IgG1 antibody
Phycoerythrin conjugated Goat anti Mouse IgG1 antibody (STAR132PE) used for the detection of bound antibody on lymphoblastoid cell lines by flow cytometry.
Image caption:
Estimation of ΔBNLF2a and wild-type LCLs expressing lytic antigens; recognition by immediate early antigen-specific CD8+ T cells. The proportion of LCLs spontaneously reactivating into lytic cycle was assessed by intracellular BZLF1 staining and analysis by flow cytometry, with representative examples shown for LCLs derived from two different donors: (A) donor 1 and (B) donor 2. Immediate early lytic cycle CD8+ T cell recognition of wild-type (wt), ΔBNLF2a (Δ2a) and ΔBZLF1 (ΔBZ) LCLs using HLA-B*0801-restricted RAK (BZLF1) clones against appropriately HLA matched donor 1 and 2 LCLs (C and D respectively) was measured by IFNγ ELISA. Results using wild-type or ΔBNLF2a cells diluted with ΔBZLF1 cells as appropriate are shown, where arrows indicate equivalent numbers of lytic antigen expressing cells. Experiments were also conducted using HLA-C*0202-restricted IACP (BRLF1) clones against donor 3 LCLs (E), and HLA-B*4501-restricted AEN (BRLF1) clones against donor 4 LCLs (F). For donor 4, both the wild-type and ΔBNLF2a LCLs were diluted with ΔBZLF1 LCL (wild-type-LCL titration data not shown). Data are represented as mean+/−SEM.
From: Croft NP, Shannon-Lowe C, Bell AI, Horst D, Kremmer E, et al. (2009) Stage-Specific Inhibition of MHC Class I Presentation by the Epstein-Barr Virus BNLF2a Protein during Virus Lytic Cycle. PLoS Pathog 5(6): e1000490.
Horseradish peroxidase conjugated Goat anti Mouse IgG1 antibody (STAR132P) used for the evaluation of rotavirus specific IgG1 antibodies in mouse serum be ELISA.
Image caption:
Rotavirus-specific IgG-subclass antibodies in serum. Geometric mean titers (GMT) in arbitrary units (AU) ± SEM of the rotavirus-specific IgG-subclass antibodies IgG1, IgG2a, IgG2b and IgG3 in serum individually collected at day 28, depicted per antibody.
From: Knipping et al.
A gastrointestinal rotavirus infection mouse model for immune modulation studies
Virology Journal 2011 8:109.
FITC conjugated Goat anti Mouse IgG1 antibody (STAR132F) used for the detection of bound Mouse anti BrdU antibody by immunofluorescence.
Image caption:
Sequential labelling using EdU and BrdU. Microscopy pictures of cells double-labelled with EdU and BrdU. Cells synchronized in G1 phase were released and pulse-labelled in two consecutive S-phases, first with EdU, then with BrdU, as described in the text. Samples were harvested after the next mitosis, fixed and processed as described and imaged using fluorescence microscopy.
From: Anda S, Boye E, Grallert B (2014) Cell-Cycle Analyses Using Thymidine Analogues in Fission Yeast. PLoS ONE 9(2): e88629.
FITC conjugated Goat anti Mouse IgG1 antibody (STAR132F) used for the detection of bound Mouse anti BrdU antibody by immunofluorescence.
Image caption:
Microscopy pictures of cells labelled with EdU or BrdU. Cells were labelled with either analogue and detection for both analogues was performed to check cross-reactivity.
From: Anda S, Boye E, Grallert B (2014) Cell-Cycle Analyses Using Thymidine Analogues in Fission Yeast. PLoS ONE 9(2): e88629.
Goat anti Mouse IgG1:Alk. Phos.
