Influenza A Nucleoprotein antibody | AA5H

Mouse anti Influenza A Nucleoprotein

Product Type
Monoclonal Antibody
Clone
AA5H
Isotype
IgG2a
Specificity
Influenza A Nucleoprotein

Product Code Applications Pack Size List Price Your Price Qty
MCA400
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF P WB 1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Influenza A Nucleoprotein antibody, clone AA5H recognizes an epitope within Influenza virus A nucleoprotein. Mouse anti Influenza A Nucleoprotein antibody, clone AA5H can be used in influenza A IFA typing in conjunction with MCA401 (clone GA2B).

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Influenza A / Puerto Rico / 8 / 34 (H1N1) and A/Bangkok / 1 / 79 (H3N2) viruses.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells of the P3 Ag8.653 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Paraffin
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Influenza A Nucleoprotein antibody

  1. Herold, S. et al. (2006) Alveolar epithelial cells direct monocyte transepithelial migration upon influenza virus infection: impact of chemokines and adhesion molecules.
    J Immunol. 177 (3): 1817-24.
  2. Ehrhardt, C. et al. (2007) Influenza A virus NS1 protein activates the PI3K/Akt pathway to mediate antiapoptotic signaling responses.
    J Virol. 81: 3058-67.
  3. Ehrhardt, C. et al. (2007) Activation of phosphatidylinositol 3-kinase signaling by the nonstructural NS1 protein is not conserved among type A and B influenza viruses.
    J Virol. 81: 12097-100.
  4. Matarrese, P. et al. (2011) Pepstatin A alters host cell autophagic machinery and leads to a decrease in influenza A virus production.
    J Cell Physiol. 226 (12): 3368-77.
  5. Nencioni, L. et al. (2009) Bcl-2 expression and p38MAPK activity in cells infected with influenza A virus: impact on virally induced apoptosis and viral replication.
    J Biol Chem. 284: 16004-15.
  6. Pauli, E.K. et al. (2008) Influenza A virus inhibits type I IFN signaling via NF-kappaB-dependent induction of SOCS-3 expression.
    PLoS Pathog. 4(11): e1000196.
  7. Jamali, A. et al. (2010) A DNA vaccine-encoded nucleoprotein of influenza virus fails to induce cellular immune responses in a diabetic mouse model.
    Clin Vaccine Immunol. 17: 683-7.
  8. Ehrhardt, C. et al. (2007) A polyphenol rich plant extract, CYSTUS052, exerts anti influenza virus activity in cell culture without toxic side effects or the tendency to induce viral resistance.
    Antiviral Res. 76: 38-47.
  9. View The Latest Product References
  10. Seitz, C. et al. (2010) High yields of influenza A virus in Madin-Darby canine kidney cells are promoted by an insufficient interferon-induced antiviral state.
    J Gen Virol. 91: 1754-63.
  11. Gabay, C. et al. (2011) Impact of synthetic and biologic disease-modifying antirheumatic drugs on antibody responses to the AS03-adjuvanted pandemic influenza vaccine: a prospective, open-label, parallel-cohort, single-center study.
    Arthritis Rheum. 63 (6): 1486-96.
  12. Luig, C. et al. (2010) MAP kinase-activated protein kinases 2 and 3 are required for influenza A virus propagation and act via inhibition of PKR.
    FASEB J. 24: 4068-77.
  13. Shu, Y. et al. (2010) Avian influenza A(H5N1) viruses can directly infect and replicate in human gut tissues.
    J Infect Dis. 201: 1173-7.
  14. Hassan, I.H. et al. (2012) Influenza A viral replication is blocked by inhibition of the inositol-requiring enzyme 1 (IRE1) stress pathway.
    J Biol Chem. 287 (7): 4679-89.
  15. Brnic, D. et al. (2012) Borna disease virus infects human neural progenitor cells and impairs neurogenesis.
    J Virol. 86 (5): 2512-22.
  16. Hrincius, E.R. et al. (2011) Phosphatidylinositol-3-kinase (PI3K) is activated by influenza virus vRNA via the pathogen pattern receptor Rig-I to promote efficient type I interferon production.
    Cell Microbiol. 13: 1907-19.
  17. Koerner, I. et al. (2012) Altered receptor specificity and fusion activity of the haemagglutinin contribute to high virulence of a mouse-adapted influenza A virus.
    J Gen Virol. 93 (Pt 5): 970-9.
  18. Thompson, C.I. et al. (2006) Infection of human airway epithelium by human and avian strains of influenza a virus.
    J Virol. 80: 8060-8.
  19. Gao, R. et al. (2010) A systematic molecular pathology study of a laboratory confirmed H5N1 human case.
    PLoS One. 5: e13315.
  20. Matthaei M et al. (2013) Highly pathogenic H5N1 influenza A virus strains provoke heterogeneous IFN-α/β responses that distinctively affect viral propagation in human cells.
    PLoS One. 8 (2): e56659.
  21. Wörmann, X. et al. (2016) Genetic characterization of an adapted pandemic 2009 H1N1 influenza virus that reveals improved replication rates in human lung epithelial cells
    Virology. 492: 118-29.
  22. Sadewasser, A. et al. (2017) Quantitative proteomic approach identifies Vpr binding protein as novel host factor supporting influenza A virus infections in human cells.
    Mol Cell Proteomics. pii: mcp.M116.065904. [Epub ahead of print]
  23. Kim HR et al. (2016) Ostrich ( Struthio camelus ) Infected with H5N8 Highly Pathogenic Avian Influenza Virus in South Korea in 2014.
    Avian Dis. 60 (2): 535-9.
  24. Dick, A. et al. (2015) Role of nucleotide binding and GTPase domain dimerization in dynamin-like myxovirus resistance protein A for GTPase activation and antiviral activity.
    J Biol Chem. 290 (20): 12779-92.
  25. Shoji, M. et al. (2015) Bakuchiol Is a Phenolic Isoprenoid with Novel Enantiomer-selective Anti-influenza A Virus Activity Involving Nrf2 Activation.
    J Biol Chem. 290 (46): 28001-17.
  26. Thulasi Raman, S.N. et al. (2016) DDX3 Interacts with Influenza A Virus NS1 and NP Proteins and Exerts Antiviral Function through Regulation of Stress Granule Formation.
    J Virol. 90 (7): 3661-75.
  27. Youchan, B. et al. (2018) Pathological lesions and antigen localization in chicken, ducks and Japanese quail naturally infected by novel highly pathogenic avian influenza (H5N6), Korea, 2016
    J Prev Vet Med. 42 (3): 91-8.
  28. Sid, H. et al. (2017) Interaction of Influenza A Viruses with Oviduct Explants of Different Avian Species.
    Front Microbiol. 8: 1338.
  29. Prokopyeva, E.A. et al. (2019) Pathology of A(H5N8) (Clade 2.3.4.4) Virus in Experimentally Infected Chickens and Mice.
    Interdiscip Perspect Infect Dis. 2019: 4124865.
  30. Calmy, A. et al. (2012) Strong serological responses and HIV RNA increase following AS03-adjuvanted pandemic immunization in HIV-infected patients.
    HIV Med. 13 (4): 207-18.
  31. Mayr, J. et al. (2018) Unravelling the Role of O-glycans in Influenza A Virus Infection.
    Sci Rep. 8 (1): 16382.
  32. MacKerracher, A. et al. (2022) PLGA particle vaccination elicits resident memory CD8 T cells protecting from tumors and infection.
    Eur J Pharm Sci. : 106209.

Immunofluorescence

Immunohistology - Paraffin

Immunoprecipitation

Western Blotting

RRID
AB_2151884
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