CD8 antibody | 38.65

Product Code Applications Pack Size List Price Your Price Qty
MCA2216GA
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SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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loader
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Mouse anti Sheep CD8 antibody, clone 38.65 recognizes the ovine CD8 cell surface antigen, which is expressed by the cytotoxic/suppressor subset of T lymphocytes.

Under reducing conditions, the antigens immunoprecipitated by Mouse anti Sheep CD8 antibody, clone 38.65 migrate at ~33 kDa and ~36 kDa.

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Ovine efferent lymphocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD8 antibody

  1. Maddox, J.F. et al. (1985) Surface antigens, SBU-T4 and SBU-T8, of sheep T lymphocyte subsets defined by monoclonal antibodies.
    Immunology. 55 (4): 739-48.
  2. Mackay, C.R. et al. (1986) Three distinct subpopulations of sheep T lymphocytes.
    Eur J Immunol. 16 (1): 19-25.
  3. Mackay, C.R. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.
    Cell Immunol. 110 (1): 46-55.
  4. Mackay, C.R. et al. (1989) Gamma/delta T cells express a unique surface molecule appearing late during thymic development.
    Eur J Immunol. 19 (8): 1477-83.
  5. Mackay, C.R. et al. (1986) Thymocyte subpopulations during early fetal development in sheep.
    J Immunol. 136 (5): 1592-9.
  6. Breugelmans, S. et al. (2010) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
    Acta Histochem. 113(4):416-22
  7. Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
    Clin Vaccine Immunol. 16 (7): 1003-11.
  8. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
    Immunology. 107: 366-72.
  9. View The Latest Product References
  10. Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.
    Vet Res. 41: 4.
  11. Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
    Clin Vaccine Immunol. 16: 1003-11.
  12. Kallapur, S.G. et al. (2011) Pulmonary and Systemic Inflammatory Responses to Intraamniotic IL-1 alpha in fetal sheep.
    Am J Physiol Lung Cell Mol Physiol. 301(3):L285-95
  13. Bruce, C.J. et al. (1999) Depletion of bovine CD8+ T cells with chCC63, a chimaeric mouse-bovine antibody.
    Vet Immunol Immunopathol. 71 (3-4): 215-31.
  14. Nfon, C.K.et al (2012) Innate Immune Response to Rift Valley Fever Virus in Goats.
    PLoS Negl Trop Dis.6 (4): e1623.
  15. Lybeck, K.R. et al. (2012) Intestinal Strictures, Fibrous Adhesions and High Local Interleukin-10 Levels in Goats Infected Naturally with Mycobacterium avium subsp. paratuberculosis.
    J Comp Pathol. 148: 157-72.
  16. Olsen, L. et al. (2015) The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1(+) NK cells and by NCR1(-) CD8(+) cell recruitment.
    Vet Res. 46: 28.
  17. Goh, S. et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
    PLoS One. 11 (9): e0162571.
  18. Arranz-Solís, D. et al. (2016) Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
    Vet Res. 47: 2.
  19. Baliu-piqué, M. et al. (2019) Age-related distribution and dynamics of T-cells in blood and lymphoid tissues of goats.
    Dev Comp Immunol. 93: 1-10.
  20. Schwarz, E.R. et al. (2020) Experimental Infection of Mid-Gestation Pregnant Female and Intact Male Sheep with Zika Virus.
    Viruses. 12 (3)Mar 07 [Epub ahead of print].
  21. Zhang, H. et al. (2020) Thiamine ameliorates inflammation of the ruminal epithelium of Saanen goats suffering from subacute ruminal acidosis.
    J Dairy Sci. 103 (2): 1931-43.
  22. Ramos, A. et al. (2018) Melatonin enhances responsiveness to Dichelobacter nodosus vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes.
    Vet Immunol Immunopathol. 206: 1-8.
  23. Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis. REV1 vaccine produced in bioreactor.
    Vet Immunol Immunopathol. 198: 44-53.
  24. Ducournau, C. et al. (2020) Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep.
    Front Immunol. 11: 2183.
  25. Wooldridge, A.L. et al. (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: potential mechanisms for programming asthma and allergy.
    J Physiol. 597 (16): 4251-62.
  26. Yang, J. et al. (2022) Baseline T-lymphocyte and cytokine indices in sheep peripheral blood.
    BMC Vet Res. 18 (1): 165.

Flow Cytometry

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