C4d Antibody | 10-11

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C4d Antibody | 10-11 gallery image 1

Immunohistochemical analysis of formalin fixed paraffin embedded human renal allograft tissue. Tissue was incubated with mouse anti human C4d (2222-8004) following heat mediated antigen retrieval.

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  • Mouse anti Human C4d
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    Mouse anti Human C4d antibody, clone 10-11 recognizes complement component 4d. The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection. C4d is a footprint of complement activation, making it a perfect maker of allograft rejection.
    • Product Type
      Monoclonal Antibody
    • Clone
      10-11
    • Isotype
      IgG1
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      2222-8004C, E, IF, P *, WBdatasheet pdfdatasheet pdf0.1 mg
      2222-8004
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
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      • Mouse anti Human C4d antibody, clone 10-11 recognizes the secreted protein complement component 4d (C4d). The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection [AMR] (Collins et al. 1999.).

        C4d was accepted in 2003 into the Banff classification for identification of acute AMR (Racusen et al. 2003). Mouse anti Human C4d antibody, clone 10-11 is specific for C4d, a marker that can be used in the detection of acute AMR for kidney, heart, pancreas and lung allografts. C4d is regarded as a key marker of antibody-mediated cell injury and humoral rejection (Sacks and Chowdhury 2002).

        Complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4b’s short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation (Collins et al. 1999).

        Mouse anti Human C4d antibody, clone 10-11 is used to detect the biomarker C4d which has been described as a “footprint” of antibody mediated tissue rejection (Sacks and Chowdhury 2002). The internal thioester of C4b becomes exposed during cleavage to C4d and forms a covalent bond with the cell surface. The longer half-life of covalently bound C4d makes it a footprint of complement activation long after weakly bound antibodies have been cleared by the blood stream (Sacks and Chowdhury 2002).

        C4 has also been linked to susceptibility to systemic lupus erythematosus (Yang et al. 2004) and rheumatoid arthritis (Makinde et al. 1989).
      • Intended Use
      • Target Species
        Human
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseno
        Dogno
        Bovineno
        Catno
        Rabbitno
        Ratno
        Guinea Pigno
        Sheepno
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.09%Sodium Azide (NaN3)
      • Immunogen
        Native, from human plasma
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 1.0 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Borate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted.
        Avoid repeated freezing and thawing as this may denature the antibody.
        Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
      • UniProt
      • Entrez Gene
      • Acknowledgements
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA1/50001/20000
        Immunofluorescence1/2501/600
        Immunohistology - Frozen1/1001/750
        Western Blotting
        Immunohistology - Paraffin(1)
        (1)
        It has been reported that this antibody works very well on acetone-fixed, frozen renal biopsies. Strong staining is observed in the glomeruli and in some cases the peritubular capillaries.

        Clone 10-11 has given variable results on formalin-fixed, paraffin-embedded sections. It has been observed that pre-treatment with 88% formic acid for 20 minutes at room temperature is beneficial (6).

        For best results Bio-Rad recommend HISTAR detection kits (STAR3000A/B/C).

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
      • Technical Advice
      • Recommended Protocol
      • ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional C4d Antibody Formats

      Formats Clone Applications Sizes available
      C4d Antibody : Purified 10-11 C, E, IF, P *, WB 0.1 mg
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                • Application NameReference Images
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                References

                Further Reading

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