hnRNP K antibody | F45 P9 C7

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Mouse anti hnRNP K

Product Type
Monoclonal Antibody
Clone
F45 P9 C7
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2622GA P* WB 0.1 mg
MCA2622 P* WB 0.2 mg
Mouse anti hnRNP K antibody, clone F45 P9 C7 specifically recognizes hnRNP K (heterogeneous ribonucleotide protein K), a 464 amino acid acidic protein found in the nucleus, cytoplasm and mitochondria, shown to be involved in transcription, mRNA shuttling and the translation and editing of RNA.

Over-expression of hnRNP K has been demonstrated in cancerous tissue including that of the colon, breast, liver and lungs, and its involvement in cell migration has been implicated in tumour metastasis. Studies have also shown that hnRNP K increases the transcriptional activity of the c-myc and c-src oncogenes.

Product Details

Target Species
Synthetic Peptide
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Rabbit
Mouse
Chicken
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Ovalbumin-conjugated synthetic peptide SVKQYSGKFF
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P61978 Related reagents
Entrez Gene
HNRNPK Related reagents
GO Terms
GO:0005515 protein binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0007165 signal transduction
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0030530 heterogeneous nuclear ribonucleoprotein complex
GO:0044419 interspecies interaction between organisms
Regulatory
For research purposes only

Applications of hnRNP K antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1/200 1/500
Western Blotting
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Colon carcinoma
Western Blotting
MCA2622 detects a band of approximately 51kDa in Calu6 and HT29 cell lysates.

Application Based External Images

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for hnRNP K antibody

  1. Carpenter, B. et al. (2006) Heterogeneous nuclear ribonucleoprotein K is over expressed, aberrantly localised and is associated with poor prognosis in colorectal cancer.
    Br J Cancer. 95 (7): 921-7.

Further Reading

  1. Bomsztyk, K. et al. (1997) Diverse molecular interactions of the hnRNP K protein.
    FEBS Lett. 403 (2): 113-5.