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OX-62 antibody | OX-62

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Anti Rat OX-62 Antibody, clone OX-62 thumbnail image 1 Anti Rat OX-62 Antibody, clone OX-62 thumbnail image 2 Anti Rat OX-62 Antibody, clone OX-62 thumbnail image 3 Anti Rat OX-62 Antibody, clone OX-62 thumbnail image 4 Anti Rat OX-62 Antibody, clone OX-62 thumbnail image 5
Anti Rat OX-62 Antibody, clone OX-62 gallery image 1
Rat splenocytes stained with phycoerythrin conjugated Mouse anti Rat OX-62 (MCA1029PE) in red filled, with PE conjugated Mouse IgG1 isotype control (MCA1209PE) blue line.
Anti Rat OX-62 Antibody, clone OX-62 gallery image 2
Published customer image:
Mouse anti Rat OX-62 antibody, clone OX-62 (MCA1029) used to detect OX-62 expressing cells in corneal allograft sections by immunohistochemistry on cryostat sections.
Image caption:
Analysis of innate immune cells into a corneal graft. Ox-62+ DC and CD163+ macrophages were stained at the time points of corneal allograft rejection and calculated within the graft. Additionally CD161+ cells were counted within rejected corneal allografts to finally prove the efficacy of the depletion protocol in the peripheral tissue. Representative histological staining is shown for Ox-62 (A), CD163 (C), and CD161 (E) in NK deficient and control animals. B: No statistical difference was observed for infiltrating Ox-62+ DC. D: CD163+ macrophages infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p<0.01). F: No CD161+ cells were stained in 3.2.3-treated recipients when compared to control treated control animals (**p<0.001).

From: Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D,Reinhard T.
NK cell depletion delays corneal allograft rejection in baby rats. Mol Vis. 2010 Oct 2;16:1928-35.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Rat OX-62 Antibody, clone OX-62 gallery image 3
Published customer image:
Mouse anti Rat OX-62 antibody, clone OX-62 (MCA1029) used to evaluate OX-62 expression on rat dendritic cells by flow cytometry.
Image caption:
The expression of OX62+DCs and OX62+CD4+SIRP+DCs of each groups at various development stages (Mean ± SD). (a) The expression level of OX62+DCs of each group at different stages. (b) The expression level of OX62+CD4+SIRP+ DCs of each group at different stages. (c) Single-cell suspensions of the total PP-DCs in rats were identified by OX62. The difference of OX62+DC among groups at different development stages was not significant. (F = 3.0, 0.587, 3.267, and 1.471, resp.; P>.05). Significant growth occurred in LR group for the number of OX62+CD4+SIRP+DCs at age week 3. Levels of OX62+CD4+SIRP+DC subsets at every age were highly significant in LR group and BR group compared with AR group. Furthermore, the positive cell numbers were higher in LR group than in BR group. The positive cell numbers kept stable in LR group at various ages while the positive cells number increased with age in the other two groups. Significant numbers of OX62+CD4+SIRP+DCs were found in LR and BR group at various ages (Figures 1(b) and 1(c)) compared with AR group (A: BR at W3, B: AR at W3, C: LR at W3, D: BR at W5, E: AR at W5, F: LR at W5, G: BR at W7, H: AR at W7, I: LR at W7, J: BR at W11, K: AR at W11, L: LR at W11). *P
From: Yi-Jun Zhou, Juan Gao, Hua-Mei Yang, Xiang-Liang Yuan, Tong-Xin Chen, and Zhen-Juan He.
The Role of the Lactadherin in Promoting Intestinal DCs Development In Vivo and Vitro,” Clinical and Developmental Immunology, vol. 2010, Article ID 357541, 9 pages, 2010.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Rat OX-62 Antibody, clone OX-62 gallery image 4
Published customer image:Mouse anti Rat OX062 antibody, clone OX-62 (MCA1029G) used to identify OX-62 expressing cells in cytospins of cultured bone marrow cells by immunocytochemistry.
Image caption:
Morphology and immunostaining of IL-4 DC and IL-10 DC. Rat BMDC were isolated from cell clusters on day 6 of culture (A). Cells prepared on cytospin slides stained positive for monoclonal antibodies Ox62 (rat DC marker) (B), Ox6 (MHC class II) (C), and CD68 (D). Shown are representative IL-4 DC results, which are similar to those for IL-10 DC. Magnification: ×200 (A, C, D) and ×400 (B).

