Histidine Tag antibody | AD1.1.10
Horseradish peroxidase conjugated Mouse anti Histidine tag antibody clone AD1.1.0 (MCA1396P) used for the detection of recombinant scTCR varients by western blotting.
Image caption:
4B2A1 scTCR and rFkpA expression level. Western blot showing periplasmic expression levels of 4B2A1 scTCR variants and rFkpA from pHOG, pFKPEN (pEN) and pFKPEI (pEI) vectors under different expression conditions, detected with anti-His tag -HRP Ab and anti-Flag- M2 HRP Ab as described. The scTCR and rFkpA were developed with SuperSignal West Pico substrate and exposed to BioMax MR film for for 30 min and 30 sec, respectively, for the upper and lower panels. The expression analysis was repeated twice, and representative results are shown.
From: Gunnarsen KS, Lunde E, Kristiansen PE, Bogen B, Sandlie I, Løset GÅ.
Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA.
BMC Biotechnol. 2010 Feb 3;10:8.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Horseradish peroxidase conjugated Mouse anti Histidine tag antibody clone AD1.1.0 (MCA1396P) used for the detection of recombinant scTCR varients by western blotting.
Image caption:
7A10B2 scTCR and rFkpA expression level. Western blot showing periplasmic expression levels of 7A10B2 scTCR variants and rFkpA from pHOG, pFKPEN (pEN) and pFKPEI (pEI) vectors under different expression conditions, detected with anti-His tag-HRP and anti-Flag-M2-HRP as described. The scTCR and rFkpA were developed with SuperSignal West Pico substrate and exposed to BioMax MR film for for 30 min and 30 sec, respectively, for the upper and lower panels. The expression analysis was repeated twice, and representative results are shown.
From: Gunnarsen KS, Lunde E, Kristiansen PE, Bogen B, Sandlie I, Løset GÅ.
Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA.
BMC Biotechnol. 2010 Feb 3;10:8.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Horseradish peroxidase conjugated Mouse anti Histidine tag antibody clone AD1.1.0 (MCA1396P) used for the detection of recombinant scTCR varients by western blotting.
Image caption:
Purity of proteins. (A) and (B); S75 size exclusion chromatography of scTCR 4B2A1 Vαβ and Vβα respectively. 50 μl of the IMAC purified material was subject to the purification procedure. In (A) fractions A14-B1 corresponds to the higher aggregates, while fraction B5-B7 corresponds to the monomeric scTCR 4B2A1 Vαβ, the monomeric fraction is highlighted with an arrow. In (B) fraction A15-B1 and B3-B4 corresponds to the higher aggregates, while fraction B6-B7 corresponds to the monomeric scTCR 4B2A1 Vβα, the monomeric fraction is highlighted with an arrow. (C) and (D); Western blots showing reduced and non-reduced scTCR Vαβ and Vβα after IMAC purification and S75 size exclusion chromatography. Samples of 0.25 μg were loaded on the gel and analysed with anti-His tag-HRP.
From: Gunnarsen KS, Lunde E, Kristiansen PE, Bogen B, Sandlie I, Løset GÅ.
Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA.
BMC Biotechnol. 2010 Feb 3;10:8.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Horseradishperoxidase conjugated Mouse anti Histidine tag antibody, clone AD1.1.10 (MCA1396P) used for the detection of HIS tagged protein constructs by western blotting.
Image caption:
Effect of host strain and induction conditions on periplasmic TCR expression.
(A) Representative western blots showing the expression profile of the TCR formats in either E. coli XL1-Blue (X) or RosettaBlue™ (R) where IPTG induction of protein expression either was omitted (-) or included (+). Normalized expression cultures were fractionated before the presence of TCR in the periplasmic fractions was analyzed by western blot detection using an anti-His-HRP antibody (n = 3). (B) The bacterial growth characteristics were monitored by measuring the OD600nm of E. coli XL1-blue (X) and RosettaBlue™ (R) bacterial cultures after overnight protein expression without (-) or with (+) IPTG induction at 30°C. n = 3 for all constructs except for controls (n = 4) and the dsTCR constructs (n = 1). Standard deviations are indicated where possible. (A, B) Cells expressing scFv anti-phOx were used as positive control and untransformed cells as negative control.
From: Gunnarsen KS, Høydahl LS, Neumann RS, Bjerregaard-Andersen K, Nilssen NR, Sollid LM, et al. (2018)
Soluble T-cell receptor design influences functional yield in an E. coli chaperone-assisted expression system.
PLoS ONE 13(4): e0195868.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Horseradishperoxidase conjugated Mouse anti Histidine tag antibody, clone AD1.1.10 (MCA1396P) used for the detection of HIS tagged protein constructs by western blotting.
