Feline Leukaemia Virus p15E antibody | PF6J-2A1

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Mouse anti Feline Leukaemia Virus p15E

Product Type
Monoclonal Antibody
Clone
PF6J-2A1
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2550 IP P WB 0.25 mg
Mouse anti Feline leukemia virus p15e antibody, clone PF6J-2A1 recognizes an epitope within the amino-terminal domain of both the 20 kDa full-length (including intracellular sequence), and the 15k Da truncated forms, of feline leukaemia virus (FeLV) p15e transmembrane envelope protein.

Persistent infection of cats with the oncoretrovirus FeLV can result in the non-neoplastic condition known as FeLV-associated enteritis (FAE). Studies have shown strong expression of the major FeLV proteins p15e and gp70 in intestinal crypt epithelial cells of cats with FAE, compared to FeLV-positive cats without intestinal alterations, thereby suggesting a pathogenic role for these envelope proteins/precursors in FAE.

Product Details

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05%Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q85525 Related reagents
Regulatory
For research purposes only

Applications of Feline Leukaemia Virus p15E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting 1/2,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2550 detects band/s of approximately 15kDa and 20kDa under reducing conditions.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Feline Leukaemia Virus p15E antibody

  1. Kipar, A. et al. (2000) Expression of viral proteins in feline leukemia virus-associated enteritis.
    Vet Pathol. 37 (2): 129-36.
  2. Kipar, A. et al. (2001) Comparative examination of cats with feline leukemia virus-associated enteritis and other relevant forms of feline enteritis.
    Vet Pathol. 38 (4): 359-71.
  3. Burns, C.C. et al. (1995) Mutations within a putative cysteine loop of the transmembrane protein of an attenuated immunodeficiency-inducing feline leukemia virus variant inhibit envelope protein processing.
    J Virol. 69 (4): 2126-32.
  4. Gwynn, S.R. et al. (2000) Feline leukemia virus envelope sequences that affect T-cell tropism and syncytium formation are not part of known receptor-binding domains.
    J Virol. 74 (13): 5754-61.
  5. Argaw, T. & Wilson,A. (2015) Mutations altering the gammaretrovirus endoproteolytic motif affect glycosylation of the envelope glycoprotein and early events of the virus life cycle.
    Virology. 475: 110-9.