Histidine Tag antibody | AD1.1.10












Mouse anti Histidine Tag:Alexa Fluor® 488
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag:Alexa Fluor® 647
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag:Alk.Phos.
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag:Biotin
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag:FITC
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag:HRP
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag:DyLight®800
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
- Product Type
- Monoclonal Antibody
- Clone
- AD1.1.10
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1396A488 | F | 100 Tests/1ml |
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MCA1396A647 | F | 1 ml |
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MCA1396A | E WB | 0.1 mg |
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MCA1396B | E WB | 0.1 mg |
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MCA1396F | F IF | 0.1 mg |
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MCA1396P | E WB | 0.1 mg |
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MCA1396G | E IP WB | 2 mg |
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MCA1396GA | E IP WB | 0.1 mg |
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MCA1396 | E IP WB | 1 mg |
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MCA1396D800GA | WB | 0.1 mg |
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Product Details
- Target Species
- Synthetic Peptide
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to DyLight®800 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.01% Thiomersal
- Preservative Stabilisers
- Pack Size: 2 mg, 0.1 mg
0.09% Sodium Azide Pack Size: 1 mg0.09% Sodium Azide (NaN3) - Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
His-tag is a registered trademark of EMD Biosciences. - Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
His-tag is a registered trademark of EMD Biosciences. - Acknowledgements
- His-tag is a registered trademark of EMD Biosciences.
- Acknowledgements
- His-tag is a registered trademark of EMD Biosciences.
- Acknowledgements
- His-tag is a registered trademark of EMD Biosciences.
- Acknowledgements
- His-tag is a registered trademark of EMD Biosciences.
- Acknowledgements
- DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. His-tag is a registered trademark of EMD Biosciences.
- Regulatory
- For research purposes only
Applications of Histidine Tag antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | |||
Flow Cytometry | |||
ELISA | 1/20 | 1/1000 | |
Western Blotting | 1/1000 | 1/5000 | |
ELISA | 1/500 | 1/1000 | |
Western Blotting | 1/1000 | ||
Flow Cytometry | 1/10 | ||
Immunofluorescence | |||
ELISA | 1/20 | 1/1000 | |
Western Blotting | 1/1000 | 1/5000 | |
ELISA | |||
Immunoprecipitation | |||
Western Blotting | 1/500 | 1/1000 | |
Western Blotting | 1/1000 | 1/5000 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Secondary Antibodies Available
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
TMB Signal+ | BUF054A | E | 100 ml | ||
TMB Core | BUF056A | E | 100 ml | ||
TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for Histidine Tag antibody
-
Els Conrath, K. et al. (2001) Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs.
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Suen, J.L. et al. (2001) Characterization of self-T-cell response and antigenic determinant of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1 mice.
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Hoffmann, S.C. et al. (2007) Identification of CLEC12B, an inhibitory receptor on myeloid cells.
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Zheng, J. et al. (2007) Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen.
BMC Immunol. 8: 4. -
Bahi, A. & Dreyer, J.L. (2008) Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization.
Genes Brain Behav. 7 (2): 244-56. -
Wrighton, K.H. et al. (2009) Transforming Growth Factor {beta} Can Stimulate Smad1 Phosphorylation Independently of Bone Morphogenic Protein Receptors.
J Biol Chem. 284 (15): 9755-63. -
Diefenbacher, M. et al. (2011) The Dsl1 Tethering Complex Actively Participates in Soluble NSF (N-Ethylmaleimide-sensitive Factor) Attachment Protein Receptor (SNARE) Complex Assembly at the Endoplasmic Reticulum in Saccharomyces cerevisiae.
J Biol Chem. 286: 25027-38. -
Alvarez, M.M. et al. (2010) Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method.
PLoS One. 5: e10176. -
Bahi, A. et al. (2008) The role of tissue-type plasminogen activator system in amphetamine-induced conditional place preference extinction and reinstatement.
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Gunnarsen, K.S. et al. (2010) Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA.
BMC Biotechnol. 10: 8. -
Hwang, H.Y. et al. (2008) Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable fragment.
Mol Immunol. 45: 2570-80. -
De Vooght, L. et al. (2012) Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly.
Microb Cell Fact. 11: 23. -
Saerens, D. et al. (2004) Single domain antibodies derived from dromedary lymph node and peripheral blood lymphocytes sensing conformational variants of prostate-specific antigen.
J Biol Chem. 279 (50): 51965-72. -
Than, N.G. et al. (2014) Evolutionary origins of the placental expression of chromosome 19 cluster galectins and their complex dysregulation in preeclampsia.
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Elders RC et al. (2014) Recombinant canine IgE Fc and an IgE Fc-TRAIL fusion protein bind to neoplastic canine mast cells.
Vet Immunol Immunopathol. 159 (1-2): 29-40. -
Chin, S.E. et al. (2015) Isolation of high-affinity, neutralizing anti-idiotype antibodies by phage and ribosome display for application in immunogenicity and pharmacokinetic analyses.
J Immunol Methods. 416: 49-58. -
Peyrassol, X. et al. (2016) Development by Genetic Immunization of Monovalent Antibodies (Nanobodies) Behaving as Antagonists of the Human ChemR23 Receptor.
J Immunol. 196 (6): 2893-901. -
Kim H & Loparo JJ (2016) Multistep assembly of DNA condensation clusters by SMC.
Nat Commun. 7: 10200. -
Borg M et al. (2014) A novel interaction between Rab7b and actomyosin reveals a dual role in intracellular transport and cell migration.
J Cell Sci. 127 (Pt 22): 4927-39. -
De Meyer, T. et al. (2015) Comparison of VHH-Fc antibody production in Arabidopsis thaliana, Nicotiana benthamiana and Pichia pastoris.
Plant Biotechnol J. 13 (7): 938-47. -
Siddiqui AA et al. (2015) Humoral immune responses to a recombinant Plasmodium vivax tryptophan-rich antigen among Plasmodium vivax-infected patients and its localization in the parasite.
Appl Biochem Biotechnol. 175 (4): 2166-77. -
Warnecke, A. et al. (2017) Nitration of MOG diminishes its encephalitogenicity depending on MHC haplotype.
J Neuroimmunol. 303: 1-12. -
Bertucci, A. et al. (2011) A new coral carbonic anhydrase in Stylophora pistillata.
Mar Biotechnol (NY). 13 (5): 992-1002. -
Liu, C.C. et al. (2016) The Fimbrial Protein is a Virulence Factor and Potential Vaccine Antigen of Avibacterium paragallinarum.
Avian Dis. 60 (3): 649-55. -
Boujon, C.L. et al. (2017) Development and validation of an immunohistochemistry procedure for the detection of a neurotropic bovine astrovirus.
J Virol Methods. 239: 26-33. -
Cartwright, S.P. et al. (2017) Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris.
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Thanongsaksrikul, J. et al. (2018) Identification and production of mouse scFv to specific epitope of enterovirus-71 virion protein-2 (VP2).
Arch Virol. 163 (5): 1141-1152. -
Gunnarsen, K.S. et al. (2018) Soluble T-cell receptor design influences functional yield in an E. coli chaperone-assisted expression system.
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