CD45R antibody | RA3-6B2

Rat anti Mouse CD45R:StarBright Violet 440

Product Type
Monoclonal Antibody
Clone
RA3-6B2
Isotype
IgG2a
Specificity
CD45R

Product Code Applications Pack Size List Price Your Price Qty
MCA1258SBV440
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F 100 Tests/0.5ml loader
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Rat anti Mouse CD45R antibody, clone RA3-6B2 recognizes murine CD45R, a form of the CD45 antigen expressed by B cells and lytically active subsets of NK cells and non-MHC restricted CTL's. Rat anti Mouse CD45R antibody, clone RA3-6B2 immunoprecipitates the high molecular weight form of CD45 (220 kDa).

Rat anti Mouse CD45R antibody, clone RA3-6B2 is suitable for plp fixed paraffin embedded tissues (Whiteland et al.1995).

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Cat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to StarBright Violet 440 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
Immunogen
Murine leukemia-induced pre-B tumor cells (RAW112)
Fusion Partners
Spleen cells from immunized Lewis rats were fused with cells of the rat S194/5 XX0.BU-1 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 440 385 438
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
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Mouse Seroblock FcR BUF041B F 0.5 mg loader
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Description Mouse Seroblock FcR

References for CD45R antibody

  1. Monteith, C.E. et al. (1996) Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues.
    Can J Vet Res. 60 (3): 193-8.
  2. Shulga-Morskaya, S. et al. (2004) B cell-activating factor belonging to the TNF family acts through separate receptors to support B cell survival and T cell-independent antibody formation.
    J Immunol. 173 (4): 2331-41.
  3. McGill, J. et al. (2009) Fetal exposure to ethanol has long-term effects on the severity of influenza virus infections.
    J Immunol. 182: 7803-8
  4. Straubinger, R.K. et al. (2003) Quantitative evaluation of inflammatory and immune responses in the early stages of chronic Helicobacter pylori infection.
    Infect Immun. 71: 2693-703.
  5. Itoh, T. et al. (2007) Ddb2 is a haploinsufficient tumor suppressor and controls spontaneous germ cell apoptosis.
    Hum Mol Genet. 16: 1578-86.
  6. Zhou, Z. et al. (2011) Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.
    BMC Immunol. 2011; 12:7.
  7. Allen, A.C. et al. (2021) Parallel in vivo. experimental evolution reveals that increased stress resistance was key for the emergence of persistent tuberculosis bacilli.
    Nat Microbiol. 6 (8): 1082-93.
  8. Coffman, R.L. (1982) Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development.
    Immunol Rev. 69: 5-23.
  9. View The Latest Product References
  10. Rosmalen, J.G. et al. (2000) Subsets of macrophages and dendritic cells in nonobese diabetic mouse pancreatic inflammatory infiltrates: correlation with the development of diabetes.
    Lab Invest. 80 (1): 23-30.
  11. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  12. Spangrude, G.J. et al. (1988) Purification and characterization of mouse hematopoietic stem cells.
    Science. 241: 58-62.
  13. Spangrude, G.J. et al. (1988) Two rare populations of mouse Thy-1lo bone marrow cells repopulate the thymus.
    J Exp Med. 167 (5): 1671-83.
  14. Holmes, K.L. et al. (1986) Analysis of neoplasms induced by Cas-Br-M MuLV tumor extracts.
    J Immunol. 137 (2): 679-88.
  15. Ankeny, D.P. et al. (2009) B cells produce pathogenic antibodies and impair recovery after spinal cord injury in mice.
    J Clin Invest. 119: 2990-9.
  16. Lundqvist, J. et al. (2010) Concomitant infection decreases the malaria burden but escalates relapsing fever borreliosis.
    Infect Immun. 78 (5): 1924-30.
  17. Herrmann, I. et al. (2006) Streptococcus pneumoniae Infection aggravates experimental autoimmune encephalomyelitis via Toll-like receptor 2.
    Infect Immun. 74: 4841-8.
  18. Kleiter, I. et al. (2010) Smad7 in T cells drives T helper 1 responses in multiple sclerosis and experimental autoimmune encephalomyelitis.
