CD8 antibody | 38.65
Mouse anti Sheep CD8 antibody, clone 38.65 used for the detection of CD8 expressing lymphocytes in ovine Peyer's patch and jejenum by flow cytometry and immunofluorescence.
Image caption:
CD8+ lymphocyte increase in the jejunum and jejunal Peyer's patches. (A) Cells isolated from jejunal Peyer's patches (JPP) and jejunum were double labelled with anti CD8 and NCR1 mAbs and analyzed by flow cytometry. The plots show data from a 6 dpi inoculated lamb and its 7 day-old matched control and are representative of 8 matched pairs of 7 day-old lambs. The gating used for the analyses shown in B is indicated with the corresponding colors. (B) The percentages of the 3 different populations of CD8+ lymphocytes corresponding to the 3 gates shown in (A) were analyzed in jejunum and JPP from lambs 2–6 dpi. Control lambs (open symbols), inoculated lambs (filled symbols). The means are indicated in red. The comparisons were made with the Mann–Whitney (control/inoculated) and Wilcoxon tests (paired data). Statistically significant differences are indicated with * p < 0.05, ** p < 0.01, *** p < 0.001. (C-D) Section of an ileal Peyer's patch (IPP) from a 7 day old control (C) and a 6 dpi lamb (D) were double labelled with anti CD8 (green) and Ki-67 (blue) antibodies. The images shown are obtained by merging these images with the white light image. The double positive cells (arrow) display CD8 cytoplasmic staining and Ki67 nuclear staining. Pinpoint sized spots (arrowhead) are identified as autofluorescence. Dome (d), lamina propria (lp). Bar 50 μm.
From: Olsen et al. "The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1− CD8+ cell recruitment".
Vet Res 2015 46:28.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep CD8 antibody, clone 38.65 used for the detection of CD8 expressing lymphocytes in ovine Peyer's patch and jejenum by immunofluorescence.
Image caption:
Significant increase of CD8+ cells in the ileum of infected lambs. Ileal Peyer's patches sections from a pair of 6 day-old matched lambs, control (A) and inoculated (B), were labelled with Ab against CD3 (red), CD8 (green) and γδTCR (blue). Increased numbers of yellow CD3+/CD8+ cells (arrow) in the lamina propria (lp) and dome (d) were seen in the inoculated animals compared with the controls. Few or no CD3+/CD8+/γδTCR+ cells were observed. Light blue dots (arrowhead) were identified as autofluorescence and thus differentiated from the specific labelling which was localised in the cell membrane. Absorptive epithelium (ae), follicle-associated epithelium (fae), follicle (f), interfollicular area (ifa). Bar 50 μm. (C) The increase of CD3+/CD8+ cells in the ileal Peyer's patch was demonstrated by quantitative analysis of positive cells per mm2 in villi and dome, including the covering epithelium. The graphs show the median with 95% confidence interval constructed using the Bernoulli-Wilcoxon procedure. Statistically significant differences are indicated as ** p < 0.01 and *** p < 0.001.
From: Olsen et al.
The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1− CD8+ cell recruitment.
Veterinary Research 2015 46:28.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep CD8 antibody, clone 38.65 used for the detection of CD8 expressing lymphocytes in ovine jejenum by flow cytometry.
Image caption:
Perforin+ lymphocytes among CD8+ lymphocytes in the gut.(A) Cells extracted from gut tissues were double labelled with anti NCR1 and CD8 mAbs then fixed and permeabilized and labelled with an anti perforin mAb. The plot shows (example of jejunum at 6 dpi) the gating of the lymphocyte populations analyzed. (B) Lymphocytes from an inoculated lamb (3 dpi) and its control were gated either on the CD8+/NCR1+ or the CD8tot/NCR1- populations then analyzed for the perforin content. The red line limited histogram corresponds to the inoculated lamb (In), the black line limited histogram to its matched control (Co) and the gray filled histogram to the isotype control of the perforin mAb (iso) and the mean fluorescence intensity (MFI) is indicated for each histogram. (C) The analysis of the perforin expression by CD8+ lymphocyte sub-populations (gates shown on Figure 7A) was performed in 3 control and 4 age-matched 6 dpi inoculated lambs. The comparisons were made with the Mann–Whitney (control/inoculated) and Wilcoxon tests (paired data). Statistically significant differences are indicated with ** p < 0.01 and *** p < 0.001.
From: Olsen et al. "The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1− CD8+ cell recruitment".
Vet Res 2015 46:28.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep CD8 antibody, clone 38.65 used for the detection of CD8 expressing lymphocytes in ovine jejenum by flow cytometry.
Image caption:
Expression of CD25 on small intestinal NCR1+ and CD8+ lymphocytes. (A) The expression of the activation marker CD25 was analyzed at 6 dpi in the populations gated as indicated. The individual percentages (B) and the mean fluorescence intensity (MFI) of CD25+ cells (C) are shown for the 3 populations. Medians are shown with red bars. Control lambs (open symbols), inoculated lambs (filled symbols). In C, the results of the two experiments are shown with red or black symbols. The comparisons were made with the Mann–Whitney (control/inoculated) and Wilcoxon tests (paired data). Statistically significant differences are indicated with ** p < 0.01 and *** p < 0.001.
