TCR Alpha/Beta antibody | R73

Mouse anti Rat TCR Alpha/Beta

Product Type
Monoclonal Antibody
Clone
R73
Isotype
IgG1
Specificity
TCR Alpha/Beta

Product Code Applications Pack Size List Price Your Price Qty
MCA453G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.25 mg loader
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loader
MCA453GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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loader
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Mouse anti Rat TCR alpha/beta antibody, clone R73 recognizes a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative.

Mouse anti Rat TCR alpha/beta antibody, clone R73 is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colic et al. 2010).

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Monkey
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat T Blasts and Erythrocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1/10 1/100
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 100ul of whole blood or 106 cells in 100ul.

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Description Mouse IgG1 Negative Control

References for TCR Alpha/Beta antibody

  1. Ariki, S.G. and Roux, M.E. (1995) T lymphocyte subpopulations in the intestinal villi of immune deficient rats.
    In Recent Advances in Mucosal Immunology. Edited by McGhee, J., Mestecky, J., Tlaskalova, H. and Sterzl, J.
  2. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.
  3. Hünig, T. et al. (1989) A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes.
    J Exp Med. 169 (1): 73-86.
  4. Tomida, S. et al. (1994) Intercellular adhesion molecule-1 and leukocyte function-associated antigen-1 are involved in protection mediated by CD3+TCR alpha beta- T cells at the early stage after infection with Listeria monocytogenes in rats.
    Int Immunol. 6 (7): 955-61.
  5. Colić, M. et al. (1996) Mechanisms involved in the binding of thymocytes to rat thymic dendritic cells.
    Dev Immunol. 5 (1): 37-51.
  6. Kanellis, J. et al. (2010) JNK signalling in human and experimental renal ischaemia/reperfusion injury.
    Nephrol Dial Transplant. 25: 2898-908.
  7. Nave, H. et al. (2008) Resistance of Janus kinase-2 dependent leptin signaling in natural killer (NK) cells: a novel mechanism of NK cell dysfunction in diet-induced obesity.
    Endocrinology. 149: 3370-8.
  8. Tsuchida, M. et al. (1994) Identification of CD4- CD8- alpha beta T cells in the subarachnoid space of rats with experimental autoimmune encephalomyelitis. A possible route by which effector cells invade the lesions.
    Immunology. 81 (3): 420-7.
  9. View The Latest Product References
  10. Matsumoto, Y. et al. (1994) Successful prevention and treatment of autoimmune encephalomyelitis by short-term administration of anti-T-cell receptor alpha beta antibody.
    Immunology. 81 (1): 1-7.
  11. Pilipović, I. et al. (2010) Glucocorticoids, master modulators of the thymic catecholaminergic system?
    Braz J Med Biol Res. 43 (3): 279-84.
  12. Milicevic, N.M. et al. (2005) T cells are required for the peripheral phase of B-cell maturation.
    Immunology. 116: 308-17.
  13. Kenny, E. et al. (2000) Phenotypic analysis of peripheral CD4+ CD8+ T cells in the rat.
    Immunology. 101: 178-84.
  14. Petrovic-Dergovic, D.M. et al. (2004) Somatostatin-14 alters the thymus size and relation among the thymocyte subpopulations in peripubertal rats.
    Neuropeptides. 38: 25-34.
  15. Trinh, L. et al. (2008) The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
    Mol Vis. 14: 1149-56.
  16. Khalife, S. et al. (2016) Relationship Between Pneumocystis carinii Burden and the Degree of Host Immunosuppression in an Airborne Transmission Experimental Model.
    J Eukaryot Microbiol. 63 (3): 309-17.
  17. Bat, E. et al. (2013) Physical properties and erosion behavior of poly(trimethylene carbonate-co-ε-caprolactone) networks.
    Macromol Biosci. 13 (5): 573-83.
  18. Jörns, A. et al. (2015) TNF-α Antibody Therapy in Combination With the T-Cell-Specific Antibody Anti-TCR Reverses the Diabetic Metabolic State in the LEW.1AR1-iddm Rat.
    Diabetes. 64 (8): 2880-91.
  19. Ahn, M. et al. (2015) Immunohistochemical study of Krüppel-like factor 4 in the spinal cords of rats with experimental autoimmune encephalomyelitis.
    Acta Histochem. 117 (6): 521-7.
  20. Jörns A et al. (2014) Anti-TCR therapy combined with fingolimod for reversal of diabetic hyperglycemia by β cell regeneration in the LEW.1AR1-iddm rat model of type 1 diabetes.
    J Mol Med (Berl). 92 (7): 743-55.
  21. Amos, L.A. et al. (2018) ASK1 inhibitor treatment suppresses p38/JNK signalling with reduced kidney inflammation and fibrosis in rat crescentic glomerulonephritis.
    J Cell Mol Med. 22 (9): 4522-33.
  22. Koppe, C. et al. (2021) Local Inflammatory Response after Intramuscularly Implantation of Anti-Adhesive Plasma-Fluorocarbon-Polymer Coated Ti6AI4V Discs in Rats.
    Polymers (Basel). 13 (16): 2684.
  23. Schmiedl, A. et al. (2021) Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
    Cell Tissue Res. Oct 04 [Epub ahead of print].
  24. Köhler, R. et al. (2022) Association of systemic antibody response against polyethylene terephthalate with inflammatory serum cytokine profile following implantation of differently coated vascular prostheses in a rat animal model.
    J Biomed Mater Res A. 110 (1): 52-63.

Flow Cytometry

Immunofluorescence

RRID
AB_322435
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