming growth factor beta receptor signaling pathway
      regulation of cell adhesion
      type I transforming growth factor beta receptor binding
      BMP signaling pathway
      protein homodimerization activity
      transforming growth factor beta receptor complex
      extracellular matrix disassembly
      regulation of transforming growth factor beta receptor signaling pathway
      galactose binding
      positive regulation of systemic arterial blood pressure
      detection of hypoxia
      extracellular space
      positive regulation of pathway-restricted SMAD protein phosphorylation
      external side of plasma membrane
      negative regulation of gene-specific transcription from RNA polymerase II promoter
      wound healing
      smooth muscle tissue development
      negative regulation of pathway-restricted SMAD protein phosphorylation
      glycosaminoglycan binding
      cell chemotaxis
      negative regulation of endothelial cell proliferation
      regulation of transcription
      heart looping
      cell surface
      activin binding
      cell adhesion
      venous blood vessel morphogenesis
      central nervous system vasculogenesis
      chronological cell aging
      transforming growth factor beta receptor activity
      transmembrane receptor activity
      negative regulation of protein autophosphorylation
      artery morphogenesis
      positive regulation of gene-specific transcription from RNA polymerase II promoter
      patterning of blood vessels
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD105 Antibody Formats

    Formats Clone Applications Sizes available
    CD105 Antibody : Purified SN6 C *, F, IP, WB 0.2 mg | 25 µg
    CD105 Antibody : FITC SN6 F 0.1 mg | 25 µg
    CD105 Antibody : RPE SN6 F 25 Tests | 100 Tests
    CD105 Antibody : Alexa Fluor® 647 SN6 F 25 Tests/0.25ml | 100 Tests/1ml
    CD105 Antibody : Alexa Fluor® 488 SN6 F 100 Tests/1ml | 25 Tests/0.25ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
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      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
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      STAR117D680GA
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      STAR117D800GA
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      STAR8D800GA
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      STAR117F
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      STAR70
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      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      STAR87A
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      STAR117D488GA
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      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
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      STAR117D680GA
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      STAR117D800GA
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      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
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      STAR120F
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      STAR9B
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      HCA036P
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      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
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      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
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      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 488MCA928A488100 Tests/1mlF
        MCA928A488
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647100 Tests/1mlF
        MCA928A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA928PE100 TestsF
        MCA928PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647100 Tests/1mlF
        MCA928A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 488MCA928A488100 Tests/1mlF
        MCA928A488
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA928PE100 TestsF
        MCA928PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
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        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
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          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Human SeroblockBUF070A50 TestF
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          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

              Further Reading

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