IgG antibody
Rabbit anti Goat IgG (Fc) antibody (STAR122P) used as a secondary detection antibody to recognize goat primaries against CINC-1 and neutrophil elastase in rat lung sections by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Decrease in airway accumulation of neutrophils and CINC-1 following treatment with S-maltoheptaose.
(A) Lung tissue sections were stained for NE (a, c, e) or CINC-1 (b, d, f) and then counter-stained with haematoxylin. NE-positive cells lining the basal side of the bronchial epithelium were rarely observed in cases treated with S-maltoheptaose (a vs c, arrows). CINC-1 immuno-positivity enriched along the luminal and basal sides of the bronchial epithelium were barely observable in cases treated with S-maltoheptaose (b vs d, arrowheads). Neither immuno-positivity for NE nor CINC-1 was observable in the sham air controls (e & f). *bronchial lumen. Scale bar = 100μm.
From: Lam DC, Chan SC, Mak JC, Freeman C, Ip MS, Shum DK.
S-maltoheptaose targets syndecan-bound effectors to reduce smoking-related neutrophilic inflammation.
Sci Rep. 2015 Aug 10;5:12945.
Filter by Application:
P ResetRabbit anti Goat IgG (Fc):HRP
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
- Region
- (Fc)
Antibody Quality and Performance Guarantee
| Rabbit anti goat IgG recognizes an epitope localised to the Fc region of goat immunoglobulin G as assessed by immunoelectrophoresis and ELISA. Rabbit anti goat IgG may cross react with IgG from other species. |
- Target Species
- Goat
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Antiserum Preparation
- Antisera to goat IgG were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.05% ProClin 300
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/ 10,000 | 1/ 100,000 |
| Immunohistology - Frozen | |
1/200 | 1/5000 |
| Western Blotting | |
1/5000 | 1/50000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| TMB Signal+ | BUF054A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TMB Signal+ | ||||||
| TMB Core | BUF056A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TMB Core | ||||||
| TMB Core+ | BUF062A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TMB Core+ | ||||||
References for IgG antibody
-
Corrales, L. et al. (2012) Anaphylatoxin C5a creates a favorable microenvironment for lung cancer progression.
J Immunol. 189 (9): 4674-83. -
Lam, D.C. et al. (2015) S-maltoheptaose targets syndecan-bound effectors to reduce smoking-related neutrophilic inflammation.
Sci Rep. 5: 12945.
- RRID
- AB_567020
STAR122P
148822
158452
165241
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