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Lymphocytes Antibody | MsGp2

Lymphocytes Antibody | MsGp2 gallery image 1

Published customer image:
Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 used for the detection of infiltrating lymphocytes in guinea pig skin following intradermal Mantoux injection and subsequent electroporation by immunofluorescence.
Image caption:
Histological analysis reveals infiltration of lymphocytes at the EP treatment site. Guinea pig skin sections were immunohistochemically stained with an antibody recognizing lymphocytes and Langerhans cells (Tetramethylrhodamine isothiocyanate (TRITC) secondary) after ID GFP plasmid administration followed by EP with SEP. Skin biopsies were removed, cryosectioned, antibody and 4',6-diamidino-2-phenylindole (DAPI) stained and visualized under fluorescence microscopy at 20× and 40×. An untreated control (no DNA, no EP) is also shown.

From: Mendoza, J.M.; Amante, D.H.; Kichaev, G.; Knott, C.L.; Kiosses, W.B.; Smith, T.R.F.; Sardesai, N.Y.; Broderick, K.E.
Elucidating the Kinetics of Expression and Immune Cell Infiltration Resulting from Plasmid Gene Delivery Enhanced by Surface Dermal Electroporation. Vaccines 2013, 1, 384-397.

Lymphocytes Antibody | MsGp2 gallery image 2

Staining of Guinea Pig peripheral blood lymphocytes with Mouse anti Guinea Pig Lymphocytes and Langerhans Cells (MCA560GA) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F)

  • Lymphocytes Antibody | MsGp2 thumbnail image 1
  • Lymphocytes Antibody | MsGp2 thumbnail image 2
  • Mouse anti Guinea Pig Lymphocytes and Langerhans Cells
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA560GAC, Fdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 recognizes an antigen present on the majority of T and B lymphocytes and thymocytes, although the antigen is not expressed by germinal centre cells of the lymph node (Kraal et al 1986).

      Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 also reacts strongly with epidermal Langerhans cells, giving a superior profile than anti-MHC Class II antibodies on these cells (Healey et al. 1987).

      Clone MsGp2 has the same distribution as Mouse anti Guinea Pig CD90 antibody, clone CT4 which detects an antigen reportedly involved in the organ non-specific trafficking of guinea pig lymphocytes (Kraal et al 1988). However, unlike clone CT4, clone MsGp2 does not functionally inhibit lymphocyte adherence to high endothelial venules in vitro (Healey et al. 1987).
    • Intended Use
    • Target Species
      Guinea Pig
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Nylon wool adherent B cells
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunohistology - Frozen

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Lymphocytes Antibody Formats

    Formats Clone Applications Sizes available
    Lymphocytes Antibody : Purified MsGp2 C, F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Guinea Pig T LymphocytesMCA564GA0.1 mgC, F

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Lymphocytes Antibody | MsGp2 thumbnail image 1
                • Lymphocytes Antibody | MsGp2 thumbnail image 2
              • Application NameReference Images
                Immunohistology - Frozen


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