Recombinant Bovine GM-CSF detected using Mouse anti Bovine GM-CSF (MCA1828GA) as the capture reagent and a bovine reactive Mouse anti Sheep GM-CSF, clone 3C2 (MCA1924) conjugated to Biotin as the detection reagent followed by Streptavidin:HRP
Mouse anti Bovine GM-CSF antibody, clone CC305 recognizes bovine granulocyte macrophage colony-stimulating factor (GM-CSF) also known as CSF-2. Bovine GM-CSF is a 143 amino acid cytokine which stimulates proliferation and differentiation of hematopoietic precursors.
Mouse anti Bovine GM-CSF antibody, clone CC305 has been used as a capture antibody in conjunction with Mouse anti Sheep GM-CSF, clone 3C2 as a detection reagent in a sandwich ELISA for the measurement of bovine GM-CSF produced by peripheral blood mononuclear cells in vitro (Kennedy et al. 2002).
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Spleen cells from immunised C57/BL mice were fused with cells of the mouse SP2/0 myeloma cell line.
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
5 ug/ml as coating antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
This product may be used as a capture antibody in a sandwich ELISA together with MCA1924 when conjugated to biotin as the detection reagent.