OX-62 antibody | OX-62
Mouse anti Rat OX-62 antibody, clone OX-62 (MCA1029) used to detect OX-62 expressing cells in corneal allograft sections by immunohistochemistry on cryostat sections.
Image caption:
Analysis of innate immune cells into a corneal graft. Ox-62+ DC and CD163+ macrophages were stained at the time points of corneal allograft rejection and calculated within the graft. Additionally CD161+ cells were counted within rejected corneal allografts to finally prove the efficacy of the depletion protocol in the peripheral tissue. Representative histological staining is shown for Ox-62 (A), CD163 (C), and CD161 (E) in NK deficient and control animals. B: No statistical difference was observed for infiltrating Ox-62+ DC. D: CD163+ macrophages infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p<0.01). F: No CD161+ cells were stained in 3.2.3-treated recipients when compared to control treated control animals (**p<0.001).
From: Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D,Reinhard T.
NK cell depletion delays corneal allograft rejection in baby rats. Mol Vis. 2010 Oct 2;16:1928-35.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat OX-62 antibody, clone OX-62 (MCA1029) used to evaluate OX-62 expression on rat dendritic cells by flow cytometry.
Image caption:
The expression of OX62+DCs and OX62+CD4+SIRP+DCs of each groups at various development stages (Mean ± SD). (a) The expression level of OX62+DCs of each group at different stages. (b) The expression level of OX62+CD4+SIRP+ DCs of each group at different stages. (c) Single-cell suspensions of the total PP-DCs in rats were identified by OX62. The difference of OX62+DC among groups at different development stages was not significant. (F = 3.0, 0.587, 3.267, and 1.471, resp.; P>.05). Significant growth occurred in LR group for the number of OX62+CD4+SIRP+DCs at age week 3. Levels of OX62+CD4+SIRP+DC subsets at every age were highly significant in LR group and BR group compared with AR group. Furthermore, the positive cell numbers were higher in LR group than in BR group. The positive cell numbers kept stable in LR group at various ages while the positive cells number increased with age in the other two groups. Significant numbers of OX62+CD4+SIRP+DCs were found in LR and BR group at various ages (Figures 1(b) and 1(c)) compared with AR group (A: BR at W3, B: AR at W3, C: LR at W3, D: BR at W5, E: AR at W5, F: LR at W5, G: BR at W7, H: AR at W7, I: LR at W7, J: BR at W11, K: AR at W11, L: LR at W11). *P
From: Yi-Jun Zhou, Juan Gao, Hua-Mei Yang, Xiang-Liang Yuan, Tong-Xin Chen, and Zhen-Juan He.
The Role of the Lactadherin in Promoting Intestinal DCs Development In Vivo and Vitro,” Clinical and Developmental Immunology, vol. 2010, Article ID 357541, 9 pages, 2010.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
Morphology and immunostaining of IL-4 DC and IL-10 DC. Rat BMDC were isolated from cell clusters on day 6 of culture (A). Cells prepared on cytospin slides stained positive for monoclonal antibodies Ox62 (rat DC marker) (B), Ox6 (MHC class II) (C), and CD68 (D). Shown are representative IL-4 DC results, which are similar to those for IL-10 DC. Magnification: ×200 (A, C, D) and ×400 (B).
From: Tiurbe G, Matuschek A, Kämmerer U, Schneider M, Thiede A, Ulrichs K, Otto C.
Inhibitory effects of rat bone marrow-derived dendritic cells on naïve and alloantigen-specific CD4+ T cells: a comparison between dendritic cells generated with GM-CSF plus IL-4 and dendritic cells generated with GM-CSF plus IL-10.
BMC Res Notes. 2009 Jan 23;2:12.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat OX-62 antibody, clone OX-62 used to evaluate OX-62 expression in cultured bone marrow cells by flow cytometry.
Image caption:
IL-4 DC and IL-10 DC exhibit no obvious differences in their phenotype. IL-4 DC and IL-10 DC and mature splenic DC (sDC) coexpressed Ox62 and CD68, whereas macrophages generated in the presence of M-CSF (5 μg/ml) were only positive for CD68. Broken lines indicate background staining obtained using an irrelevant isotype control. The first number represents the percentage of cells staining positive for the indicated marker and the second number represents the mean fluorescence intensity. The results shown are representative for 4 independent flow cytometric analyses.
From:From: Tiurbe G, Matuschek A, Kämmerer U, Schneider M, Thiede A, Ulrichs K, Otto C.
Inhibitory effects of rat bone marrow-derived dendritic cells on naïve and alloantigen-specific CD4+ T cells: a comparison between dendritic cells generated with GM-CSF plus IL-4 and dendritic cells generated with GM-CSF plus IL-10.
BMC Res Notes. 2009 Jan 23;2:12.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat OX-62 antibody, clone OX-62 recognizes the OX-62 antigen, also known as rat alpha E integrin or CD103. OX-62 appears as an 1150 amino acid, ~130 kDa single pass type I transmembrane protein expressed by intestinal dendritic cells, dendritic epidermal T cells, intraepithelial lymphocytes in the small intestine and by cells of dendritic morphology in lymphoid organs, at sites where gamma delta T cells are present.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Density gradient enriched PVG rat thoracic duct dendritic cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells of BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- O88341
- Regulatory
- For research purposes only
Applications of OX-62 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/100 | |
| Immunohistology - Frozen | 1/25 | 1/100 | |
| Immunoprecipitation | |||
| Western Blotting | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
|
| Mouse IgG1 Negative Control:RPE | MCA1209PE | F | 100 Tests |
|
Product Specific References
References for OX-62 antibody
-
Brenan, M. & Puklavec, M. (1992) The MRC OX-62 antigen: a useful marker in the purification of rat veiled cells with the biochemical properties of an integrin.
