Feline Leukaemia Virus p15E antibody | PF6J-2A1

Mouse anti Feline Leukaemia Virus p15E

Product Type
Monoclonal Antibody
Clone
PF6J-2A1
Isotype
IgG2a
Specificity
Feline Leukaemia Virus p15E

Product Code Applications Pack Size List Price Your Price Qty
MCA2550
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SDS Safety Datasheet SDS
IP P WB 0.25 mg loader
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loader
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Mouse anti Feline leukemia virus p15e antibody, clone PF6J-2A1 recognizes an epitope within the amino-terminal domain of both the 20 kDa full-length (including intracellular sequence), and the 15k Da truncated forms, of feline leukaemia virus (FeLV) p15e transmembrane envelope protein.

Persistent infection of cats with the oncoretrovirus FeLV can result in the non-neoplastic condition known as FeLV-associated enteritis (FAE). There is strong expression of the major FeLV proteins p15e and gp70 in intestinal crypt epithelial cells of cats with FAE, compared to FeLV-positive cats without intestinal alterations, thereby suggesting a pathogenic role for these envelope proteins/precursors in FAE (Kipar et al. 2000).

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting 1/2,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2550 detects band/s of approximately 15kDa and 20kDa under reducing conditions.

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References for Feline Leukaemia Virus p15E antibody

  1. Kipar, A. et al. (2000) Expression of viral proteins in feline leukemia virus-associated enteritis.
    Vet Pathol. 37 (2): 129-36.
  2. Kipar, A. et al. (2001) Comparative examination of cats with feline leukemia virus-associated enteritis and other relevant forms of feline enteritis.
    Vet Pathol. 38 (4): 359-71.
  3. Burns, C.C. et al. (1995) Mutations within a putative cysteine loop of the transmembrane protein of an attenuated immunodeficiency-inducing feline leukemia virus variant inhibit envelope protein processing.
    J Virol. 69 (4): 2126-32.
  4. Gwynn, S.R. et al. (2000) Feline leukemia virus envelope sequences that affect T-cell tropism and syncytium formation are not part of known receptor-binding domains.
    J Virol. 74 (13): 5754-61.
  5. Argaw, T. & Wilson,A. (2015) Mutations altering the gammaretrovirus endoproteolytic motif affect glycosylation of the envelope glycoprotein and early events of the virus life cycle.
    Virology. 475: 110-9.

RRID
AB_619995
UniProt
Q85525
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