CD45RA Antibody | OX-33

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CD45RA Antibody | OX-33 gallery image 1

Staining of rat spleen cells with Mouse anti Rat CD45RA: FITC (MCA340FB)

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CD45RA Antibody | OX-33 gallery image 2

Staining of rat spleen cells with Mouse anti Rat CD45RA:Alexa Fluor® 488 (MCA340A488)

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  • CD45RA Antibody | OX-33 thumbnail image 1
  • CD45RA Antibody | OX-33 thumbnail image 2
  • Mouse anti Rat CD45RA:RPE-Alexa Fluor® 647
  • Mouse anti Rat CD45RA:FITC
  • Mouse anti Rat CD45RA:RPE
  • Mouse anti Rat CD45RA (B Cells Only)
  • Mouse anti Rat CD45RA (B Cells Only)
  • Mouse anti Rat CD45RA:Alexa Fluor® 488
  • Mouse anti Rat CD45RA (B Cells Only)
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  • Product Type
    Monoclonal Antibody
  • Clone
    OX-33
  • Isotype
    IgG1
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA340FTFdatasheet pdfdatasheet pdf0.1 mg
    MCA340FT
    MCA340GAC, F, P *datasheet pdfdatasheet pdf0.1 mg
    MCA340GA
    MCA340RC, F, P *datasheet pdfdatasheet pdf0.25 mg
    MCA340R
    MCA340GC, F, P *datasheet pdfdatasheet pdf1 mg
    MCA340G
    MCA340PEFdatasheet pdfdatasheet pdf100 Tests
    MCA340PE
    MCA340P647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA340P647
    MCA340A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA340A488
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD45RA antibody, clone OX-33 is directed against a high molecular weight band of the leucocyte common antigen. MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labelling in bone marrow and none on thymocytes (Barclay et al. 1987).
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE)-Alexa Fluor® 647 - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
    • Immunogen
      Purified Rat spleen L-CA
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
    • Storage
      Store at +4oC.
      DO NOT FREEZE.
      This product should be stored undiluted.
      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of reconstitution
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      membrane raft
      B cell receptor signaling pathway
      focal adhesion
      integral to plasma membrane
      negative regulation of protein kinase activity
      transmembrane receptor protein tyrosine phosphatase activity
      release of sequestered calcium ion into cytosol
      T cell receptor signaling pathway
      positive regulation of antigen receptor-mediated signaling pathway
      protein kinase regulator activity
      negative regulation of T cell mediated cytotoxicity
      B cell proliferation
      negative regulation of cytokine-mediated signaling pathway
      protein dephosphorylation
      defense response to virus
      internal side of plasma membrane
      response to gamma radiation
      protein kinase binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen1/1001/500
      Immunohistology - Paraffin(1)
      (1)
      PLP fixation is recommended for optimal results, see Whiteland et al. for details
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen1/1001/500
      Immunohistology - Paraffin(1)
      (1)
      PLP fixation is recommended for optimal results, see Whiteland et al. for details
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen1/1001/500
      Immunohistology - Paraffin(1)
      (1)
      PLP fixation is recommended for optimal results, see Whiteland et al. for details

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD45RA Antibody Formats

    Formats Clone Applications Sizes available
    CD45RA Antibody : Purified OX-33 C, F, P * 1 mg | 0.1 mg | 0.25 mg
    CD45RA Antibody : FITC OX-33 F 0.1 mg
    CD45RA Antibody : RPE-Alexa Fluor® 647 OX-33 F 100 Tests/1ml
    CD45RA Antibody : Alexa Fluor® 488 OX-33 F 100 Tests/1ml
    CD45RA Antibody : RPE OX-33 F 100 Tests
    • Copyright © 2016 Bio-Rad

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
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              References

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