Feline Leukaemia Virus p27 antibody | PF12J-10A

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Mouse anti Feline Leukaemia Virus p27

Product Type
Monoclonal Antibody
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2551 E P WB 0.25 mg
Mouse anti Feline Leukaemia Virus p27 antibody, clone PF12J-10A recognises feline leukaemia virus (FeLV) p27 core protein (p27).

The oncoretrovirus FeLV is responsible for both neoplastic and non-neoplastic conditions, often resulting in a poor prognosis and fatal outcome for cats persistently infected. During persistent infection the virus, soluble viral antigens and antigen containing white blood cells are continuously present in the blood and diagnosis is mainly based upon detection of the p27 antigen - using ELISA to test for p27 as it circulates free in the bloodstream, and Immunofluorescence studies to detect p27 within the infected white cells.

Product Details

Target Species
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05%Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
18 months from date of despatch.

More Information

O89811 Related reagents
For research purposes only

Applications of Feline Leukaemia Virus p27 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/100
Western Blotting 1/1,500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2551 detects a band of approximately 28kDa under reducing conditions. A strong band of 80kDa representing glycosylated FeLV gag gene product may also be present.

Product Specific References

References for Feline Leukaemia Virus p27 antibody

  1. Kipar, A. et al. (2000) Expression of viral proteins in feline leukemia virus-associated enteritis.
    Vet Pathol. 37 (2): 129-36.
  2. Kipar, A. et al. (2001) Comparative examination of cats with feline leukemia virus-associated enteritis and other relevant forms of feline enteritis.
    Vet Pathol. 38 (4): 359-71.
  3. Kipar, A. et al. (1998) Fatal enteritis associated with coronavirus infection in cats.
    J Comp Pathol. 119 (1): 1-14.
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