CD230 (Prpsc) Antibody | 2G11

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CD230 (Prpsc) Antibody | 2G11 gallery image 1

Published customer image:
Mouse anti Sheep CD230 (Prpsc) antibody, clone 2G11 used for the detection of prion protein in ovine brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemistry of brain in donor and recipient sheep. Trigeminal nucleus from donor (A) and recipient (B) animals; intraneuronal PrPSc is evident only in the recipient animal (B). White matter from donor (C) and recipient (D) animals; globular white matter labelling is evident only in the donor animal (C). Scale bar in (A) and (B) indicates 100µm; in (C) and (D) indicates 50 µm. Antibody 2G11

From: Simmons MM, Moore SJ, Lockey R, Chaplin MJ, Konold T, et al. (2015) Phenotype Shift from Atypical Scrapie to CH1641 following Experimental Transmission in Sheep. PLoS ONE 10(2): e0117063.

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CD230 (Prpsc) Antibody | 2G11 gallery image 2

Published customer image:
Mouse anti sheep CD230 (PrPSc) antibody, clone 2G11 used for the detection of prion protein in ovine brain by paraffin embedded tissue blot analysis.
Image caption:
PET blot analysis identifying PrPSc in the tubular structures of the cortex and medulla in naturally scrapie-sick Sarda sheep. (A) Brains of scrapie-sick (left) and healthy sheep (right) as a control for PET blot analysis procedure. Scale bar = 100 µm. (B) Low magnification of transverse paraffin sections from kidneys of a naturally scrapie-sick and a healthy sheep demonstrating PrPSc in the medullary and cortical regions of the kidney. Dotted lines indicate the locus of the kidney capsule or the edge of the tissue section. The small transparent box indicates the region shown at higher magnification and resolution in figure C. Scale bar = 1 cm. (C) PrPSc deposits and tubular structure fit together in the cortical part of the kidney in scrapie-sick sheep. Scale bar = 100 µm. (D) PrPSc is also found to a high degree in the collecting ducts of the medulla (arrowhead) as well as in the papillae (arrow) of kidneys derived from naturally scrapie infected sheep. Scale bar = 30 µm.

From: Ligios C, Cancedda GM, Margalith I, Santucciu C, Madau L, et al. (2007) Intraepithelial and Interstitial Deposition of Pathological Prion Protein in Kidneys of Scrapie-Affected Sheep. PLoS ONE 2(9): e859.

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CD230 (Prpsc) Antibody | 2G11 gallery image 3

Published customer image:
Mouse anti sheep CD230 (PrPSc) antibody, clone 2G11 used for the detection of prion protein in ovine brain by immunohistochemistry and immunofluorescence on formalin fixed, paraffin embedded tisues sections.
Image caption:
PET blot analysis identifying PrPSc in the tubular structures of the cortex and medulla in naturally scrapie-sick Sarda sheep. (A) Brains of scrapie-sick (left) and healthy sheep (right) as a control for PET blot analysis procedure. Scale bar = 100 µm. (B) Low magnification of transverse paraffin sections from kidneys of a naturally scrapie-sick and a healthy sheep demonstrating PrPSc in the medullary and cortical regions of the kidney. Dotted lines indicate the locus of the kidney capsule or the edge of the tissue section. The small transparent box indicates the region shown at higher magnification and resolution in figure C. Scale bar = 1 cm. (C) PrPSc deposits and tubular structure fit together in the cortical part of the kidney in scrapie-sick sheep. Scale bar = 100 µm. (D) PrPSc is also found to a high degree in the collecting ducts of the medulla (arrowhead) as well as in the papillae (arrow) of kidneys derived from naturally scrapie infected sheep. Scale bar = 30 µm.

From: Ligios C, Cancedda GM, Margalith I, Santucciu C, Madau L, et al. (2007) Intraepithelial and Interstitial Deposition of Pathological Prion Protein in Kidneys of Scrapie-Affected Sheep. PLoS ONE 2(9): e859.

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CD230 (Prpsc) Antibody | 2G11 gallery image 4

Published investigator image:
Mouse anti Sheep CD230 (PrPSc) antibody, clone 2G11 used for the detection of prion protein in scrapie infected sheep by immunohistohemistry.
Image caption:
Photomicrographs of retinal cross-sections of unaffected (a, d) and naturally scrapie-affected (b, c, e) sheep. All layers of the control retina show normal organization and cytoarchitecture (a). Ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), photoreceptor segments (PS) and choroid (CHOR). Bar: 100 µm. In the scrapie-affected retina, not only was there a disorganization and thickening of the PS layer, but also an overall decrease of cellular density within the INL and ONL (b). Arrowhead indicates displaced photoreceptor nuclei in the PS layer. Bar: 100 µm. Immnohistochemical detection of abnormal PrP deposits (peroxydase-DAB, brown deposits-2G11 monoclonal mouse antibody) in a scrapie retina (c). Asterisks indicate the amorphous material accumulated in the photoreceptor outer segment layer. Bar: 100 µm. Müller cells labelling (GFAP rabbit polyclonal antibody peroxydase-DAB, brown coloration) in the retina from a control (d) and a naturally-scrapie affected sheep (e). Bars: 300 µm.

From: Regnier A, Andreoletti O, Albaric O, Gruson DC, Schelcher F, Toutain PL. Clinical, electroretinographic and histomorphometric evaluation of the retina in sheep with natural scrapie. BMC Vet Res. 2011 Jun 6;7:25.

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  • Mouse anti Sheep CD230 (Prpsc)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2G11
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2460E, P *datasheet pdfdatasheet pdf0.25 mg
    MCA2460
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Sheep CD230 (Prpsc) antibody, clone 2G11 recognizes the prion disease form of prion protein known as CD230. Transmissible spongiform encephalopathies (TSEs) or prion diseases are fatal infectious neurodegenerative diseases of humans and animals. These diseases are biologically unique, as they are believed by some to be transmitted by an infectious agent comprised only of protein, with no nucleic acid component. Clinically, these diseases present with motor disturbances and behavioural changes. The major pathological changes seen are neuronal loss, vacuolation (spongiform change), proliferation and branching of glial cells, astrocytic proliferation and accumulation of the prion protein PrPSc, which can form amyloid plaques.

      CD230. also known as the prion protein (PrP) exists in two alternate forms; a normal cellular form (PrPc) and a disease-associated form (PrPSc).The normal and pathological forms of the prion protein have identical amino acid sequences and differ only in their folded tertiary structure and biochemical properties.

      MCA2460 is specific for PrPSc. This clone does not appear to immunostain the normall cellular PrP expressed in heathly sheep tissue sections resulting in specific labelling of ovine PrPSc. This antibody was raised against a synthetic peptide (146-R154R171-182 ) of the ovine PrP peptide and specificially recognises the R151-R159 sequence.
    • Intended Use
    • Target Species
      Sheep
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Synthetic peptide 146-R154R171-182 of ovine PrP.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      protein homooligomerization
      anchored to membrane
      Golgi apparatus
      microtubule binding
      plasma membrane
      copper ion binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/501/1000
      Immunohistology - Paraffin(1)
      (1)
      Treatment of tissue sections in 98% formic acid, for 30 minutes, is recommended prior to pre-treatment with trypsin at 37oC for 5 minutes followed by heat mediated retrieval with 10mM citrate buffer pH6.0 See Andreoletti 2000.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      Tested on peptide.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD230 (Prpsc) Antibody Formats

    Formats Clone Applications Sizes available
    CD230 (Prpsc) Antibody : Purified 2G11 E, P * 0.25 mg
    • Copyright © 2016 Bio-Rad

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