J Exp Med. 175 (6): 1457-65. -
Brenan, M. & Rees, D.J. (1997) Sequence analysis of rat integrin alpha E1 and alpha E2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph.
Eur J Immunol. 27 (11): 3070-9. -
Kostulas, N. et al. (2002) Dendritic cells are present in ischemic brain after permanent middle cerebral artery occlusion in the rat.
Stroke. 33 (4): 1129-34. -
Rival, C. et al. (2006) Identification of a dendritic cell population in normal testis and in chronically inflamed testis of rats with autoimmune orchitis.
Cell Tissue Res. 324 (2): 311-8. -
Tsuchiya, T. et al. (2002) Dendritic cell involvement in pulmonary granuloma formation elicited by bacillus calmette-guérin in rats.
Am J Respir Crit Care Med. 165 (12): 1640-6. -
Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
J Neuroimmunol. 209 (1-2): 16-25. -
Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 16: 1928-35. -
Baca Jones, C.C. et al. (2009) Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells.
Virology. 388: 78-90. -
Zhou, Y.J. et al. (2010) The role of the lactadherin in promoting intestinal DCs development in vivo and vitro.
Clin Dev Immunol. 2010: 357541. -
Aiello, S. et al. (2000) Thymic Dendritic Cells Express Inducible Nitric Oxide Synthase and Generate Nitric Oxide in Response to Self- and Alloantigens
J Immunol. 164: 4649-58. -
Camelo, S. et al. (2003) Local retention of soluble antigen by potential antigen-presenting cells in the anterior segment of the eye.
Invest Ophthalmol Vis Sci. 44: 5212-9. -
Camelo, S. et al. (2004) The distribution of antigen in lymphoid tissues following its injection into the anterior chamber of the rat eye.
J Immunol. 172: 5388-95. -
Henry, F. et al. (1999) Antigen-presenting cells that phagocytose apoptotic tumor-derived cells are potent tumor vaccines.
Cancer Res. 59: 3329-32. -
Liu, L. et al. (1998) Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression.
J Immunol. 161: 1146-55. -
O'Sullivan, N.L. et al. (2001) Lymphocyte lineages at mucosal effector sites: rat salivary glands.
J Immunol. 166: 5522-9. -
Penttila, I.A. et al. (2003) Maternal milk regulation of cell infiltration and interleukin 18 in the intestine of suckling rat pups.
Gut. 52: 1579-86. -
Stephens, L.A. et al. (2004) Phenotypic characterization of regulatory CD4+CD25+ T cells in rats.
Int Immunol. 16: 365-75. -
Sugawara, I. et al. (2004) Pathological and immunological profiles of rat tuberculosis.
Int J Exp Pathol. 85: 125-34. -
Tiurbe, G. et al. (2009) Inhibitory effects of rat bone marrow-derived dendritic cells on naïve and alloantigen-specific CD4+ T cells: a comparison between dendritic cells generated with GM-CSF plus IL-4 and dendritic cells generated with GM-CSF plus IL-10.
BMC Res Notes. 2: 12. -
Chen-Woan, M. et al. (1995) A new protocol for the propagation of dendritic cells from rat bone marrow using recombinant GM-CSF, and their quantification using the mAb OX-62.
J Immunol Methods. 178 (2): 157-71. -
Stojić-Vukanić, Z. et al. (2016) Estradiol enhances capacity of TLR-matured splenic dendritic cells to polarize CD4+ lymphocytes into IL-17/GM-CSF-producing cells in vitro.
Int Immunopharmacol. 40: 244-253. -
Varas, A. et al. (1998) Interleukin-7 influences the development of thymic dendritic cells.
Blood. 92: 93-100. -
Bufan, B. et al. (2015) Aging Impairs Endocytic Capacity Of Splenic Dendritic Cells From Dark Agouti Rats And Alters Their Response To TLR4 Stimulation
Acta Veterinaria. Volume 65, Issue 1, Pages 30–55. -
Syrjälä, S. et al. (2015) Donor Heart Treatment With COMP-Ang1 Limits Ischemia-Reperfusion Injury and Rejection of Cardiac Allografts.
Am J Transplant. 15 (8): 2075-84. -
Palin, N.K. et al. (2017) Activin inhibition limits early innate immune response in rat kidney allografts-a pilot study.
Transpl Int. 30 (1): 96-107. -
Raissadati, A. et al. (2017) Vascular Endothelial Growth Factor-B Overexpressing Hearts Are Not Protected From Transplant-Associated Ischemia-Reperfusion Injury.
Exp Clin Transplant. 15 (2): 203-12. -
Stojić-Vukanić, Z. et al. (2019) Age and sex determine CD4+ T cell stimulatory and polarizing capacity of rat splenic dendritic cells.
Biogerontology. Oct 23 [Epub ahead of print].
