Epstein-Barr Virus Nuclear Antigen Antibody | E1-2.5

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Epstein-Barr Virus Nuclear Antigen Antibody | E1-2.5 gallery image 1

Published customer image:
Mouse anti Epstein-Barr Virus Nuclear Antigen antibody, clone E1-2.5 used for the detection of EBNA-1 in cell extracts by western blotting.
Image caption:
(a) Epitope comparison amongst the variants and those observed in the 3 cell lines studied. Red letters denote amino acid mutation. (b) Quantitation of HLA-A0201/LMP1,/LMP2A and/EBNA1 on CCRF-SB, RPMI-6666 and C666-1A2 using TCR-like mAbs based on flow cytometry. Error bars are standard deviation from an average of three independent experiments. **p < 0.01. n.d. denotes not detectable. (c) LMP1, LMP2A and EBNA1 in each of the cell lines was determined using RT-PCR and Western blot. β-actin was used as control.

From: Sim AC, Too CT, Oo MZ, Lai J, Eio MY, Song Z, Srinivasan N, Tan DA, Pang SW, Gan SU, Lee KO, Loh TK, Chen J, Chan SH, MacAry PA. Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies.
Sci Rep. 2013 Nov 18;3:3232.

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  • Mouse anti Epstein-Barr Virus Nuclear Antigen
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  • Product Type
    Monoclonal Antibody
  • Clone
    E1-2.5
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2707Edatasheet pdfdatasheet pdf0.1 mg
    MCA2707
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Epstein-Barr Virus Nuclear Antigen antibody, clone E1-2.5 recognises the repetitive Gly-Ala region of EBV (Epstein-Barr Virus) nuclear antigen 1 (EBNA-1), a viral protein expressed consistently by EBV- associated malignancies.

      Epstein-Barr Virus, also known as HHV-4 or Human herpesvirus 4, is a member of the herpesvirus family and one of the most common infectious viruses known, being responsible for a wide range of illnesses from infectious mononucleosis to nasal-pharyngeal cancer. EBNA-1 is the only viral protein expressed during group 1 latency and mediates the replication and partitioning of the episome during host cell division, binding to a replication origin (oriP) within the viral genome. The Gly-Ala repeat recognized by Mouse anti Epstein-Barr Virus Nuclear Antigen antibody, clone E1-2.5, inhibits the host’s CD8-restricted cytotoxic T-cell response to infected cells, by impairing antigen processing and MHC class I-restricted antigen presentation.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      regulation of transcription
      protein binding
      transcription
      DNA binding
      host cell nucleus
      regulation of DNA replication
      evasion by virus of host immune response
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Epstein-Barr Virus Nuclear Antigen Antibody Formats

    Formats Clone Applications Sizes available
    Epstein-Barr Virus Nuclear Antigen Antibody : Purified E1-2.5 E 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

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              References

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