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Respiratory Syncytial Virus Fusion Protein antibody | RSV3216 (B016)

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Mouse anti Respiratory Syncytial Virus Fusion Protein

Product Type
Monoclonal Antibody
Clone
RSV3216 (B016)
Isotype
IgG2b
Specificity
Respiratory Syncytial Virus Fusion Protein

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Product Code Applications Pack Size List Price Your Price Qty
MCA490
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E * EM F IF IP WB 1 mg
List Price Your Price

Mouse anti respiratory syncytial virus fusion protein antibody, clone RSV3216 recognizes an epitope within the RSV fusion protein (46 kDa and 22 kDa s-s linked glycoprotein).

Mouse anti respiratory syncytial virus fusion protein antibody, clone RSV3216 can be used in immunofluorescence assays in conjunction with MCA491G (clone RSV3132).

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Bovine RSV strains: 127, SNK and 9007. Human RSV strains: Long, Randall, 8/60, and A/2.
Purity
>90% IgG
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1
Flow Cytometry
Immuno-electron Microscopy
Immunofluorescence
Immunoprecipitation
Western Blotting
  1. 1Suitable as capture reagent with MCA491 as detection reagent in sandwich ELISA (See Adams et al. 2010 for details).
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for Respiratory Syncytial Virus Fusion Protein antibody

  1. Sandhu, J.S. et al. (2000) Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response.
    Transgenic Res. 9 (2): 127-35.
  2. Zimmer, G. et al. (2001) N-glycans of F protein differentially affect fusion activity of human respiratory syncytial virus.
    J Virol. 75 (10): 4744-51.
  3. Zimmer, G. et al. (2001) Proteolytic activation of respiratory syncytial virus fusion protein. Cleavage at two furin consensus sequences.
    J Biol Chem. 276 (34): 31642-50.
  4. Zimmer, G. et al. (2002) Cleavage at the furin consensus sequence RAR/KR(109) and presence of the intervening peptide of the respiratory syncytial virus fusion protein are dispensable for virus replication in cell culture.
    J Virol. 76 (18): 9218-24.
  5. Zimmer, G. et al. (2003) Virokinin, a bioactive peptide of the tachykinin family, is released from the fusion protein of bovine respiratory syncytial virus.
    J Biol Chem. 278: 46854-61.
  6. Schlender, J. et al. (2003) Respiratory syncytial virus (RSV) fusion protein subunit F2, not attachment protein G, determines the specificity of RSV infection.
    J Virol. 77: 4609-16.
  7. Mason, S.W. et al. (2004) Polyadenylation-dependent screening assay for respiratory syncytial virus RNA transcriptase activity and identification of an inhibitor.
    Nucleic Acids Res. 32 (16): 4758-67.
  8. Zimmer, G. et al. (2005) A chimeric respiratory syncytial virus fusion protein functionally replaces the F and HN glycoproteins in recombinant Sendai virus.
    J Virol. 79: 10467-77.
    9. View The Latest Product References
  9. Schlender, J. et al. (2005) Inhibition of toll-like receptor 7- and 9-mediated alpha/beta interferon production in human plasmacytoid dendritic cells by respiratory syncytial virus and measles virus.
    J Virol. 79 (9): 5507-15.
  10. Riffault, S. et al. (2006) Replication of bovine respiratory syncytial virus in murine cells depends on type I interferon-receptor functionality.
    J Gen Virol. 87: 2145-8.
  11. Fleming, E.H. et al. (2006) Respiratory syncytial virus F envelope protein associates with lipid rafts without a requirement for other virus proteins.
    J Virol. 80 (24): 12160-70.
  12. Kolokoltsov, A.A. et al. (2007) Small interfering RNA profiling reveals key role of clathrin-mediated endocytosis and early endosome formation for infection by respiratory syncytial virus.
    J Virol. 81: 7786-800.
  13. Eckardt-Michel, J. et al. (2008) The fusion protein of respiratory syncytial virus triggers p53-dependent apoptosis.
    J Virol. 82 (7): 3236-49.
  14. Riffault, S. et al. (2010) A new subunit vaccine based on nucleoprotein nanoparticles confers partial clinical and virological protection in calves against bovine respiratory syncytial virus.
    Vaccine. 28: 3722-34.
  15. Yunus, A. et al. (2010) Elevated temperature triggers human respiratory syncytial virus F protein six-helix bundle formation.
    Virology. 396: 226-37.
  16. Adams, O. et al. (2010) Palivizumab-resistant human respiratory syncytial virus infection in infancy.
    Clin Infect Dis. 2010 Jul 15;51(2):185-8.
  17. Shaw, C.A. et al. (2013) The role of non-viral antigens in the cotton rat model of respiratory syncytial virus vaccine-enhanced disease.
    Vaccine. 31 (2): 306-12.
  18. Adams, O. et al. (2013) Genetic analysis and antigenic characterization of human respiratory syncytial virus group A viruses isolated in Germany 1996-2008.
    Virus Genes. 47 (2): 210-8.
  19. Hägglund S et al. (2014) Characterization of an experimental vaccine for bovine respiratory syncytial virus.
    Clin Vaccine Immunol. 21 (7): 997-1004.
  20. Wong TM et al. (2014) Respiratory syncytial virus (RSV) infection in elderly mice results in altered antiviral gene expression and enhanced pathology.
    PLoS One. 9 (2): e88764.
  21. Bird, G.H. et al. (2014) Mucosal delivery of a double-stapled RSV peptide prevents nasopulmonary infection.
    J Clin Invest. 124 (5): 2113-24.
  22. Hervé, P.L. et al. (2017) RSV N-nanorings fused to palivizumab-targeted neutralizing epitope as a nanoparticle RSV vaccine.
    Nanomedicine. 13 (2): 411-20.
  23. Sampayo-Escobar, V. et al. (2018) Osteopontin plays a pivotal role in increasing severity of respiratory syncytial virus infection.
    PLoS One. 13 (4): e0192709.
  24. Koskinen, J.M. et al. (2021) Clinical validation of automated and rapid mariPOC SARS-CoV-2 antigen test.
    Sci Rep. 11 (1): 20363.

Flow Cytometry

Immuno-electron Microscopy

Immunofluorescence

Immunoprecipitation

Western Blotting

Synonyms
RSV
RRID
AB_2231368

MCA490

150338 154890 157371 163453

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