Feline Leukaemia Virus p27 antibody | PF12J-10A

Mouse anti Feline Leukaemia Virus p27

Product Type
Monoclonal Antibody
Clone
PF12J-10A
Isotype
IgG1
Specificity
Feline Leukaemia Virus p27

Product Code Applications Pack Size List Price Your Price Qty
MCA2551
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P WB 0.25 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Feline Leukaemia Virus p27 antibody, clone PF12J-10A recognizes feline leukaemia virus (FeLV) p27 core protein (p27).

The oncoretrovirus FeLV is responsible for both neoplastic and non-neoplastic conditions, often resulting in a poor prognosis and fatal outcome for cats persistently infected. During persistent infection the virus, soluble viral antigens and antigen containing white blood cells are continuously present in the blood and diagnosis is mainly based upon detection of the p27 antigen - using ELISA to test for p27 as it circulates free in the bloodstream, and Immunofluorescence to detect p27 within the infected white cells.

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1/100
Western Blotting 1/1,500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2551 detects a band of approximately 28kDa under reducing conditions. A strong band of 80kDa representing glycosylated FeLV gag gene product may also be present.

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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for Feline Leukaemia Virus p27 antibody

  1. Kipar, A. et al. (2000) Expression of viral proteins in feline leukemia virus-associated enteritis.
    Vet Pathol. 37 (2): 129-36.
  2. Kipar, A. et al. (2001) Comparative examination of cats with feline leukemia virus-associated enteritis and other relevant forms of feline enteritis.
    Vet Pathol. 38 (4): 359-71.
  3. Kipar, A. et al. (1998) Fatal enteritis associated with coronavirus infection in cats.
    J Comp Pathol. 119 (1): 1-14.

RRID
AB_619996
UniProt
O89811
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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