BrdU Labeling of HeLa Cells Followed by Immunostaining
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For use with immunocytochemistry tested Rat Anti-BrdU Antibody, Clone RF06
This method provides a general procedure for use with the majority of Bio-Rad reagents. In some cases, specific recommendations are provided on product datasheets, and these methods should always be used in conjunction with product and batch specific information provided with each vial. Note that a certain level of technical skill and immunological knowledge is required for the successful design and implementation of these techniques — these are guidelines only and may need to be adjusted for particular applications.
Please also note that this protocol has been developed for the BrdU labeling and immunostaining of HeLa cells. Therefore, it will need to be optimized for use in other cell types.
(Optional) PureBlu DAPI Nuclear Staining Dye (1351303) and antibody to stain cytoplasmic proteins, for example, Rabbit Anti-GAPDH Antibody (AHP1628)