TCR Alpha/Beta antibody | R73
Mouse anti Rat TCR alpha/beta:FITC
Mouse anti Rat TCR alpha/beta:Low Endotoxin
Mouse anti Rat TCR Alpha/Beta
- Product Type
- Monoclonal Antibody
- Clone
- R73
- Isotype
- IgG1
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| MCA453F | F | 0.1 mg | ||||
| MCA453EL | C F FN IP | 0.5 mg | ||||
| MCA453GA | C F IP | 0.1 mg | ||||
| MCA453G | C F IP | 0.25 mg |
Mouse anti Rat TCR alpha/beta antibody, clone R73 recognizes a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative.
Mouse anti Rat TCR alpha/beta antibody, clone R73 is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colic et al. 2010).
Mouse anti Rat TCR alpha/beta antibody, clone R73 is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colic et al. 2010).
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Monkey Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Rat T Blasts and Erythrocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20o only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of TCR Alpha/Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Functional Assays | |||
| Immunohistology - Frozen | 1/10 | 1/100 | |
| Immunoprecipitation | |||
| Flow Cytometry | 1/50 | 1/100 | |
| Immunohistology - Frozen | 1/10 | 1/100 | |
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 lymphocytes in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 100ul of whole blood or 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 100ul of whole blood or 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA1209F | 0.1 mg | F | ||
| Mouse IgG1 Negative Control:Low Endotoxin | MCA1209EL | 0.5 mg | F | ||
| Mouse IgG1 Negative Control | MCA1209 | 0.1 mg | F |
Product Specific References
References for TCR Alpha/Beta antibody
-
Hünig, T. et al. (1989) A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes.
J Exp Med. 169 (1): 73-86. -
Tomida, S. et al. (1994) Intercellular adhesion molecule-1 and leukocyte function-associated antigen-1 are involved in protection mediated by CD3+TCR alpha beta- T cells at the early stage after infection with Listeria monocytogenes in rats.
Int Immunol. 6 (7): 955-61. -
Colić, M. et al. (1996) Mechanisms involved in the binding of thymocytes to rat thymic dendritic cells.
Dev Immunol. 5 (1): 37-51. -
Kanellis, J. et al. (2010) JNK signalling in human and experimental renal ischaemia/reperfusion injury.
Nephrol Dial Transplant. 25: 2898-908. -
Nave, H. et al. (2008) Resistance of Janus kinase-2 dependent leptin signaling in natural killer (NK) cells: a novel mechanism of NK cell dysfunction in diet-induced obesity.
Endocrinology. 149: 3370-8. -
Tsuchida, M. et al. (1994) Identification of CD4- CD8- alpha beta T cells in the subarachnoid space of rats with experimental autoimmune encephalomyelitis. A possible route by which effector cells invade the lesions.
Immunology. 81 (3): 420-7. -
Matsumoto, Y. et al. (1994) Successful prevention and treatment of autoimmune encephalomyelitis by short-term administration of anti-T-cell receptor alpha beta antibody.
Immunology. 81 (1): 1-7. -
Pilipović, I. et al. (2010) Glucocorticoids, master modulators of the thymic catecholaminergic system?
Braz J Med Biol Res. 43 (3): 279-84. -
Milicevic, N.M. et al. (2005) T cells are required for the peripheral phase of B-cell maturation.
Immunology. 116: 308-17. -
Kenny, E. et al. (2000) Phenotypic analysis of peripheral CD4+ CD8+ T cells in the rat.
Immunology. 101: 178-84. -
Petrovic-Dergovic, D.M. et al. (2004) Somatostatin-14 alters the thymus size and relation among the thymocyte subpopulations in peripubertal rats.
Neuropeptides. 38: 25-34. -
Trinh, L. et al. (2008) The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
Mol Vis. 14: 1149-56. -
Khalife, S. et al. (2016) Relationship Between Pneumocystis carinii Burden and the Degree of Host Immunosuppression in an Airborne Transmission Experimental Model.
J Eukaryot Microbiol. 63 (3): 309-17. -
Bat, E. et al. (2013) Physical properties and erosion behavior of poly(trimethylene carbonate-co-ε-caprolactone) networks.
Macromol Biosci. 13 (5): 573-83. -
Jörns, A. et al. (2015) TNF-α Antibody Therapy in Combination With the T-Cell-Specific Antibody Anti-TCR Reverses the Diabetic Metabolic State in the LEW.1AR1-iddm Rat.
Diabetes. 64 (8): 2880-91. -
Ahn, M. et al. (2015) Immunohistochemical study of Krüppel-like factor 4 in the spinal cords of rats with experimental autoimmune encephalomyelitis.
Acta Histochem. 117 (6): 521-7. -
Jörns A et al. (2014) Anti-TCR therapy combined with fingolimod for reversal of diabetic hyperglycemia by β cell regeneration in the LEW.1AR1-iddm rat model of type 1 diabetes.
J Mol Med (Berl). 92 (7): 743-55.
