Hepatitis B X Antigen antibody | 3F6-G10
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Mouse anti Hepatitis B-X antibody, clone 3F6-G10 recognizes the HB-X antigen of hepatitis virus. The Hepatitis B X antigen is a 154 amino acid ~17 kDa multifunctional protein involved in the development of liver chirrosis and hepatocellular carcinoma (UniProt: P03165).
Mouse anti Hepatitis B-X antibody, clone 3F6-G10 was produced by immunization of mice with a "HB-X- Protein A" fusion construct and subsequent screening of hybridoma products against a "HB-X-GST" fusion construct (Marczinovits et al. 1997).
Mouse anti Hepatitis B-X antibody, clone 3F6-G10 has been used succesfully for the detection of the hepatitis B X antigen by immunohistochemistry in formalin fixed, paraffin embedded material, also by western blotting against the immunizing and screening fusion proteins (Pál et al. 2001). Subsequently clone 3F6-G10 has been used as a capture reagent in a sensitive sandwich ELISA and bead based flow assay for the quantitative assessment of HbX antigen in human sera (Pál et al. 2005).
Fine epitope mapping by phage library screening indicates that the epitope recognized by Mouse anti Hepatitis B-X antibody, clone 3F6-G10 lies between amino acids 88 and 93 of the X antigen, a result subsequently confirmed by peptide ELISA (Pál et al. 2003).
Mouse anti Hepatitis B-X antibody, clone 3F6-G10 was produced by immunization of mice with a "HB-X- Protein A" fusion construct and subsequent screening of hybridoma products against a "HB-X-GST" fusion construct (Marczinovits et al. 1997).
Mouse anti Hepatitis B-X antibody, clone 3F6-G10 has been used succesfully for the detection of the hepatitis B X antigen by immunohistochemistry in formalin fixed, paraffin embedded material, also by western blotting against the immunizing and screening fusion proteins (Pál et al. 2001). Subsequently clone 3F6-G10 has been used as a capture reagent in a sensitive sandwich ELISA and bead based flow assay for the quantitative assessment of HbX antigen in human sera (Pál et al. 2005).
Fine epitope mapping by phage library screening indicates that the epitope recognized by Mouse anti Hepatitis B-X antibody, clone 3F6-G10 lies between amino acids 88 and 93 of the X antigen, a result subsequently confirmed by peptide ELISA (Pál et al. 2003).
Product Details
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.1% Bovine Serum Albumin - Immunogen
- HB-Xag-Protein A Fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the Sp-2/0-Ag14 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P03165
- GO Terms
- GO:0006915 apoptosis
- GO:0006350 transcription
- GO:0019079 viral genome replication
- GO:0033650 host cell mitochondrion
- GO:0042025 host cell nucleus
- GO:0044419 interspecies interaction between organisms
- GO:0045449 regulation of transcription
- Regulatory
- For research purposes only
Applications of Hepatitis B X Antigen antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Frozen | 1/100 | ||
| Immunohistology - Paraffin | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Liver Carcinoma/Hepatitis B infected liver.
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Secondary Antibodies Available
Product Specific References
References for Hepatitis B X Antigen antibody
-
Somogyi, C.S. et al. (1995) Development and characterization of polyclonal and monoclonal antibodies against HBXAg
Progress in Clinical Virology Joint Meeting, p. 209. -
Pál, J. et al. (2001) Immunohistochemical assessment and prognostic value of hepatitis B virus X protein in chronic hepatitis and primary hepatocellular carcinomas using anti-HBxAg monoclonal antibody.
Pathol Oncol Res. 7: 178-84. -
Lei, J.H. et al (2007) Effects of HBV X gene and arsenic trioxide on the expression of p53 in cultured HepG2 cells.
Chin Med J 120: 2181-4. -
Pál, J. et al. (2003) Determination of the fine epitope specificity of an anti-hepatitis B virus X protein monoclonal antibody using microanalytical and molecular biological methods.
Mol Immunol. 40: 241-6. -
Pál, J. et al. (2005) Sandwich type ELISA and a fluorescent cytometric microbead assay for quantitative determination of hepatitis B virus X antigen level in human sera.
J Immunol Methods. 306: 183-92. -
Cheng, P. et al. (2009) Hepatitis B virus X protein (HBx) induces G2/M arrest and apoptosis through sustained activation of cyclin B1-CDK1 kinase
Oncol Rep. 22: 1101-7. -
Chun, E. et al. (2003) Tumor eradication by hepatitis B virus X antigen-specific CD8+ T cells in xenografted nude mice.
J Immunol. 170: 1183-90. -
Pál, J. et al. (2006) Comprehensive regression analysis of hepatitis B virus X antigen level and anti-HBx antibody titer in the sera of patients with HBV infection.
Pathol Oncol Res. 12: 34-40.
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