Influenza B Nucleoprotein antibody | B017 (B35G)

Mouse anti Influenza B Nucleoprotein

Product Type
Monoclonal Antibody
Clone
B017 (B35G)
Isotype
IgG2b
Specificity
Influenza B Nucleoprotein

Product Code Applications Pack Size List Price Your Price Qty
MCA403
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IF WB 1 mg loader
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loader
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Mouse anti Influenza B Nucleoprotein antibody, clone B017 recognises an epitope within the nucleoprotein of influenza B virus.

The influenza viruses, classified as type A, B and C, are members of the Orthomyxoviridae family which differ in their epidemiology and host ranges and lack serological cross-reactivity of their internal components, especially their matrix proteins and nucleoprotein. Influenza B virus is a slow-mutating single stranded RNA virus subject to antigenic drift which, although enough to prevent lasting immunity, prevents influenza B from causing pandemics.

Mouse anti Influenza B Nucleoprotein antibody, clone B017 can be used in influenza B IFA typing in conjunction with MCA2717 (clone B114).

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Influenza B/Lee/40 and B/Singapore/-222/79 viruses.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3 Ag8.653 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Influenza B Nucleoprotein antibody

  1. Walls, H.H. et al. (1986) Characterization and evaluation of monoclonal antibodies developed for typing influenza A and influenza B viruses.
    J Clin Microbiol. 23 (2): 240-5.
  2. Zhirnov, O.P. et al. (1999) Caspase-dependent N-terminal cleavage of influenza virus nucleocapsid protein in infected cells.
    J Virol. 73 (12): 10158-63.
  3. Ehrhardt, C. et al. (2007) Activation of phosphatidylinositol 3-kinase signaling by the nonstructural NS1 protein is not conserved among type A and B influenza viruses.
    J Virol. 81 (21): 12097-100.
  4. Goujon, C. & Malim, M.H. (2010) Characterization of the alpha interferon-induced postentry block to HIV-1 infection in primary human macrophages and T cells.
    J Virol. 84 (18): 9254-66.
  5. Dauber, B. et al. (2009) Influenza B virus ribonucleoprotein is a potent activator of the antiviral kinase PKR.
    PLoS Pathog. 5 (6): e1000473.
  6. Lesch, M. et al. (2019) RNAi-based small molecule repositioning reveals clinically approved urea-based kinase inhibitors as broadly active antivirals
    PLoS Pathog. 15(3):e1007601.

Flow Cytometry

Western Blotting

RRID
AB_2298473
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