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TCR Alpha/Beta antibody | R73

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Anti Rat TCR Alpha/Beta Antibody, clone R73 thumbnail image 1 Anti Rat TCR Alpha/Beta Antibody, clone R73 thumbnail image 2 Anti Rat TCR Alpha/Beta Antibody, clone R73 thumbnail image 3 Anti Rat TCR Alpha/Beta Antibody, clone R73 thumbnail image 4
Anti Rat TCR Alpha/Beta Antibody, clone R73 gallery image 1
Figure A. Alexa Fluor 700 conjugated Mouse anti Rat CD3 (MCA772A700) and FITC conjugated Mouse IgG1 isotype control (MCA1209F). Figure B. Alexa Fluor 700 conjugated Mouse anti Rat CD3 (MCA772A700) and FITC conjugated Mouse anti Rat TCR αβ (MCA453F). All experiments performed on red cell lysed rat blood gated on lymphoid cells in the presence of 10% rat serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Rat TCR Alpha/Beta Antibody, clone R73 gallery image 2
Figure A. RPE conjugated mouse anti rat TCRa/b (MCA453PE) and FITC conjugated mouse IgG1 isotype control (MCA1209F). Figure B. RPE conjugated mouse anti rat TCRa/b (MCA453PE) and FITC conjugated mouse anti rat CD31 (MCA1334F). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat TCR Alpha/Beta Antibody, clone R73 gallery image 3
Figure A. FITC conjugated mouse anti rat CD31 (MCA1334F) and RPE conjugated mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated mouse anti rat CD31 (MCA1334F) and RPE conjugated mouse anti rat TCRa/b (MCA453PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat TCR Alpha/Beta Antibody, clone R73 gallery image 4
Published customer image:
Mouse anti Rat TCR alpha/beta antibody, clone R73 (MCA453G) used to identify infiltrating T cells in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
T‐cell infiltration in day 14 nephrotoxic serum nephritis (NTN). Disease was induced in groups of 8 WKY rats which were treated with GS‐444217 (drug; NTN‐D) or vehicle (NTN‐V) alone. A‐C, Immunostaining for R73+ T cells: A, normal rat kidney; B, vehicle treated disease shows a substantial infiltrate of T cells in glomeruli and the interstitium; C, drug treatment shows a marked reduction in T‐cell infiltration in both compartments.

From: Amos LA, Ma FY, Tesch GH, Liles JT, Breckenridge DG, Nikolic-Paterson DJ, Han Y.
ASK1 inhibitor treatment suppresses p38/JNK signalling with reduced kidney inflammation and fibrosis in rat crescentic glomerulonephritis.
J Cell Mol Med. 2018 Sep;22(9):4522-4533.
doi: 10.1111/jcmm.13705.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Rat TCR alpha/beta:FITC

Mouse anti Rat TCR alpha/beta:Low Endotoxin

Mouse anti Rat TCR Alpha/Beta

Product Type
Monoclonal Antibody
Clone
R73
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA453F
Datasheet
F
SDS
0.1 mg loader

MCA453EL
Datasheet
C F FN IP
SDS
0.5 mg loader

MCA453G
Datasheet
C F IP
SDS
0.25 mg loader

MCA453GA
Datasheet
C F IP
SDS
0.1 mg loader

Mouse anti Rat TCR alpha/beta antibody, clone R73 recognizes a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative.

Mouse anti Rat TCR alpha/beta antibody, clone R73 is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colic et al. 2010).

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Monkey
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Rat T Blasts and Erythrocytes.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
Store at -20o only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of TCR Alpha/Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Flow Cytometry 1/50 1/100
Functional Assays
Immunohistology - Frozen 1/10 1/100
Immunoprecipitation
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1/10 1/100
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 lymphocytes in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 100ul of whole blood or 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 100ul of whole blood or 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
Mouse IgG1 Negative Control:Low Endotoxin MCA1209EL F 0.5 mg loader
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for TCR Alpha/Beta antibody

  1. Hünig, T. et al. (1989) A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes.
    J Exp Med. 169 (1): 73-86.
  2. Tomida, S. et al. (1994) Intercellular adhesion molecule-1 and leukocyte function-associated antigen-1 are involved in protection mediated by CD3+TCR alpha beta- T cells at the early stage after infection with Listeria monocytogenes in rats.
    Int Immunol. 6 (7): 955-61.
  3. Colić, M. et al. (1996) Mechanisms involved in the binding of thymocytes to rat thymic dendritic cells.
    Dev Immunol. 5 (1): 37-51.
  4. Kanellis, J. et al. (2010) JNK signalling in human and experimental renal ischaemia/reperfusion injury.
    Nephrol Dial Transplant. 25: 2898-908.
  5. Nave, H. et al. (2008) Resistance of Janus kinase-2 dependent leptin signaling in natural killer (NK) cells: a novel mechanism of NK cell dysfunction in diet-induced obesity.
    Endocrinology. 149: 3370-8.
  6. Tsuchida, M. et al. (1994) Identification of CD4- CD8- alpha beta T cells in the subarachnoid space of rats with experimental autoimmune encephalomyelitis. A possible route by which effector cells invade the lesions.
    Immunology. 81 (3): 420-7.
  7. Matsumoto, Y. et al. (1994) Successful prevention and treatment of autoimmune encephalomyelitis by short-term administration of anti-T-cell receptor alpha beta antibody.
    Immunology. 81 (1): 1-7.
  8. Pilipović, I. et al. (2010) Glucocorticoids, master modulators of the thymic catecholaminergic system?
    Braz J Med Biol Res. 43 (3): 279-84.
  9. Milicevic, N.M. et al. (2005) T cells are required for the peripheral phase of B-cell maturation.
    Immunology. 116: 308-17.
  10. Kenny, E. et al. (2000) Phenotypic analysis of peripheral CD4+ CD8+ T cells in the rat.
    Immunology. 101: 178-84.
  11. Petrovic-Dergovic, D.M. et al. (2004) Somatostatin-14 alters the thymus size and relation among the thymocyte subpopulations in peripubertal rats.
    Neuropeptides. 38: 25-34.
  12. Trinh, L. et al. (2008) The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
    Mol Vis. 14: 1149-56.
  13. Khalife, S. et al. (2016) Relationship Between Pneumocystis carinii Burden and the Degree of Host Immunosuppression in an Airborne Transmission Experimental Model.
    J Eukaryot Microbiol. 63 (3): 309-17.
  14. Bat, E. et al. (2013) Physical properties and erosion behavior of poly(trimethylene carbonate-co-ε-caprolactone) networks.
    Macromol Biosci. 13 (5): 573-83.
  15. Jörns, A. et al. (2015) TNF-α Antibody Therapy in Combination With the T-Cell-Specific Antibody Anti-TCR Reverses the Diabetic Metabolic State in the LEW.1AR1-iddm Rat.
    Diabetes. 64 (8): 2880-91.
  16. Ahn, M. et al. (2015) Immunohistochemical study of Krüppel-like factor 4 in the spinal cords of rats with experimental autoimmune encephalomyelitis.
    Acta Histochem. 117 (6): 521-7.
  17. Jörns A et al. (2014) Anti-TCR therapy combined with fingolimod for reversal of diabetic hyperglycemia by β cell regeneration in the LEW.1AR1-iddm rat model of type 1 diabetes.
    J Mol Med (Berl). 92 (7): 743-55.
  18. Amos, L.A. et al. (2018) ASK1 inhibitor treatment suppresses p38/JNK signalling with reduced kidney inflammation and fibrosis in rat crescentic glomerulonephritis.
    J Cell Mol Med. 22 (9): 4522-33.

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