Western Blotting Buffers and Accessories

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Western Blot Detection of IP Samples - Pocket Guide

Western Blot Detection of IP Samples - New Pocket Guide

Our new pocket guide contains a set of steps to help you with your experimental design.

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Optimize your experiments

We understand how challenging Western blot experiments can be at times. Especially background staining from inadequate blocking or from unspecific secondary antibody binding may cause problems with the evaluation and interpretation of your results. In addition to Western blot loading controls that assist with the quantification of your Western blot data we offer a range of specialized reagents for improving your signal to noise ratio:

  • AbGuard® AP Stabilizer  - is an optimized diluent for the long term storage of alkaline phosphatase conjugated antibodies and other conjugated proteins. It provides superior stabilization of AP-conjugates at low and high dilutions, preventing the loss of phosphatase activity.
  • AbGuard® HRP Stabilizer - is an optimized diluent for the long term storage of peroxidase conjugated antibodies and other conjugated proteins. It provides superior stabilization of HRP-conjugates at low and high dilutions, preventing the loss of peroxidase activity.
  • Block ACE - high-performance Western blotting blocking reagent. Block ACE can also be used as a blocking reagent in immunoassay experiments and has been shown to result in better blocking results compared to BSA .
  • TidyBlot Western blot detection reagent:HRP - exclusively binds to native non-denatured antibodies and not to any IgGs present in your immunoprecipitate or lysate. Avoid obstruction by IgG heavy and light chains and produce background free Western blots. In addition TidyBlot detects a variety of monoclonal and polyclonal antibodies - no need to buy several HRP conjugated secondary antibodies.

Buffers and Accessories

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code

    AbGuard® is a registered trademark of MorphoSys UK Ltd