Thyroxine antibody | BGN/0980/322 (T4:1-32.2)
Mouse anti Thyroxine antibody, clone BGN/0980/322 (8959-9831) used for the detection of thyroxine in mouse thyroid tissue by immunofluorescence.
Image caption:
Thyroid morphology and expression of thyrocyte and C-cell markers at late embryonic stages. Staining for Nkx2-1 (A, B, F, G, K, L, P, Q), Pax8 and TG (C, H, M, R), CT and T4 (D, I, N, S), and Mash1 (E, J, O, T) at E16.5 (A–J) and E15.5 (K–T) in transverse sections from wild type and Hes1−/− mice. Boxed areas were enlarged (B–E, G–J, L–O, Q–T). Thyroid fusion was delayed by 3 days in Hes1−/− thyroids compared to wild type thyroids. tr: trachea; thy: thyroid; ma: median anlage; pt: parathyroid; UB: ultimobranchial body.
From: Carre A, Rachdi L, Tron E, Richard B, Castanet M, et al. (2011)
Hes1 Is Required for Appropriate Morphogenesis and Differentiation during Mouse Thyroid Gland Development. PLoS ONE 6(2): e16752.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Thyroxine (T4) antibody, clone BGN/0980/322 (8959-9831) used to demonstrate thyroxine expression in murine thyroid tissue sections by immunofluorescence.
Image caption:
Staining of Nkx2‐1 and T4 at E17.5 and surface area quantification demonstrating T4 retention in Tubb1−/− versus wt thyroids. The data are the percentage of T4 or calcitonin (CT) surface area versus total thyroid area (estimated from the Nkx2‐1‐stained surface area). Three mice were analysed per genotype. Scale bar: 50μm.
Data information: Results are reported as mean ± SEM. Student's t‐test, *P <0.05, **P <0.01 and ***P <0.001.
From: Stoupa A, et al.
TUBB1 mutations cause thyroid dysgenesis associated with abnormal platelet physiology.
EMBO Mol Med. 2018 Dec;10(12). pii: e9569.
doi: 10.15252/emmm.201809569.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Thyroxine
- Product Type
- Monoclonal Antibody
- Clone
- BGN/0980/322 (T4:1-32.2)
- Isotype
- IgG1
- Specificity
- Thyroxine
Antibody Quality and Performance Guarantee
| Mouse anti thyroxine antibody, clone BGN/0980/322 recognizes thyroxine, also known as T4. Thyroxine is a form of thyroid hormone secreted by the follicular cells of the thyroid gland. Mouse anti thyroxine antibody, clone BGN/0980/322 does not cross react with either rT3 or T3. |
- Target Species
- Broad
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse flounder (Paralichthys sp.) - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Thyroxine conjugated to Bovine Serum Albumin.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/20 | 1/200 |
| Immunofluorescence | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
References for Thyroxine antibody
-
Carre, A. et al. (2011) Hes1 is required for appropriate morphogenesis and differentiation during mouse thyroid gland development.
PLoS One. 6: e16752. -
Washio, Y. et al. (2015) Exposure to oxidative by-products during metamorphosis affects pigmentation patterns in flounder, Paralichthys olivaceus
Aquaculture. 435: 318-327. -
Stoupa, A. et al. (2018) TUBB1 mutations cause thyroid dysgenesis associated with abnormal platelet physiology.
EMBO Mol Med. 10 (12): pii: e9569
- Synonyms
- T4
- RRID
- AB_619268
8959-9831
0315
151581
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