CD35 antibody | E11
Mouse anti Human CD35:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- E11
- Isotype
- IgG1
- Specificity
- CD35
Mouse anti Human CD35 antibody, clone E11 recognizes complement receptor type 1, also known as CD35 or the C3b/C4b receptor. CD35 a single chain, type I single pass transmembrane glycoprotein which exists in four allotypic forms (A,B,C,D) of ~190 kDa, ~220 kDa, ~160 kDa and ~250 kDa respectively (Khera et al. 2009). CD35 is expressed by granulocytes, monocytes, B cells and some T cells. Functionally, CD35 acts as a receptor for the C3b component of complement. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey Baboon - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Human acute monocytic leukaemia cells.
- Approx. Protein Concentrations
- IgG concentration 1mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/50 | 1/100 | |
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Flow Cytometry
- Use 10ul of the diluted antibody to label 106 cells or 100ul whole blood.
- Histology Positive Control Tissue
- Tonsil
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control |
Source Reference
-
Hogg, N. et al. (1984) Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1).
Eur J Immunol. 14 (3): 236-43.
References for CD35 antibody
-
Birmingham, D.J. et al. (1996) The baboon erythrocyte complement receptor is a glycophosphatidylinositol-linked protein encoded by a homologue of the human CR1-like genetic element.
J Immunol. 157 (6): 2586-92. -
Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys.
Cytometry. 29 (4): 351-62. -
Weissgerber P et al. (2003) Investigation of mechanisms involved in phagocytosis of Legionella pneumophila by human cells.
FEMS Microbiol Lett. 219 (2): 173-9. -
Schmidt, C.Q. et al. (2016) Selectivity of C3-opsonin targeted complement inhibitors: A distinct advantage in the protection of erythrocytes from paroxysmal nocturnal hemoglobinuria patients.
Immunobiology. 221 (4): 503-11. -
Noda, G.S. et al. (2020) Specificities and isotypes of erythrocytes autoantibodies in patients with warm autoimmune hemolytic anemia
Rev Cubana Hematol Inmunol Hemoter 36(4): e1283. -
Klaas, M. et al. (2023) Sialoadhesin (CD169/Siglec-1) is an extended molecule that escapes inhibitory cis-interactions and synergizes with other macrophage receptors to promote phagocytosis.
Glycoconj J. 40 (2): 213-23. -
Wagstaffe, H.R. et al. (2023) The Development of Immunological Assays to Evaluate the Level and Function of Antibodies Induced by Klebsiella pneumoniae O-Antigen Vaccines.
mSphere. : e0068022.
- Synonyms
- CR1
- RRID
- AB_323143
- UniProt
- P17927
- Entrez Gene
- CR1
- GO Terms
- GO:0004875 complement receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
MCA554XZ
153806 1805If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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