CD169 Antibody | 3D6.112

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CD169 Antibody | 3D6.112 gallery image 1

Immunohistochemical staining of frozen mouse spleen with Rat anti Mouse CD169 antibody, clone 3D6.112. (MCA884)

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CD169 Antibody | 3D6.112 gallery image 2

Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884) followed by Goat anti Rat IgG antibody (STAR72) as a detection reagent. Low power

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CD169 Antibody | 3D6.112 gallery image 3

Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884) followed by Goat anti Rat IgG antibody (STAR72) as a detection reagent. Medium power

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CD169 Antibody | 3D6.112 gallery image 4

Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884) followed by Goat anti Rat IgG antibody (STAR72) as a detection reagent. High power

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CD169 Antibody | 3D6.112 gallery image 5

Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 (MCA884), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 (MCA1108), red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

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CD169 Antibody | 3D6.112 gallery image 6

Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 (MCA884), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 (MCA1108), red in B. C is the merged image with nuclei counterstained blue using DAPI. High power

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CD169 Antibody | 3D6.112 gallery image 7

Published customer image:
Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin in mouse spleen sections by immunofluorescence.
Image caption:
Multicolor antibody array for mouse spleen using FDMM. Tissue sections from a wild type spleen (WT), a TNFα-receptor 1 knockout spleen (TNFaR1 KO), and a spleen from a CD11b-DTR mouse (CD11b-DTR) were multi-immunolabeled with antibodies against marginal zone macrophages (anti-CD169, cyan), dendritic cells (anti-CD11c, blue), B cells (anti-B220, red), CD4+ T cells (anti-CD4, green), and CD8+ T cells (anti-CD8, magenta). Nuclei were stained with DAPI (not shown). Arrow in TNFα-receptor 1 knockout spleen indicates weak marginal zone structure (cyan). Arrowhead in CD11b-DTR spleen indicates CD4+ T cells (green) within the B cell follicles. All six channels are presented individually in S6 Fig. Scale bar, 100 μm.

From: Kijani S, Yrlid U, Heyden M, Levin M, Borén J, et al. (2015) Filter-Dense Multicolor Microscopy. PLoS ONE 10(3): e0119499.

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CD169 Antibody | 3D6.112 gallery image 8

Published customer image:
Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.
Image caption:
Splenic marginal zone macrophages and marginal metallophilic macrophages control L. donovani by an IFN-γ independent mechanism. (A) L. donovani amastigotes are found predominantly in MMM and MZM after intravenous injection (purple, SIGNR1; green CD169; white, L. donovani; blue DAPI). (B) C57BL/6 mice were treated with 0.5mg XMG 1.6 anti-IFN-γ IgG (black bars) or control IgG (open bars) and 2h later infected with L. donovani. At 5h and 24h p.i., the number of parasites per MZ area was determined. (C) 14M1.4 cells (black bars) and RAW 264.7 cells (open bars) were infected with L. donovani (MOI 10:1) and at indicated times p.i., parasite numbers per 100 macrophages were determined by fluorescence microscopy. *, p<0.05, **, p<0.01 between bars indicated as indicated. Scale bar; 200μm.

From: Phillips R, Svensson M, Aziz N, Maroof A, Brown N, et al. (2010) Innate Killing of Leishmania donovani by Macrophages of the Splenic Marginal Zone Requires IRF-7. PLoS Pathog 6(3): e1000813.

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CD169 Antibody | 3D6.112 gallery image 9

Published customer image:
Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.
Image caption:
IRF-7 is induced in MZM and MM in situ. (A–D) Induction of IRF-7 in MMM and MZM. Naïve (A,B) and C57BL/6 mice infected for 24h (C, D) were stained for CD169 (A, C; green) or SIGNR1 (B, D; green), IRF-7 (red) and L. donovani (white). (E) The percentage of IRF-7+ parasite-containing phagosomes was quantified by counting 100 amastigotes/section (n = 3) and calculating the proportion of parasites with IRF-7 accumulation. *, p<0.05 (F) Snap shot from Video S4, showing infected MMM with phagosomal localisation of IRF-7 (CD169+ green, IRF-7, red; LV9, white; DAPI, blue). Scale bars; 50μm.

From: Phillips R, Svensson M, Aziz N, Maroof A, Brown N, et al. (2010) Innate Killing of Leishmania donovani by Macrophages of the Splenic Marginal Zone Requires IRF-7. PLoS Pathog 6(3): e1000813.

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CD169 Antibody | 3D6.112 gallery image 10

Published customer image:
Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.
Image caption:
Irf-7-/- MZM and MMM are defective in anti-leishmanial activity. (A–F) Parasite localisation in wild type and Irf-7-/- mice. Spleens from naïve C57BL/6 (A) and Irf-7-/- (B) mice and C57BL/6 (C, E) and Irf-7-/- (D, F) mice infected with L. donovani for 5h or 24h, were stained for CD169 (green), SIGNR1 (red), Leishmania (white) and counterstained with DAPI (blue). Higher magnification inserts show localization of amastigotes (arrowed). (G) Parasite load in the MZ of C57BL/6 mice (open bars) and B6.Irf-7-/- mice (black bars) was determined by counting the number of parasites per MZ area *, p<0.05, **, p<0.01. (H) The number of amastigotes in CD169+ MMM and SIGNR1+ MZM of B6 (open bars) and Irf-7-/- (black bars) mice was determined at 5h and 24h (n = 64 MZ profiles, from 4 mice per time point per strain). Data are presented as fold change at 24h. ***, p<0.001. Scale bars; 100μm.

