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Horseradish-peroxidase conjugated antibodies are preferentially used whenever an indirect method is used for detection on Western Blots. Bio-Rad supplies a range of species specific antibodies recognizing immunoglobulin or any of its isotypes. Monoclonal and polyclonal secondary antibodies are available, as a whole IgG or a F(ab’)2 fragment.
Performance is further enhanced by affinity purification for all STAR reagents and many anti-mouse, -rat, and -hamster secondary antibodies are multi-species adsorbed.