From: Tiurbe G, Matuschek A, Kämmerer U, Schneider M, Thiede A, Ulrichs K, Otto C.
Inhibitory effects of rat bone marrow-derived dendritic cells on naïve and alloantigen-specific CD4+ T cells: a comparison between dendritic cells generated with GM-CSF plus IL-4 and dendritic cells generated with GM-CSF plus IL-10.
BMC Res Notes. 2009 Jan 23;2:12.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Rat OX-62 Antibody, clone OX-62 gallery image 5
Published customer image:
Mouse anti Rat OX-62 antibody, clone OX-62 used to evaluate OX-62 expression in cultured bone marrow cells by flow cytometry.
Image caption:
IL-4 DC and IL-10 DC exhibit no obvious differences in their phenotype. IL-4 DC and IL-10 DC and mature splenic DC (sDC) coexpressed Ox62 and CD68, whereas macrophages generated in the presence of M-CSF (5 μg/ml) were only positive for CD68. Broken lines indicate background staining obtained using an irrelevant isotype control. The first number represents the percentage of cells staining positive for the indicated marker and the second number represents the mean fluorescence intensity. The results shown are representative for 4 independent flow cytometric analyses.

From:From: Tiurbe G, Matuschek A, Kämmerer U, Schneider M, Thiede A, Ulrichs K, Otto C.
Inhibitory effects of rat bone marrow-derived dendritic cells on naïve and alloantigen-specific CD4+ T cells: a comparison between dendritic cells generated with GM-CSF plus IL-4 and dendritic cells generated with GM-CSF plus IL-10.
BMC Res Notes. 2009 Jan 23;2:12.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Rat OX-62

Product Type
Monoclonal Antibody
Clone
OX-62
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1029G
Datasheet
C F IP WB
SDS
0.25 mg loader

MCA1029GA
Datasheet
C F IP
SDS
0.1 mg loader

Mouse anti Rat OX-62 antibody, clone OX-62 recognizes the OX-62 antigen, also known as rat alpha E integrin or CD103. OX-62 appears as an 1150 amino acid, ~130 kDa single pass type I transmembrane protein expressed by intestinal dendritic cells, dendritic epidermal T cells, intraepithelial lymphocytes in the small intestine and by cells of dendritic morphology in lymphoid organs, at sites where gamma delta T cells are present.

Product Details

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Density gradient enriched PVG rat thoracic duct dendritic cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells of BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O88341
Regulatory
For research purposes only

Applications of OX-62 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1/25 1/100
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader

