CD8 antibody | 38.65









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F IF C ResetMouse anti Sheep CD8
- Product Type
- Monoclonal Antibody
- Clone
- 38.65
- Isotype
- IgG2a
- Specificity
- CD8
Mouse anti Sheep CD8 antibody, clone 38.65 recognizes the ovine CD8 cell surface antigen, which is expressed by the cytotoxic/suppressor subset of T lymphocytes. Under reducing conditions, the antigens immunoprecipitated by Mouse anti Sheep CD8 antibody, clone 38.65 migrate at ~33 kDa and ~36 kDa. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Ovine efferent lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
1/50 | 1/100 |
Immunohistology - Frozen | ![]() |
||
Immunoprecipitation | ![]() |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse anti Sheep CD4:RPE | MCA2213PE | F | 100 Tests |
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Description | Mouse anti Sheep CD4:RPE | ||||||
Mouse anti Sheep CD4:Alexa Fluor® 647 | MCA2213A647 | F | 100 Tests/1ml |
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Description | Mouse anti Sheep CD4:Alexa Fluor® 647 | ||||||
Mouse anti Sheep CD4:FITC | MCA2213F | F | 0.1 mg |
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Description | Mouse anti Sheep CD4:FITC |
References for CD8 antibody
-
Maddox, J.F. et al. (1985) Surface antigens, SBU-T4 and SBU-T8, of sheep T lymphocyte subsets defined by monoclonal antibodies.
Immunology. 55 (4): 739-48. -
Mackay, C.R. et al. (1986) Three distinct subpopulations of sheep T lymphocytes.
Eur J Immunol. 16 (1): 19-25. -
Mackay, C.R. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.
Cell Immunol. 110 (1): 46-55. -
Mackay, C.R. et al. (1989) Gamma/delta T cells express a unique surface molecule appearing late during thymic development.
Eur J Immunol. 19 (8): 1477-83. -
Mackay, C.R. et al. (1986) Thymocyte subpopulations during early fetal development in sheep.
J Immunol. 136 (5): 1592-9. -
Breugelmans, S. et al. (2010) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
Acta Histochem. 113(4):416-22 -
Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
Clin Vaccine Immunol. 16 (7): 1003-11. -
Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
Immunology. 107: 366-72.
View The Latest Product References
-
Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.
Vet Res. 41: 4. -
Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
Clin Vaccine Immunol. 16: 1003-11. -
Kallapur, S.G. et al. (2011) Pulmonary and Systemic Inflammatory Responses to Intraamniotic IL-1 alpha in fetal sheep.
Am J Physiol Lung Cell Mol Physiol. 301(3):L285-95 -
Bruce, C.J. et al. (1999) Depletion of bovine CD8+ T cells with chCC63, a chimaeric mouse-bovine antibody.
Vet Immunol Immunopathol. 71 (3-4): 215-31. -
Nfon, C.K.et al (2012) Innate Immune Response to Rift Valley Fever Virus in Goats.
PLoS Negl Trop Dis.6 (4): e1623. -
Lybeck, K.R. et al. (2012) Intestinal Strictures, Fibrous Adhesions and High Local Interleukin-10 Levels in Goats Infected Naturally with Mycobacterium avium subsp. paratuberculosis.
J Comp Pathol. 148: 157-72. -
Olsen, L. et al. (2015) The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1(+) NK cells and by NCR1(-) CD8(+) cell recruitment.
Vet Res. 46: 28. -
Goh, S. et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
PLoS One. 11 (9): e0162571. -
Arranz-Solís, D. et al. (2016) Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
Vet Res. 47: 2. -
Baliu-piqué, M. et al. (2019) Age-related distribution and dynamics of T-cells in blood and lymphoid tissues of goats.
Dev Comp Immunol. 93: 1-10. -
Schwarz, E.R. et al. (2020) Experimental Infection of Mid-Gestation Pregnant Female and Intact Male Sheep with Zika Virus.
Viruses. 12 (3)Mar 07 [Epub ahead of print]. -
Zhang, H. et al. (2020) Thiamine ameliorates inflammation of the ruminal epithelium of Saanen goats suffering from subacute ruminal acidosis.
J Dairy Sci. 103 (2): 1931-43. -
Ramos, A. et al. (2018) Melatonin enhances responsiveness to Dichelobacter nodosus vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes.
Vet Immunol Immunopathol. 206: 1-8. -
Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis. REV1 vaccine produced in bioreactor.
Vet Immunol Immunopathol. 198: 44-53. -
Ducournau, C. et al. (2020) Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep.
Front Immunol. 11: 2183. -
Wooldridge, A.L. et al. (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: potential mechanisms for programming asthma and allergy.
J Physiol. 597 (16): 4251-62. -
Yang, J. et al. (2022) Baseline T-lymphocyte and cytokine indices in sheep peripheral blood.
BMC Vet Res. 18 (1): 165.
MCA2216GA


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