From: Phillips R, Svensson M, Aziz N, Maroof A, Brown N, et al. (2010) Innate Killing of Leishmania donovani by Macrophages of the Splenic Marginal Zone Requires IRF-7. PLoS Pathog 6(3): e1000813.

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CD169 Antibody | 3D6.112 gallery image 11

Published customer image:
Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.
Image caption:
Specificity of IRF-7 polyclonal antibody. (A, B) 14M1.4 cells (A) and knockdown line KD#1 (B) were infected with L. donovani and stained at 24h for IRF-7. (C,D) L. donovani infected B6 mice (C) and B6.Irf7-/- mice (D) were infected for 5h and then stained for IRF-7. No phagosomal staining of IRF-7 was observed in KD#1 or in B6.Irf7-/- mice, confirming that this antibody does not cross react with Leishmania components.

From: Phillips R, Svensson M, Aziz N, Maroof A, Brown N, et al. (2010) Innate Killing of Leishmania donovani by Macrophages of the Splenic Marginal Zone Requires IRF-7. PLoS Pathog 6(3): e1000813.

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CD169 Antibody | 3D6.112 gallery image 12

Figure A. RPE conjugated rat anti mouse CD45R (MCA1258PE) and FITC conjugated Rat IgG2a isotype control (MCA1212F). Figure B. RPE conjugated rat anti mouse CD45R (MCA1258PE) and FITC conjugated rat anti mouse CD169 (MCA884F). All experiments performed on murine bone marrow in the presence of murine SeroBlock (BUF041A).

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  • Rat anti Mouse CD169
  • Rat anti Mouse CD169:FITC
  • Rat anti Mouse CD169:Low Endotoxin
  • Rat anti Mouse CD169
  • Rat anti Mouse CD169:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti Murine CD169 antibody identifies a receptor expressed by a restricted set of macrophages, a receptor for alpha 2,3 linked sialic acid residues on spleen marginal metallophilic macrophages and in lymph nodes and bone marrow.
    • Product Type
      Monoclonal Antibody
    • Clone
      3D6.112
    • Isotype
      IgG2a
    3 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA884GAC *, F, IFdatasheet pdfdatasheet pdf0.1 mg
      MCA884GA
      MCA884FFdatasheet pdfdatasheet pdf0.1 mg
      MCA884F
      MCA884C *, F, IFdatasheet pdfdatasheet pdf0.2 mg
      MCA884
      MCA884ELC *, F, IFdatasheet pdfdatasheet pdf0.5 mg
      MCA884EL
      MCA884FTFdatasheet pdfdatasheet pdf25 µg
      MCA884FT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180 kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crocker et al. 1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morris et al. 1991).

        CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al. 1992) and granulocytes (Crocker et al. 1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umansky et al. 1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputte et al. 2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wu et al. 2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni (Klass et al. 2012) and group B Streptococcus (Chang et al. 2014).

        The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreux et al. 2008).
      • Intended Use
      • Target Species
        Mouse
      • Product Form
        Purified IgG - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      • Preservative Stabilisers
        0.09%Sodium Azide
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        None present
        0.09%Sodium Azide
        0.09%Sodium Azide
        1%Bovine Serum Albumin
      • Immunogen
        Purified murine sialoadhesin.
      • Purity
      • Approx. Protein Concentrations
        Pack Size: 0.1 mgIgG concentration 1 mg/ml
        Pack Size: 0.2 mgIgG concentration 1.0 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 1 mg/ml
        Pack Size: 0.1 mgIgG concentration 1 mg/ml
        Pack Size: 0.2 mgIgG concentration 1.0 mg/ml
        IgG concentration 0.1 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Fusion Partners
        Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.
      • Storage
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at -20oC only.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
      • GO Terms
      • UniProt
      • Entrez Gene
      • Acknowledgements
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/1001/1000
        Immunofluorescence
        Immunohistology - Frozen(1)1/501/100
        (1)
        Bio-Rad recommend using fixation with either 2% paraformaldehyde or ethanol for optimal results.
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/5
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/1001/1000
        Immunofluorescence
        Immunohistology - Frozen(1)1/501/100
        (1)
        Bio-Rad recommend using fixation with either 2% paraformaldehyde or ethanol for optimal results.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/1001/1000
        Immunofluorescence
        Immunohistology - Frozen(1)1/501/100
        (1)
        Bio-Rad recommend using fixation with either 2% paraformaldehyde or ethanol for optimal results.
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/5

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use

      Additional CD169 Antibody Formats

      Formats Clone Applications Sizes available
      CD169 Antibody : Low Endotoxin 3D6.112 C *, F, IF 0.5 mg
      CD169 Antibody : Purified 3D6.112 C *, F, IF 0.2 mg | 0.1 mg
      CD169 Antibody : FITC 3D6.112 F 25 µg | 0.1 mg
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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        Recommended Negative Isotype Control

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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
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            Mouse Seroblock FcRBUF041B0.5 mgF
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            Recommended Positive Controls

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                References

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