Image caption:
Expression characteristics of the recombinant TCR formats.
Representative western blot showing the expression profile of the TCR formats. E. coli RosettaBlue™ expression cultures were normalized and fractionated into; M, medium; P, periplasmic; C, cytosolic fractions before analysis by western blots (n = 3–4). All samples were detected with anti-His-HRP antibody. Expression of scFv anti-phOx was included as positive control.
From: Gunnarsen KS, Høydahl LS, Neumann RS, Bjerregaard-Andersen K, Nilssen NR, Sollid LM, et al. (2018)
Soluble T-cell receptor design influences functional yield in an E. coli chaperone-assisted expression system.
PLoS ONE 13(4): e0195868.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Histidine Tag antibody, clone AD1.1.10 (MCA1396) used to detect LAG3 specific, HIS tagged antibody by western blotting.
Image caption:
Characterization of the anti-LAG3 scFvF7.
D.Binding analysis of scFvF7. 0.5 μg of both recombinant LAG3 and glucose oxidase (GO) proteins were loaded in each replicate well on a 12% SDS-PAGE under reducing condition and transferred to filter paper. Strips from the filter were then incubated with the indicted primary antibodies. Anti-6 his mAb was used as a positive control for LAG3 recombinant protein (which has a 6-histidines tag at its C terminal end). As additional negative controls, two strips were incubated respectively with the anti-Flag antibody and the HRP-conjugated anti-mouse antibody, in order to monitor eventual non-specific signals due to the secondary antibodies used to detect scFv antibodies. The ELISA shown below (performed with the same secondary Abs) is a check for the reactivity of the scFv-containing supernatants (scFvGO and scFvF7) used in the assay. Arrows indicate relevant signals. Molecular markers in kilodaltons (kDa) are reported on the right. A representative of three independent assays is shown.
From: Ascione A, Arenaccio C, Mallano A, Flego M, Gellini M, Andreotti M, Fenwick C, Pantaleo G, Vella S, Federico M.
Development of a novel human phage display-derived anti-LAG3 scFv antibody targeting CD8+ T lymphocyte exhaustion.
BMC Biotechnol. 2019 Oct 17;19(1):67.
doi: 10.1186/s12896-019-0559-x.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Histidine Tag antibody, clone AD1.1.10 (MCA1396) used to detect LAG3 specific, HIS tagged antibody by western blotting.
Image caption:
Construction and characterization of the divalent scFvF7-Fc Ab.
Anti 6 × histidine western blot analysis carried on supernatants of CHO cells transfected with either the scFvF7-Fc expressing vector (lane 1), or transfected with the empty vector (lane 2). Molecular weight markers (Mk) are reported on the left. The results are representative of four independent assays.
From: Ascione A, Arenaccio C, Mallano A, Flego M, Gellini M, Andreotti M, Fenwick C, Pantaleo G, Vella S, Federico M.
Development of a novel human phage display-derived anti-LAG3 scFv antibody targeting CD8+ T lymphocyte exhaustion.
BMC Biotechnol. 2019 Oct 17;19(1):67.
doi: 10.1186/s12896-019-0559-x.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Histidine tag antibody, clone AD1.1.10 (MCA1396) used to detect Histidine tagged spiHIFα by western blotting.
Image caption:
HEK293 cells were transfected with 0.1μg of spiHIFα or spiHIFβ plasmids. 24h post-transfection, cells were maintained in normoxic (21%) or hypoxic (<1%) conditions during 6h. Whole cell extracts migrated within a 7.5% SDS PAGE gel, were analyzed by immuno blot with different antibodies: anti-Histidine tag for SpiHIFα, anti-Myc for SpiHIFβ, and anti-actin as a loading control.
From: Zoccola D, Morain J, Pagès G, Caminiti-Segonds N, Giuliano S, Tambutté S, et al. (2017)
Structural and functional analysis of coral Hypoxia Inducible Factor.
PLoS ONE 12(11): e0186262.
doi: 10.1371/journal.pone.0186262.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Histidine Tag:Alexa Fluor® 488
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag:Alexa Fluor® 647
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag:Alk.Phos.
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag:Biotin
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag:FITC
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag:HRP
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag:DyLight®800
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
Mouse anti Histidine Tag:DyLight®550
Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.
