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CD8 Antibody | CC63

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CD8 Antibody | CC63 gallery image 1

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD8: FITC (MCA837F)

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CD8 Antibody | CC63 gallery image 2

Bovine lymph node stained with Mouse anti Bovine CD8 (MCA837GA) 10x. Image courtesy of Dr. J.B. Silvan, Moredun Research Institute, Roslin, UK

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CD8 Antibody | CC63 gallery image 3

Bovine lymph node stained with Mouse anti Bovine CD8 (MCA837GA) 40x. Image courtesy of Dr. J.B. Silvan, Moredun Research Institute, Roslin, UK

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CD8 Antibody | CC63 gallery image 4

Published customer image:
Mouse anti Bovine CD8 antibody, clone CC63 used for the identification of CD8 expressing cells inthe nasopharynx by immunofluorescence.
Image caption:
Nasopharyngeal primary infection sites of vaccinated cattle are infiltrated by CD8+/CD3- (presumptive NK) cells at 24 hpi.
Multichannel immunofluorescent technique. A). FMDV VP1 (red) protein within cytokeratin+ cells (green) in epithelial crypt of the nasopharyngeal mucosa of non-vaccinated steer at 24 hpi (animal number 3). CD8+ (aqua)/ CD3+ (purple) double-positive CTLs are present in the subepithelial compartment amongst larger populations of CD8-/CD3+ T-lymphocytes. 20x magnification, scale bar 50μm. B) 40x magnification of region identified in (A), demonstrating consistent co-localization of CD8 (aqua) with CD3 (purple). Scale bars 25μm. B) Select channels of image shown in (B). CD3+ cells (purple) include single-positive (T-lymphocytes) or CD8+/CD3+ double-positive (CTLs). CD8 (aqua) is exclusively detected in combination with CD3 (purple). Scale bars 25 μm. D) FMDV VP1 (red) protein co-localize with cytokeratin (green) in a focal surface erosion within follicle-associated epithelium of nasopharyngeal mucosa of vaccinated steer at 24 hpi (animal number 11). A distinct population of cells defined as presumptive NK-cells based on a CD8+ (aqua)/CD3- (purple) phenotype is present in submucosal and epithelial compartments surrounding the focus of infection. A smaller population of CTLs (CD8+/CD3+) is present amongst abundant non-CTL T-lymphocytes (CD8-/CD3+). 20x magnification, scale bar 50μm. E) Higher magnification of region identified in showing CD8+/CD3- cells (aqua) representing presumptive NK cells in close proximity of FMDV infected focus. 40x magnification, scale bars 25 μm F) Individual channels of image shown in (E) demonstrating inconsistent co-localization of CD8 (aqua) and CD3 (purple). Scale bar 25 μm.

From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015) Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle.
PLoS ONE 10(11): e0143666.

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CD8 Antibody | CC63 gallery image 5

Published customer image:
RPE conjugated Mouse anti Bovine CD8 antibody, clone CC63 (MCA837PE) used to detect cytotoxic T-cells in bovine peripheral blood by flow cytometry together with APC conjugated Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653) used for the identification of helper T-cells in the same samples.
Image caption:
Insert image caption

From: Sun F, Cao Y, Cai C, Li S, Yu C, Yao J (2016)
Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine.
PLoS ONE 11(8): e0160659.

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Figure A. FITC conjugated mouse anti bovine CD2 (MCA833F) and RPE conjugated mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated mouse anti bovine CD2 (MCA833F) and RPE conjugated mouse anti bovine CD8 (MCA837PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

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CD8 Antibody | CC63 gallery image 7

Figure A. RPE conjugated mouse anti bovine CD8 (MCA837PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti bovine CD4 (MCA837PE) and FITC conjugated mouse anti bovine CD2 (MCA833F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

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CD8 Antibody | CC63 gallery image 8

Figure A. A647 conjugated mouse anti bovine CD26 (MCA1652A647) and FITC conjugated mouse IgG2a isotype control (MCA929F). Figure B. A647 conjugated mouse anti bovine CD26 (MCA1652A647) and FITC conjugated mouse anti bovine CD8 (MCA837F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

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CD8 Antibody | CC63 gallery image 9

Figure A. FITC conjugated mouse anti bovine CD8 (MCA837F) and A647 conjugated mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated mouse anti bovine CD8 (MCA837F) and A647 conjugated mouse anti bovine CD26 (MCA1652A647). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