From: Olsen et al. "The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1− CD8+ cell recruitment".
Veterinary Research 2015 46:28.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD8 antibody, clone 38.65 (MCA2216GA) used to demonstrate the presence of CD8 expressing T cells in ovine placenta during gestation by immunohistochemistry on cryostat sections.
Image caption:
Comparison of the immunohistochemical labeling of T inflammatory cells and parasite antigen in the placenta. This panel compares the distribution and frequency of T lymphocytes (CD3, CD4 and CD8 antigens) and parasite antigen immunohistochemically labeled in samples from the three groups. Pictures showing the labeling of T cells (first three rows) were taken at 75× and those of the parasite antigen (last row) were taken at 150×. F: foetal mesenchyme, M: maternal endometrial stalk.
From: Arranz-Solís D, Benavides J, Regidor-Cerrillo J, Horcajo P, Castaño P, Del Carmen Ferreras M, Jiménez-Pelayo L, Collantes-Fernández E, Ferre I, Hemphill A, Pérez V, Ortega-Mora LM.
Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
Vet Res. 2016 Jan 6;47(1):2.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep CD8 antibody, clone 38.65 recognizes the ovine CD8 cell surface antigen, which is expressed by the cytotoxic/suppressor subset of T lymphocytes.
Under reducing conditions, the antigens immunoprecipitated by Mouse anti Sheep CD8 antibody, clone 38.65 migrate at ~33 kDa and ~36 kDa.
Under reducing conditions, the antigens immunoprecipitated by Mouse anti Sheep CD8 antibody, clone 38.65 migrate at ~33 kDa and ~36 kDa.
Product Details
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Ovine efferent lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD8 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA929F | F | 100 Tests |
|
|
| Mouse IgG2a Negative Control:RPE | MCA929PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Sheep CD4:Alexa Fluor® 647 | MCA2213A647 | F | 100 Tests/1ml |
|
|
| Mouse anti Sheep CD4:FITC | MCA2213F | F | 0.1 mg |
|
|
| Mouse anti Sheep CD4:RPE | MCA2213PE | F | 100 Tests |
|
Product Specific References
References for CD8 antibody
-
Maddox, J.F. et al. (1985) Surface antigens, SBU-T4 and SBU-T8, of sheep T lymphocyte subsets defined by monoclonal antibodies.
Immunology. 55 (4): 739-48. -
Mackay, C.R. et al. (1986) Three distinct subpopulations of sheep T lymphocytes.
Eur J Immunol. 16 (1): 19-25. -
Mackay, C.R. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.
Cell Immunol. 110 (1): 46-55. -
Mackay, C.R. et al. (1989) Gamma/delta T cells express a unique surface molecule appearing late during thymic development.
Eur J Immunol. 19 (8): 1477-83. -
Mackay, C.R. et al. (1986) Thymocyte subpopulations during early fetal development in sheep.
J Immunol. 136 (5): 1592-9. -
Breugelmans, S. et al. (2010) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
Acta Histochem. 113(4):416-22 -
Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
Clin Vaccine Immunol. 16 (7): 1003-11. -
Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
Immunology. 107: 366-72. -
Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.
Vet Res. 41: 4. -
Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
Clin Vaccine Immunol. 16: 1003-11. -
Kallapur, S.G. et al. (2011) Pulmonary and Systemic Inflammatory Responses to Intraamniotic IL-1 alpha in fetal sheep.
Am J Physiol Lung Cell Mol Physiol. 301(3):L285-95 -
Bruce, C.J. et al. (1999) Depletion of bovine CD8+ T cells with chCC63, a chimaeric mouse-bovine antibody.
Vet Immunol Immunopathol. 71 (3-4): 215-31. -
Nfon, C.K.et al (2012) Innate Immune Response to Rift Valley Fever Virus in Goats.
PLoS Negl Trop Dis.6 (4): e1623. -
Lybeck, K.R. et al. (2012) Intestinal Strictures, Fibrous Adhesions and High Local Interleukin-10 Levels in Goats Infected Naturally with Mycobacterium avium subsp. paratuberculosis.
J Comp Pathol. 148: 157-72. -
Olsen, L. et al. (2015) The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1(+) NK cells and by NCR1(-) CD8(+) cell recruitment.
Vet Res. 46: 28. -
Goh, S. et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
PLoS One. 11 (9): e0162571. -
Arranz-Solís, D. et al. (2016) Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
Vet Res. 47: 2. -
Baliu-piqué, M. et al. (2019) Age-related distribution and dynamics of T-cells in blood and lymphoid tissues of goats.
Dev Comp Immunol. 93: 1-10. -
Schwarz, E.R. et al. (2020) Experimental Infection of Mid-Gestation Pregnant Female and Intact Male Sheep with Zika Virus.
Viruses. 12 (3)Mar 07 [Epub ahead of print]. -
Zhang, H. et al. (2020) Thiamine ameliorates inflammation of the ruminal epithelium of Saanen goats suffering from subacute ruminal acidosis.
J Dairy Sci. 103 (2): 1931-43.
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