IgG antibody

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Anti Goat IgG (Fc) Antibody thumbnail image 1

Anti Goat IgG (Fc) Antibody gallery image 1 — **Published customer image:**
Rabbit anti Goat IgG (Fc) antibody (**STAR122P**) used as a secondary detection antibody to recognize goat primaries against CINC-1 and neutrophil elastase in rat lung sections by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
**Image caption:**
Decrease in airway accumulation of neutrophils and CINC-1 following treatment with S-maltoheptaose.
(A) Lung tissue sections were stained for NE (a, c, e) or CINC-1 (b, d, f) and then counter-stained with haematoxylin. NE-positive cells lining the basal side of the bronchial epithelium were rarely observed in cases treated with S-maltoheptaose (a vs c, arrows). CINC-1 immuno-positivity enriched along the luminal and basal sides of the bronchial epithelium were barely observable in cases treated with S-maltoheptaose (b vs d, arrowheads). Neither immuno-positivity for NE nor CINC-1 was observable in the sham air controls (e & f). *bronchial lumen. Scale bar = 100μm.

From: Lam DC, Chan SC, Mak JC, Freeman C, Ip MS, Shum DK.
S-maltoheptaose targets syndecan-bound effectors to reduce smoking-related neutrophilic inflammation.
Sci Rep. 2015 Aug 10;5:12945.

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Rabbit anti Goat IgG (Fc):FITC

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: IgG
Region: (Fc)

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References (2)
Applications
Storage Information
Batch/Lot Specific Datasheets

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Rabbit anti goat IgG recognizes an epitope localised to the Fc region of goat immunoglobulin G as assessed by immunoelectrophoresis and ELISA.

Rabbit anti goat IgG may cross react with IgG from other species.

Target Species: Goat
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation: Antisera to goat IgG were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

FITC 490 525
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525

Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		1/200	1/2,000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References for IgG antibody

Corrales, L. et al. (2012) Anaphylatoxin C5a creates a favorable microenvironment for lung cancer progression.
J Immunol. 189 (9): 4674-83.
Lam, D.C. et al. (2015) S-maltoheptaose targets syndecan-bound effectors to reduce smoking-related neutrophilic inflammation.
Sci Rep. 5: 12945.