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  • Product Type
    Monoclonal Antibody
  • Clone
    CC63
  • Isotype
    IgG2a
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA837GAC, F, IF, IP, Pdatasheet pdfdatasheet pdf0.1 mg
    MCA837GA
    MCA837FFdatasheet pdfdatasheet pdf100 Tests
    MCA837F
    MCA837PEFdatasheet pdfdatasheet pdf100 Tests
    MCA837PE
    MCA837A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA837A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine CD8 antibody, clone CC63 reacts with the bovine CD8 antigen expressed by a subset of T lymphocytes. The antibody precipitates molecules of 34kD and 38kD under reducing conditions. Clone CC63 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Gutierrez et al. 1999).
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Sheepyes
      Goatyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      This product should be stored undiluted.

      DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      GO:0016021
      integral to membrane
      Related reagents
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD8 Antibody Formats

    Formats Clone Applications Sizes available
    CD8 Antibody : RPE CC63 F 100 Tests
    CD8 Antibody : Alexa Fluor® 647 CC63 F 100 Tests/1ml
    CD8 Antibody : Purified CC63 C, F, IF, IP, P 0.1 mg
    CD8 Antibody : FITC CC63 F 100 Tests
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              1. MacHugh, N.D. & Sopp P (1991) Individual antigens of cattle. Bovine CD8 (BoCD8).
              Vet Immunol Immunopathol. 27 (1-3): 65-9.
              2. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
              Vet Immunol Immunopathol. 71 (3-4): 321-34.
              3. Twizere, J.C. et al. (2000) Discordance between bovine leukemia virus tax immortalization in vitro and oncogenicity in vivo.
              J Virol. 74 (21): 9895-902.
              4. Winkler, M.T. et al. (1999) Bovine herpesvirus 1 can infect CD4(+) T lymphocytes and induce programmed cell death during acute infection of cattle.
              J Virol. 73 (10): 8657-68.
              5. Winkler, M.T. et al. (2000) Persistence and reactivation of bovine herpesvirus 1 in the tonsils of latently infected calves.
              J Virol. 74 (11): 5337-46.
              6. Sidders, B. et al. (2008) Screening of highly expressed mycobacterial genes identifies Rv3615c as a useful differential diagnostic antigen for the Mycobacterium tuberculosis complex.
              Infect Immun. 76: 3932-9.
              7. Sanchez, J. et al. (2011) Microscopical and immunological features of tuberculoid granulomata and cavitary pulmonary tuberculosis in naturally infected goats.
              J Comp Pathol. 145 (2-3): 107-17.
              8. La Manna, M.P. et al. (2011) Expansion of intracellular IFN-γ positive lymphocytes during Mycoplasma agalactiae infection in sheep.
              Res Vet Sci. 91 (3): e64-7.
              9. Fulton, B.E. Jr. et al. (2006) Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node.
              J Virol. 80: 7873-84.
              10. Harris, J. et al. (2002) Expression of caveolin by bovine lymphocytes and antigen-presenting cells.
              Immunology. 105: 190-5.
              11. Liebana, E. et al. (2007) Distribution and activation of T-lymphocyte subsets in tuberculous bovine lymph-node granulomas.
              Vet Pathol. 44: 366-72.
              12. Lynch, E.M. et al. (2010) Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves.
              BMC Vet Res. 6: 39.
              13. Lacroux, C. et al. (2012) Prionemia and leukocyte-platelet-associated infectivity in sheep transmissible spongiform encephalopathy models.
              J Virol. 86 (4): 2056-66.
              14. Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.
              Vet Res. 41: 14.
              15. Toman, M. et al. (2003) Immunological characteristics of ca??le with Mycobacterium avium subsp. paratuberculosis infection
              Vet. Med. – Czech, 48, 2003: 147–54
              16. Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus0 cattle.
              Int J Parasitol. 40: 865-75.
              17. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
              Theriogenology. 82 (3): 403-10.
              18. Romero-Palomo F et al. (2015) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
              Transbound Emerg Dis. Aug 25. [Epub ahead of print]
              19. Stenfeldt, C. et al. (2015) Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle.
              PLoS One. 10 (11): e0143666.
              20. Leite FL et al. (2015) ZAP-70, CTLA-4 and proximal T cell receptor signaling in cows infected with Mycobacterium avium subsp. paratuberculosis.
              Vet Immunol Immunopathol. 167 (1-2): 15-21.

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