Epstein-Barr Virus Nuclear Antigen antibody | E1-2.5
Mouse anti Epstein-Barr Virus Nuclear Antigen antibody, clone E1-2.5 (MCA2707) used for the detection of EBNA-1 in cell extracts by western blotting.
Image caption:
(a) Epitope comparison amongst the variants and those observed in the 3 cell lines studied. Red letters denote amino acid mutation. (b) Quantitation of HLA-A0201/LMP1,/LMP2A and/EBNA1 on CCRF-SB, RPMI-6666 and C666-1A2 using TCR-like mAbs based on flow cytometry. Error bars are standard deviation from an average of three independent experiments. **p < 0.01. n.d. denotes not detectable. (c) LMP1, LMP2A and EBNA1 in each of the cell lines was determined using RT-PCR and Western blot. β-actin was used as control.
From: Sim AC, Too CT, Oo MZ, Lai J, Eio MY, Song Z, Srinivasan N, Tan DA, Pang SW, Gan SU, Lee KO, Loh TK, Chen J, Chan SH, MacAry PA. Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies.
Sci Rep. 2013 Nov 18;3:3232.
Mouse anti Epstein-Barr Virus Nuclear Antigen
- Product Type
- Monoclonal Antibody
- Clone
- E1-2.5
- Isotype
- IgG2b
- Specificity
- Epstein-Barr Virus Nuclear Antigen
Antibody Quality and Performance Guarantee
| Mouse anti Epstein-Barr Virus Nuclear Antigen antibody, clone E1-2.5 recognises the repetitive Gly-Ala region of EBV (Epstein-Barr Virus) nuclear antigen 1 (EBNA-1), a viral protein expressed consistently by EBV- associated malignancies. Epstein-Barr Virus, also known as HHV-4 or Human herpesvirus 4, is a member of the herpesvirus family and one of the most common infectious viruses known, being responsible for a wide range of illnesses from infectious mononucleosis to nasal-pharyngeal cancer. EBNA-1 is the only viral protein expressed during group 1 latency and mediates the replication and partitioning of the episome during host cell division, binding to a replication origin (oriP) within the viral genome. The Gly-Ala repeat recognized by Mouse anti Epstein-Barr Virus Nuclear Antigen antibody, clone E1-2.5, inhibits the host’s CD8-restricted cytotoxic T-cell response to infected cells, by impairing antigen processing and MHC class I-restricted antigen presentation. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Epstein-Barr Virus Nuclear Antigen antibody
-
Sim, A.C. et al. (2013) Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies.
Sci Rep. 3: 3232.
Further Reading
-
Münz, C. (2004) Epstein-barr virus nuclear antigen 1: from immunologically invisible to a promising T cell target.
J Exp Med. 199 (10): 1301-4.
- Synonyms
- EBNA-1
- RRID
- AB_906013
- UniProt
- P03211
- GO Terms
- GO:0005515 protein binding
- GO:0003677 DNA binding
- GO:0006275 regulation of DNA replication
- GO:0006350 transcription
- GO:0030683 evasion by virus of host immune response
- GO:0042025 host cell nucleus
- GO:0045449 regulation of transcription
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