CD45 Antibody | K252.1E4

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CD45 Antibody | K252.1E4 gallery image 1

Staining of pig peripheral blood lymphocytes with Mouse anti Pig CD45:FITC (MCA1222F)

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Staining of pig peripheral blood lymphocytes with Mouse anti Pig CD45:Alexa Fluor®647(MCA1222A647)

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Published customer image:
Mouse anti Pig CD45 antibody, clone K252.1E4 used for the identification of contaminating leukocytes in bone marrow derived mesenchymal sem cell cultures by flow cytomery.
Image caption:
Characterization of pig BM-MSCs. (A) Single-cell suspension plated at 4000 cells/cm2 formed abundant colony forming units (CFUs, purple dots) after 9 days of culturing. The average number of CFUs (>50 cells/CFU) was 158.8±7.0. (B–D) Upon proper induction, osteogenic (B), chondrogenic (C) and adipogenic (D) differentiations of pig BM-MSC were confirmed by their characteristic stains, respectively (arrows). Calibration bars: 50 μm. (E–F) Flow cytometric analysis of obtained pig BM-MSCs in the first three passages showed positive expression of MSC surface markers (CD44 and CD105; E) and negative expression of hematopoietic surface markers (CD45 and CD11b; F).

From: Sun Z, Tee BC, Kennedy KS, Kennedy PM, Kim D-G, Mallery SR, et al. (2013) Scaffold-Based Delivery of Autologous Mesenchymal Stem Cells for Mandibular Distraction Osteogenesis: Preliminary Studies in a Porcine Model.
PLoS ONE 8(9): e74672.

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Figure A. FITC conjugated mouse anti porcine CD45 (MCA1222F) and purified mouse IgG2a isotype control (MCA929) detected with goat anti mouse IgG2a PE (STAR133PE). Figure B. FITC conjugated mouse anti porcine CD45 (MCA1222F) and purified mouse anti porcine CD6 (MCA1221GA) detected with goat anti mouse IgG2a (STAR133PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Figure A. Purified mouse anti porcine CD6 (MCA1221GA) detected with goat anti mouse IgG2a PE (STAR133PE) and mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified mouse anti porcine CD6 (MCA1221GA) detected with goat anti mouse IgG2a PE (STAR133PE) and mouse anti porcine CD45 FITC (MCA1222F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Figure A. FITC conjugated mouse anti porcine CD14 (MCA1218F) and A647 conjugated mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated mouse anti porcine CD14 (MCA1218F) and A647 conjugated mouse anti porcine CD45 (MCA1222A647). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Figure A. A647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. A647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse anti porcine CD14 (MCA1218F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Figure A. FITC conjugated mouse anti porcine CD11R3 (MCA2309F) and APC conjugated mouse IgG1 isotype control (MCA928APC). Figure B. FITC conjugated mouse anti porcine CD11R3 (MCA2309F) and APC conjugated mouse anti porcine CD31 (MCA1222APC). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Figure A. FITC conjugated mouse anti porcine CD45 (MCA1222F) and PE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti porcine CD45 (MCA1222F) and PE conjugated mouse anti porcine CD16 (MCA1971PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Figure A. PE conjugated mouse anti porcine CD16 (MCA1971PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. PE conjugated mouse anti porcine CD16 (MCA1971PE) and FITC conjugated mouse anti porcine CD45 (MCA1222F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Figure A. A647 conjugated mouse anti porcine CD45 (MCA1222A647) and A488 conjugated mouse IgG1 isotype control (MCA928A488). Figure B. A647 conjugated mouse anti porcine CD45 (MCA1222PE) and A488 conjugated mouse anti porcine CD117 (MCA2598A488). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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  • Mouse anti Pig CD45:FITC
  • Mouse anti Pig CD45:Alexa Fluor® 647
  • Mouse anti Pig CD45
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    K252.1E4
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1222GAC, F, IFdatasheet pdfdatasheet pdf0.1 mg
    MCA1222GA
    MCA1222FFdatasheet pdfdatasheet pdf100 Tests
    MCA1222F
    MCA1222A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1222A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Pig CD45, clone K252.1E4 recognizes an epitope common to all porcine CD45 isoforms (Schnitzlein et al. 1998). CD45 is also known as leukocyte common antigen (LCA).

      Mouse anti Pig CD45, clone K252.1E4 immunoprecipitates three polypeptides of 226, 210 and 190 kDa from preparations of porcine peripheral blood mononuclear cells and shows a broad reactivity pattern with both lymphoid and myeloid cells (Zuckermann et al. 1994).
    • Intended Use
    • Target Species
      Pig
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alexa Fluor 647 - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A.
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Porcine Peripheral Blood lymphocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 0.05mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
      Immunofluorescence
      Immunohistology - Frozen

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD45 Antibody Formats

    Formats Clone Applications Sizes available
    CD45 Antibody : Purified K252.1E4 C, F, IF 0.1 mg
    CD45 Antibody : FITC K252.1E4 F 100 Tests
    CD45 Antibody : Alexa Fluor® 647 K252.1E4 F 100 Tests/1ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647100 Tests/1mlF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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