Goat anti Mouse IgG1:FITC
Goat anti Mouse IgG1:HRP
Goat anti Mouse IgG1
Goat anti Mouse IgG1:RPE
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| STAR132A | C E P WB | 1 ml |
|
|
| STAR132F | C F IF | 0.5 mg |
|
|
| STAR132P | C E P WB | 1 ml |
|
|
| STAR132 | E | 0.5 mg |
|
|
| STAR132PE | F | 0.1 mg |
|
Goat anti Mouse IgG1 antibody recognizes Mouse IgG1. This antibody has been cross absorbed against mouse IgM, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Goat anti Mouse IgG1 antibody shows minimal cross-reactivity with human immunoglobulins.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Antiserum Preparation
- Antisera to mouse IgG1 were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- 50mM TRIS
1mM MgCl2 - Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Borate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1%Sodium Azide (NaN3)
50% Glycerol - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.01% Thiomersal
50% Glycerol - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide Sucrose - Immunogen
- Mouse IgG1 paraproteins.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 6 months from date of despatch
More Information
- UniProt
- P01869
- P01868
- Entrez Gene
- Ighg1
- Ighg1
- GO Terms
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0003823 antigen binding
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0006958 complement activation, classical pathway
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0050766 positive regulation of phagocytosis
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- Regulatory
- For research purposes only
Applications of IgG1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2000 | 1/4000 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting | |||
| Flow Cytometry | 1/1000 | ||
| Immunofluorescence | Neat | 1/10 | |
| Immunohistology - Frozen | |||
| ELISA | 1/4000 | 1/8000 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting | |||
| ELISA | |||
| Flow Cytometry | 1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml |
Product Specific References
References for IgG1 antibody
-
Croft, N.P. et al. (2009) Stage-specific inhibition of MHC class I presentation by the Epstein-Barr virus BNLF2a protein during virus lytic cycle.
PLoS Pathog. 5(6): e1000490. -
Zuo, J. et al. (2011) The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting MHC class I molecules trafficking on both the exocytic and endocytic pathways.
J Virol. 85: 1604-14. -
Knipping, K. et al. (2011) A gastrointestinal rotavirus infection mouse model for immune modulation studies.
Virol J. 8: 109. -
Young, D. et al. (2012) Soy-derived di- and tripeptides alleviate colon and ileum inflammation in pigs with dextran sodium sulfate-induced colitis.
J Nutr. 142 (2): 363-8. -
Bagai, U. and Pawar, A. (2013) A blood stage fraction of Plasmodium berghei induces protective and long lasting immune response in BALB/c mice.
Parasitol Int. 62: 329-36. -
Anda, S. et al. (2014) Cell-cycle analyses using thymidine analogues in fission yeast.
PLoS One. 9 (2): e88629. -
Kamat, M.M. et al. (2016) Changes in Myeloid Lineage Cells in the Uterus and Peripheral Blood of Dairy Heifers During Early Pregnancy.
Biol Reprod. Aug 10. pii: biolreprod.116.141069. [Epub ahead of print] -
Ramanathan, R. et al. (2015) Transplantation of human stem cell-derived hepatocytes in an animal model of acute liver failure.
Surgery. 158 (2): 349-59. -
Hwang, S.R. et al. (2015) Altered expression levels of neurodevelopmental proteins in fetal brains of BTBR T+tf/J mice with autism-like behavioral characteristics.
J Toxicol Environ Health A. 78 (8): 516-23. -
Zhao, Z. et al. (2015) Multiple B-cell epitope vaccine induces a Staphylococcus enterotoxin B-specific IgG1 protective response against MRSA infection.
Sci Rep. 5: 12371. -
Gutiérrez-Miranda, B. et al. (2020) Oleacein Attenuates the Pathogenesis of Experimental Autoimmune Encephalomyelitis through Both Antioxidant and Anti-Inflammatory Effects.
Antioxidants (Basel). 9 (11)Nov 21 [Epub ahead of print]. -
Gutierrez, B. et al. (2020) Oleanolic acid ameliorates intestinal alterations associated with EAE
Journal of Neuroinflammation. 17 (1) [Epub ahead of print]. -
Apóstolo, N. et al. (2020) Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer.
Nat Commun. 11 (1): 5171. -
Zhuang, X. et al. (2020) CAR T cells targeting tumor endothelial marker CLEC14A inhibit tumor growth.
JCI Insight. 5 (19) Oct 02 [Epub ahead of print].
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