- Product Type
- Monoclonal Antibody
- Clone
- AD1.1.10
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA1396A488 | F | 100 Tests/1ml |
|
|
| MCA1396A647 | F | 1 ml |
|
|
| MCA1396A | E WB | 0.1 mg |
|
|
| MCA1396B | E WB | 0.1 mg |
|
|
| MCA1396F | F IF | 0.1 mg |
|
|
| MCA1396P | E WB | 0.1 mg |
|
|
| MCA1396G | E IP WB | 2 mg |
|
|
| MCA1396GA | E IP WB | 0.1 mg |
|
|
| MCA1396 | E IP WB | 1 mg |
|
|
| MCA1396D800GA | WB | 0.1 mg |
|
|
| MCA1396D550 | F IF WB | 0.1 mg |
|
Mouse anti Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian (Hoffmann et al. 2007) and Hwang et al. 2008) and non-mammalian (Zheng et al. 2007; Gunnarsen et al. 2010; and de Vooght et al. 2012) cell lines.
In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
Product Details
- Target Species
- Synthetic Peptide
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to DyLight®800 - liquid
- Product Form
- Purified IgG conjugated to DyLight®550 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.01% Thiomersal
- Preservative Stabilisers
- Pack Size: 2 mg, 0.1 mg
0.09% Sodium Azide Pack Size: 1 mg0.09% Sodium Azide (NaN3) - Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- PAX6 transcription factor linked to histidine tag.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
His-tag is a registered trademark of EMD Biosciences. - Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
His-tag is a registered trademark of EMD Biosciences. - Acknowledgements
- His-tag is a registered trademark of EMD Biosciences.
- Acknowledgements
- His-tag is a registered trademark of EMD Biosciences.
- Acknowledgements
- His-tag is a registered trademark of EMD Biosciences.
- Acknowledgements
- His-tag is a registered trademark of EMD Biosciences.
- Acknowledgements
- DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. His-tag is a registered trademark of EMD Biosciences.
- Acknowledgements
- DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. His-tag is a registered trademark of EMD Biosciences.
- Regulatory
- For research purposes only
Applications of Histidine Tag antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Flow Cytometry | |||
| ELISA | 1/20 | 1/1000 | |
| Western Blotting | 1/1000 | 1/5000 | |
| ELISA | 1/500 | 1/1000 | |
| Western Blotting | 1/1000 | ||
| Flow Cytometry | 1/10 | ||
| Immunofluorescence | |||
| ELISA | 1/20 | 1/1000 | |
| Western Blotting | 1/1000 | 1/5000 | |
| ELISA | |||
| Immunoprecipitation | |||
| Western Blotting | 1/500 | 1/1000 | |
| Western Blotting | 1/1000 | 1/5000 | |
| Flow Cytometry | |||
| Immunofluorescence | 1/50 | 1/200 | |
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for Histidine Tag antibody
-
Els Conrath, K. et al. (2001) Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs.
J Biol Chem. 276 (10): 7346-50. -
Suen, J.L. et al. (2001) Characterization of self-T-cell response and antigenic determinant of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1 mice.
Immunol. 103: 301-309. -
Hoffmann, S.C. et al. (2007) Identification of CLEC12B, an inhibitory receptor on myeloid cells.
J Biol Chem. 282 (31): 22370-5. -
Zheng, J. et al. (2007) Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen.
BMC Immunol. 8: 4. -
Bahi, A. & Dreyer, J.L. (2008) Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization.
Genes Brain Behav. 7 (2): 244-56. -
Wrighton, K.H. et al. (2009) Transforming Growth Factor {beta} Can Stimulate Smad1 Phosphorylation Independently of Bone Morphogenic Protein Receptors.
J Biol Chem. 284 (15): 9755-63. -
Diefenbacher, M. et al. (2011) The Dsl1 Tethering Complex Actively Participates in Soluble NSF (N-Ethylmaleimide-sensitive Factor) Attachment Protein Receptor (SNARE) Complex Assembly at the Endoplasmic Reticulum in Saccharomyces cerevisiae.
J Biol Chem. 286: 25027-38. -
Alvarez, M.M. et al. (2010) Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method.
PLoS One. 5: e10176. -
Bahi, A. et al. (2008) The role of tissue-type plasminogen activator system in amphetamine-induced conditional place preference extinction and reinstatement.
Neuropsychopharmacology. 33: 2726-34. -
Gunnarsen, K.S. et al. (2010) Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA.
BMC Biotechnol. 10: 8. -
Hwang, H.Y. et al. (2008) Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable fragment.
Mol Immunol. 45: 2570-80. -
De Vooght, L. et al. (2012) Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly.
Microb Cell Fact. 11: 23. -
Saerens, D. et al. (2004) Single domain antibodies derived from dromedary lymph node and peripheral blood lymphocytes sensing conformational variants of prostate-specific antigen.
J Biol Chem. 279 (50): 51965-72. -
Than, N.G. et al. (2014) Evolutionary origins of the placental expression of chromosome 19 cluster galectins and their complex dysregulation in preeclampsia.
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Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
