Hepatitis B X Antigen antibody | 3F6-G10

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Mouse anti Hepatitis B X Antigen

Product Type
Monoclonal Antibody
Clone
3F6-G10
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1391 C E P 0.25 mg
Mouse anti Hepatitis B-X antibody, clone 3F6-G10 recognizes the HB-X antigen of hepatitis virus. The Hepatitis B X antigen is a 154 amino acid ~17 kDa multifunctional protein involved in the development of liver chirrosis and hepatocellular carcinoma (UniProt: P03165).

Mouse anti Hepatitis B-X antibody, clone 3F6-G10 was produced by immunization of mice with a "HB-X- Protein A" fusion construct and subsequent screening of hybridoma products against a "HB-X-GST" fusion construct (Marczinovits et al. 1997).

Mouse anti Hepatitis B-X antibody, clone 3F6-G10 has been used succesfully for the detection of the hepatitis B X antigen by immunohistochemistry in formalin fixed, paraffin embedded material, also by western blotting against the immunizing and screening fusion proteins (Pál et al. 2001). Subsequently clone 3F6-G10 has been used as a capture reagent in a sensitive sandwich ELISA and bead based flow assay for the quantitative assessment of HbX antigen in human sera (Pál et al. 2005).

Fine epitope mapping by phage library screening indicates that the epitope recognized by Mouse anti Hepatitis B-X antibody, clone 3F6-G10 lies between amino acids 88 and 93 of the X antigen, a result subsequently confirmed by peptide ELISA (Pál et al. 2003).

Product Details

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Preservative Stabilisers
0.09%Sodium Azide
0.1%Bovine Serum Albumin
Immunogen
HB-Xag-Protein A Fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of a mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P03165 Related reagents
GO Terms
GO:0006915 apoptosis
GO:0006350 transcription
GO:0019079 viral genome replication
GO:0033650 host cell mitochondrion
GO:0042025 host cell nucleus
GO:0044419 interspecies interaction between organisms
GO:0045449 regulation of transcription
Regulatory
For research purposes only

Applications of Hepatitis B X Antigen antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen 1/100
Immunohistology - Paraffin 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Liver Carcinoma/Hepatitis B infected liver.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Hepatitis B X Antigen antibody

  1. Pál, J. et al. (2001) Immunohistochemical assessment and prognostic value of hepatitis B virus X protein in chronic hepatitis and primary hepatocellular carcinomas using anti-HBxAg monoclonal antibody.
    Pathol Oncol Res. 7: 178-84.
  2. Lei, J.H. et al (2007) Effects of HBV X gene and arsenic trioxide on the expression of p53 in cultured HepG2 cells.
    Chin Med J 120: 2181-4.
  3. Pál, J. et al. (2003) Determination of the fine epitope specificity of an anti-hepatitis B virus X protein monoclonal antibody using microanalytical and molecular biological methods.
    Mol Immunol. 40: 241-6.
  4. Pál, J. et al. (2005) Sandwich type ELISA and a fluorescent cytometric microbead assay for quantitative determination of hepatitis B virus X antigen level in human sera.
    J Immunol Methods. 306: 183-92.
  5. Cheng, P. et al. (2009) Hepatitis B virus X protein (HBx) induces G2/M arrest and apoptosis through sustained activation of cyclin B1-CDK1 kinase
    Oncol Rep. 22: 1101-7.
  6. Chun, E. et al. (2003) Tumor eradication by hepatitis B virus X antigen-specific CD8+ T cells in xenografted nude mice.
    J Immunol. 170: 1183-90.
  7. Pál, J. et al. (2006) Comprehensive regression analysis of hepatitis B virus X antigen level and anti-HBx antibody titer in the sera of patients with HBV infection.
    Pathol Oncol Res. 12: 34-40.