CD335 antibody | EC1.1

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Mouse anti Sheep CD335

Product Type
Monoclonal Antibody
Clone
EC1.1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5933GA C F P* 0.1 mg
Mouse anti Sheep CD335, clone EC1.1, recognizes the ovine homologue of human CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1.

CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which function as an activating receptor.

Research has indicated that, in mammals, the CD335 antigen is confined to NK cells (Westergard et. al. 2004) and that it is primarily expressed by a subset of natural killer (NK) cells which are CD3-ve, CD4-ve and CD2+ve with a small population also being CD8+ve (Storset et. al. 2004). No expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.

NK cells are cytotoxic and form a key component of the innate immune system where they are involved in mediating immunity to tumours and pathogens and in particular are critical to the process where the host recognizes and rejects tumour and viral infected cells.

Clone EC1.1 (originally named as Gr13.1) is the first antibody specifically raised to ovine CD335 and provides a reagent enabling researchers to identify ovine NKp46 positive NK cells and may facilitate an increased understanding of the ovine immune system and in particular its role in host pathogen defence (Connelley et. al. 2011).

In addition to clone EC1.1, clones AKS1, which recognises CD335 (NKp46) in bovine and other ruminants, and VIV-KM1, which recognises porcine CD335, are also available from Bio-Rad.

Product Details

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
OvNKp46-mFcγ2b fusion protein
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the X63 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch

More Information

UniProt
E5LDC2 Related reagents
Q863H2 Related reagents
Entrez Gene
NCR1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004872 receptor activity
Acknowledgements
The Renaissance TSA Biotin system is a registered trademark of PerkinElmer, Waltham, USA
Regulatory
For research purposes only

Applications of CD335 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunohistology - Paraffin 1
  1. 1 Clone EC1.1 is suitable for use on paraffin sections when fixed with zinc salts This product requires antigen retrieval using Renaissance TSA Biotin system®
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rat anti Human CD3:FITC MCA1477F 0.1 mg F*
Rat anti Human CD3:Pacific Blue® MCA1477PB 100 Tests/1ml F*
Mouse anti Bovine CD205:FITC MCA1651F 0.1 mg F
Mouse anti Bovine CD205 MCA1651GA 0.1 mg C F IP
Mouse anti Human CD16:FITC MCA5665F 0.1 mg F
Mouse anti Human CD16 MCA5665GA 0.1 mg F FN*
Mouse anti Sheep CD16-Like MCA919GA 0.1 mg C E F IP

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD335 antibody

  1. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
    Vet Res. 42: 37.
  2. Olsen, L. et al. (2013) Characterization of NCR1+ cells residing in lymphoid tissues in the gut of lambs indicates that the majority are NK cells
    Vet Res. 44:109.
  3. Goh, S. et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
    PLoS One. 11 (9): e0162571.

Further Reading

  1. Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.
    Vet Res. 41:4
  2. Storset, A. K. et. al. (2004) NKp46 defines a subset of bovine leukocytes with natural killer cell characteristics.
    Eur J Immunol. 34:669-676
  3. Westgaard, I.H. et al. (2004) Rat NKp46 activates natural killer cell cytotoxicity and is associated with FcepsilonRIgamma and CD3zeta.
    J Leukoc Biol. 76 (6): 1200-6.
  4. Boysen, P. et al. (2011) Natural killer cells in free-living Mus musculus have a primed phenotype.
    Mol Ecol. 20 (23): 5103-10.
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