    Brain. 133: 1067-81.
  19. Lacroix-Lamande, S. et al. (2009) Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation.
    PLoS One. 4: e8291.
  20. Bertilaccio, M.T. et al. (2011) Lack of TIR8/SIGIRR triggers progression of chronic lymphocytic leukemia in mouse models.
    Blood. 118: 660-9.
  21. Gengozian, N. et al. (2005) Characterization of a monoclonal antibody identifying a CD45RA antigen on feline leukocytes.
    Vet Immunol Immunopathol. 108: 253-64.
  22. Giuriato, S. et al. (2010) Conditional TPM3-ALK and NPM-ALK transgenic mice develop reversible ALK-positive early B-cell lymphoma/leukemia.
    Blood. 115: 4061-70.
  23. Hawke, S. et al. (1998) Long-term persistence of activated cytotoxic T lymphocytes after viral infection of the central nervous system.
    J Exp Med. 187: 1575-82.
  24. Nakaya, T. et al. (2010) Critical role of Pcid2 in B cell survival through the regulation of MAD2 expression.
    J Immunol. 185: 5180-7.
  25. Perry, M.J. et al. (2000) Effects of high-dose estrogen on murine hematopoietic bone marrow precede those on osteogenesis.
    Am J Physiol Endocrinol Metab. 279: E1159-65.
  26. Gibson-Corley, K.N. et al. (2016) A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis.
    PeerJ. 4: e1600.
  27. Soejima, M. et al. (2011) Role of innate immunity in a murine model of histidyl-transfer RNA synthetase (Jo-1)-mediated myositis.
    Arthritis Rheum. 63: 479-87.
  28. Stevenson, P.G. et al. (2002) Uncoupling of virus-induced inflammation and anti-viral immunity in the brain parenchyma.
    J Gen Virol. 83: 1735-43.
  29. Fanning, S. et al. (2012) Bifidobacterial surface-exopolysaccharide facilitates commensal-host interaction through immune modulation and pathogen protection.
    Proc Natl Acad Sci U S A. 109 (6): 2108-13.
  30. Ruf, M.T. et al. (2012) Chemotherapy-Associated Changes of Histopathological Features of Mycobacterium ulcerans Lesions in a Buruli Ulcer Mouse Model.
    Antimicrob Agents Chemother. 56: 687-96.
  31. Carpenter, R.S. et al. (2015) Traumatic spinal cord injury in mice with human immune systems.
    Exp Neurol. 271: 432-44.
  32. Lastrucci, C. et al. (2015) Molecular and cellular profiles of the resolution phase in a damage-associated molecular pattern (DAMP)-mediated peritonitis model and revelation of leukocyte persistence in peritoneal tissues.
    FASEB J. 29 (5): 1914-29.
  33. Thiele, L.S.N. et al. (2020) Functional relevance of the multi-drug transporter abcg2 on teriflunomide therapy in an animal model of multiple sclerosis.
    J Neuroinflammation. 17 (1): 9.
  34. Jaensch, S. et al. (2022) Clinicopathologic and immunophenotypic features in dogs with presumptive large granular lymphocyte leukaemia
    Aus Vet J. Aug 12 [Epub ahead of print].

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Synonyms
B220
LY-5
UniProt
P06800
Entrez Gene
Ptprc
GO Terms
GO:0000187 activation of MAPK activity
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
GO:0051726 regulation of cell cycle
GO:0004725 protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007159 leukocyte cell-cell adhesion
GO:0008201 heparin binding
GO:0009897 external side of plasma membrane
GO:0019901 protein kinase binding
GO:0030183 B cell differentiation
GO:0030890 positive regulation of B cell proliferation
GO:0031953 negative regulation of protein autophosphorylation
GO:0034113 heterotypic cell-cell adhesion
GO:0042098 T cell proliferation
GO:0042100 B cell proliferation
GO:0043065 positive regulation of apoptosis
GO:0043395 heparan sulfate proteoglycan binding
GO:0043410 positive regulation of MAPKKK cascade
GO:0045059 positive thymic T cell selection
GO:0045060 negative thymic T cell selection
GO:0045121 membrane raft
GO:0045577 regulation of B cell differentiation
GO:0045588 positive regulation of gamma-delta T cell differentiation
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050855 regulation of B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
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