Influenza A antibody | 1331
Mouse anti Influenza A nucleoprotein antibody, clone 1331 (0300-0234) used to visualize internalized virus by immunofluorescence on infected MDCK cells
Image caption:
Effect of iota-carrageenan on H1N1 virus adsorption and internalization.
(A) Adsorption. H1N1 virus was added to MDCK cells in the presence of different concentrations of iota-carrageenan or control polymer carboxymethylcellulose (CMC). After viral adsorption for 1 h at 4°C, cells were washed and the number of cell-bound infectious viral particles determined by plaque assay; red bar 400 μg/ml, orange bar 4 μg/ml iota-carrageenan, black bar 400 μg/ml, grey bar 4 μg/ml CMC. (B) Adsorption/Internalization. H1N1 virus was added to MDCK cells and adsorbed for 1 h at 4°C. Cells were washed and allowed to internalize virus in the presence or absence of different concentrations of iota-carrageenan or CMC for 1 h at 37°C. Subsequently, internalized infectious viral particles were determined by plaque assay. (C) Immunofluorescent visualisation of virus adsorption in the presence of iota-carrageenan or CMC. 1 h post adsorption at 4°C, cells were stained after 1 h at 37°C with a mouse anti-NP antibody. (D) Adsorption/Internalization. H1N1 was added to MDCK cells and adsorbed for 1 h at 4°C. Cells were washed and allowed to internalize virus in the presence of iota-carrageenan or CMC for 1 h at 37°C. Compare the bright green stainings in indicative of productive infection to 5C, where no green fluorescence is detected at high iota-carrageenan concentration.
From: Leibbrandt A, Meier C, König-Schuster M, Weinmüllner R, Kalthoff D, Pflugfelder B, et al. (2010)
Iota-Carrageenan Is a Potent Inhibitor of Influenza A Virus Infection.
PLoS ONE 5(12): e14320.
Mouse anti Influenza A
- Product Type
- Monoclonal Antibody
- Clone
- 1331
- Isotype
- IgG2a
- Specificity
- Influenza A
Antibody Quality and Performance Guarantee
| Mouse anti Influenza A antibody, clone 1331, recognizes the nucleoprotein of Influenza A. It has been found reactive with over 50 isolates from H1 through to H14 and gives 'nuclear' staining in immunofluorescence. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Influenza virus from Texas strain.
- Approx. Protein Concentrations
- 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/200 | 1/2000 |
| Immunofluorescence | |
1/100 | 1/500 |
| Immunohistology - Frozen | |
1/100 | 1/500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
References for Influenza A antibody
-
Leibbrandt, A. et al. (2010) Iota-carrageenan is a potent inhibitor of influenza A virus infection.
PLoS One. 5: e14320. -
Nakayama, M. et al. (2016) Vaccination against H9N2 avian influenza virus reduces bronchus-associated lymphoid tissue formation in cynomolgus macaques after intranasal virus challenge infection.
Pathol Int. 66 (12): 678-86.
- RRID
- AB_620584
- UniProt
- P18072
- GO Terms
- GO:0003723 RNA binding
- GO:0030529 ribonucleoprotein complex
- GO:0005198 structural molecule activity
- GO:0019012 virion
- GO:0042025 host cell nucleus
- GO:0044419 interspecies interaction between organisms
0300-0234
160707
160711
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