Influenza A Nucleoprotein antibody | 1341
Mouse anti Influenza A nucleoprotein antibody, clone 1341 (OBT0846) used for the identification of influenza protein in tracheal organ cultures by immunohistochemistry acetone fixed cryosections.
Image caption:
Detection of viral antigen (A,B,C) and induction of apoptosis (E,F,G) in TOC-Ch after infection with Ch/H9N2. TOC-Ch were infected with 104 PFU of Ch/H9N2 in the third consecutive passage or with PBS as non-infected control. Cryosections of TOC were used for immunohistochemical staining for viral nucleoprotein (A,B,C). (A) Non-infected TOC-Ch at 8 hpi, (B) Ch/H9N2-infected TOC-Ch at 8 hpi, (C) Ch/H9N2-infected TOC-Ch at 24 hpi. Non-infected TOC-Ch were negative for viral antigen staining at 24 hpi. In situ TUNEL staining for apoptosis (D,E,F). (D) Non-infected TOC-Ch at 24 hpi (no difference to non-infected TOC-Ch at 8 hpi), (E) Ch/H9N2-infected TOC-Ch at 8 hpi, (F) Ch/H9N2-infected TOC-Ch at 24 hpi. Brown staining indicates the presence of viral antigen (A,B,C) or apoptotic cells (D,E,F).
From: Petersen H, Matrosovich M, Pleschka S, Rautenschlein S (2012)
Replication and Adaptive Mutations of Low Pathogenic Avian Influenza Viruses in Tracheal Organ Cultures of Different Avian Species.
PLoS ONE 7(8): e42260.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Influenza A Nucleoprotein
- Product Type
- Monoclonal Antibody
- Clone
- 1341
- Isotype
- IgG2a
- Specificity
- Influenza A Nucleoprotein
Antibody Quality and Performance Guarantee
| Mouse anti Influenza A nucleoprotein antibody, clone 1341 Influenza A nucleoprotein. Mouse anti Influenza A nucleoprotein antibody, clone 1341 reacts against all strains of Influenza A. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Partially purified Influenza A.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/20 | 1/200 |
| Immunofluorescence | |
1/10 | 1/50 |
| Immunohistology - Frozen 1 | |
1/10 | 1/50 |
| Immunohistology - Paraffin |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for Influenza A Nucleoprotein antibody
-
Liang, Y. et al. (2005) cis-Acting packaging signals in the influenza virus PB1, PB2, and PA genomic RNA segments.
J Virol. 79: 10348-55. -
Petersen, H. et al. (2012) Replication and adaptive mutations of low pathogenic avian influenza viruses in tracheal organ cultures of different avian species.
PLoS One. 7: e42260. -
Sid, H. et al. (2016) Mycoplasma gallisepticum modifies the pathogenesis of influenza A virus in the avian tracheal epithelium.
Int J Med Microbiol. 306 (3): 174-86.
- RRID
- AB_2298472
OBT0846
111113
156977
159661
165026
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