Application Based External Images

Flow Cytometry

Immunocytochemistry

Immunohistology - Frozen

Product Specific References

References for OX-62 antibody

  1. Brenan, M. & Puklavec, M. (1992) The MRC OX-62 antigen: a useful marker in the purification of rat veiled cells with the biochemical properties of an integrin.
    J Exp Med. 175 (6): 1457-65.
  2. Brenan, M. & Rees, D.J. (1997) Sequence analysis of rat integrin alpha E1 and alpha E2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph.
    Eur J Immunol. 27 (11): 3070-9.
  3. Kostulas, N. et al. (2002) Dendritic cells are present in ischemic brain after permanent middle cerebral artery occlusion in the rat.
    Stroke. 33 (4): 1129-34.
  4. Rival, C. et al. (2006) Identification of a dendritic cell population in normal testis and in chronically inflamed testis of rats with autoimmune orchitis.
    Cell Tissue Res. 324 (2): 311-8.
  5. Tsuchiya, T. et al. (2002) Dendritic cell involvement in pulmonary granuloma formation elicited by bacillus calmette-guérin in rats.
    Am J Respir Crit Care Med. 165 (12): 1640-6.
  6. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
    J Neuroimmunol. 209 (1-2): 16-25.
  7. Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
    Mol Vis. 16: 1928-35.
  8. Baca Jones, C.C. et al. (2009) Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells.
    Virology. 388: 78-90.
  9. Zhou, Y.J. et al. (2010) The role of the lactadherin in promoting intestinal DCs development in vivo and vitro.
    Clin Dev Immunol. 2010: 357541.
  10. Aiello, S. et al. (2000) Thymic Dendritic Cells Express Inducible Nitric Oxide Synthase and Generate Nitric Oxide in Response to Self- and Alloantigens
    J Immunol. 164: 4649-58.
  11. Camelo, S. et al. (2003) Local retention of soluble antigen by potential antigen-presenting cells in the anterior segment of the eye.
    Invest Ophthalmol Vis Sci. 44: 5212-9.
  12. Camelo, S. et al. (2004) The distribution of antigen in lymphoid tissues following its injection into the anterior chamber of the rat eye.
    J Immunol. 172: 5388-95.
  13. Henry, F. et al. (1999) Antigen-presenting cells that phagocytose apoptotic tumor-derived cells are potent tumor vaccines.
    Cancer Res. 59: 3329-32.
  14. Liu, L. et al. (1998) Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression.
    J Immunol. 161: 1146-55.
  15. O'Sullivan, N.L. et al. (2001) Lymphocyte lineages at mucosal effector sites: rat salivary glands.
    J Immunol. 166: 5522-9.
  16. Penttila, I.A. et al. (2003) Maternal milk regulation of cell infiltration and interleukin 18 in the intestine of suckling rat pups.
    Gut. 52: 1579-86.
  17. Stephens, L.A. et al. (2004) Phenotypic characterization of regulatory CD4+CD25+ T cells in rats.
    Int Immunol. 16: 365-75.
  18. Sugawara, I. et al. (2004) Pathological and immunological profiles of rat tuberculosis.
    Int J Exp Pathol. 85: 125-34.
  19. Tiurbe, G. et al. (2009) Inhibitory effects of rat bone marrow-derived dendritic cells on naïve and alloantigen-specific CD4+ T cells: a comparison between dendritic cells generated with GM-CSF plus IL-4 and dendritic cells generated with GM-CSF plus IL-10.
    BMC Res Notes. 2: 12.
  20. Chen-Woan, M. et al. (1995) A new protocol for the propagation of dendritic cells from rat bone marrow using recombinant GM-CSF, and their quantification using the mAb OX-62.
    J Immunol Methods. 178 (2): 157-71.
  21. Stojić-Vukanić, Z. et al. (2016) Estradiol enhances capacity of TLR-matured splenic dendritic cells to polarize CD4+ lymphocytes into IL-17/GM-CSF-producing cells in vitro.
    Int Immunopharmacol. 40: 244-253.
  22. Varas, A. et al. (1998) Interleukin-7 influences the development of thymic dendritic cells.
    Blood. 92: 93-100.
  23. Bufan, B. et al. (2015) Aging Impairs Endocytic Capacity Of Splenic Dendritic Cells From Dark Agouti Rats And Alters Their Response To TLR4 Stimulation
    Acta Veterinaria. Volume 65, Issue 1, Pages 30–55.
  24. Syrjälä, S. et al. (2015) Donor Heart Treatment With COMP-Ang1 Limits Ischemia-Reperfusion Injury and Rejection of Cardiac Allografts.
    Am J Transplant. 15 (8): 2075-84.
  25. Palin, N.K. et al. (2017) Activin inhibition limits early innate immune response in rat kidney allografts-a pilot study.
    Transpl Int. 30 (1): 96-107.
  26. Raissadati, A. et al. (2017) Vascular Endothelial Growth Factor-B Overexpressing Hearts Are Not Protected From Transplant-Associated Ischemia-Reperfusion Injury.
    Exp Clin Transplant. 15 (2): 203-12.
  27. Stojić-Vukanić, Z. et al. (2019) Age and sex determine CD4+ T cell stimulatory and polarizing capacity of rat splenic dendritic cells.
    Biogerontology. Oct 23 [Epub ahead of print].
  28. Lauzon-Joset, J.F. et al. (2020) Oestrogen amplifies pre-existing atopy-associated Th2 bias in an experimental asthma model.
    Clin Exp Allergy. 50 (3): 391-400.
  29. Pilipović, I. et al. (2019) Noradrenaline modulates CD4+ T cell priming in rat experimental autoimmune encephalomyelitis: a role for the α1-adrenoceptor.
    Immunol Res. 67 (2-3): 223-240.

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