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Neurofilament H antibody | RT97

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Anti Rat Neurofilament 200kDa Antibody, clone RT97 gallery image 1
Published customer image:
Mouse anti Rat Neurofilament 200kDa antibody, clone RT97 (MCA1321) used to label neurons in rat brain sections by immunofluorescence.
Image caption:
CRMP2 expression and phosphorylation is detected in L6 DRG neuron subpopulations. Micrographs of a 10 μm section of adult L6 DRG from animals having received a spared nerve injury and immunostained with antibodies against NF200 and NaV1.8 as indicated. Micrographs are representative of N = 4 animals with 8 independent slices per animal per staining combination; ipsi = ipsilateral (injured side) and contra = contralateral (non-injured side).

From: Moutal A, Ji Y, Bellampalli SS, Khanna R.
Differential expression of Cdk5-phosphorylated CRMP2 following a spared nerve injury.
Mol Brain. 2020 Jun 22;13(1):97.
doi: 10.1186/s13041-020-00633-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Rat Neurofilament 200kDa

Product Type
Monoclonal Antibody
Clone
RT97
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1321GA
Datasheet
C P
SDS
0.1 mg loader

Mouse anti Rat Neurofilament 200kDa antibody, clone RT97 recognizes the 200kDa neurofilament protein in a range of species. Mouse anti Rat Neurofilament 200kDa antibody, clone RT97 stains various tumors including phaeochromocytoma, paraganglioma and ganglioneuroblastoma.

Mouse anti Rat Neurofilament 200kDa antibody, clone RT97 also recognizes a phosphorylation dependent epitope on fetal tau, Alzheimer's paired helical filament-tau and on microtubule associated protein 1B (MAP1B) by western blotting, however similar reactivity was not apparent in immunohistochemical studies (Johnstone et al. 1997).

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Reptile Expected from Sequence
Human
Chicken
Pig
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by hydrophobic interaction chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
Immunogen
Triton X-100-insoluble rat brain protein.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P16884
Entrez Gene
Nefh
Regulatory
For research purposes only

Applications of Neurofilament H antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin 1/50 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Brain or spinal cord
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Immunoblotting

Immunofluorescence

Western Blotting

Product Specific References

References for Neurofilament H antibody

  1. Weber, K. et al. (1983) Neurofilaments, a subclass of intermediate filaments: structure and expression.
    Cold Spring Harb Symp Quant Biol. 48 Pt 2: 717-29.
  2. Anderton, B.H. et al. (1982) Monoclonal antibodies show that neurofibrillary tangles and neurofilaments share antigenic determinants.
    Nature. 298 (5869): 84-6.
  3. Johnstone, M. et al. (1997) The neurofilament antibody RT97 recognises a developmentally regulated phosphorylation epitope on microtubule-associated protein 1B.
    J Anat. 191 ( Pt 2): 229-44.
  4. Tonge, D.A. et al. (1996) Expression of a developmentally regulated, phosphorylated isoform of microtubule-associated protein 1B in sprouting and regenerating axons in vitro.
    Neuroscience. 73 (2): 541-51.
  5. Sann, H. et al. (1995) RT97: a marker for capsaicin-insensitive sensory endings in the rat skin.
    Cell Tissue Res. 282 (1): 155-61.
  6. Veeranna, et al. (2008) Neurofilament tail phosphorylation: identity of the RT-97 phosphoepitope and regulation in neurons by cross-talk among proline-directed kinases.
    J Neurochem. 2008 Oct;107(1): 35-49.
  7. Logan, A. et al. (2006) Neurotrophic factor synergy is required for neuronal survival and disinhibited axon regeneration after CNS injury.
    Brain. 129: 490-502.
  8. Lorber, B. et al. (2004) Stimulated regeneration of the crushed adult rat optic nerve correlates with attenuated expression of the protein tyrosine phosphatases RPTPalpha, STEP, and LAR.
    Mol Cell Neurosci. 27: 404-16.
  9. Reynolds, J. et al. (2005) Age-dependent changes in Fibroblast growth factor 2 (FGF-2) expression in mouse cerebellar neurons.
    J Cell Mol Med. 9: 398-406.
  10. Shin, D.H. et al. (2003) The correspondence between the labeling patterns of antibody RT97, neurofilaments, microtubule associated protein 1B and tau varies with cell types and development stages of chicken retina.
    Neurosci Lett. 342: 167-70.
  11. Connolly ,A.A. et al. (1987) A comparative study of a silver stain and monoclonal antibody reactions on Alzheimer's neurofibrillary tangles.
    J Neurol Neurosurg Psychiatry. 50: 1221-4.
  12. Doering, L.C. (1991) Transplantation of fetal CNS tissue into the peripheral nervous system: a model to study aberrant changes in the neuronal cytoskeleton.
    J Neural Transplant Plast. 2: 193-205.
  13. McCarthy, P.W. et al. (1992) RT97- and calcitonin gene-related peptide-like immunoreactivity in lumbar intervertebral discs and adjacent tissue from the rat.
    J Anat. 180: 15-24.
  14. Murphy, A. et al. (1993) Neurofilament expression in human T lymphocytes.
    Immunology. 79: 167-70.
  15. Yabe, J.T. et al. (2001) Neurofilaments consist of distinct populations that can be distinguished by C-terminal phosphorylation, bundling, and axonal transport rate in growing axonal neurites.
    J Neurosci. 21: 2195-205.
  16. Wang, S. et al. (2000) Progressive optic axon dystrophy and vacuslar changes in rd mice.
    Invest Ophthalmol Vis Sci. 41: 537-45.
  17. Kuwamura, M. et al. (2004) Cerebral ganglioneuroblastoma in a golden retriever dog.
    Vet Pathol. 41: 282-4.
  18. Rovere, G. et al. (2015) Comparison of Retinal Nerve Fiber Layer Thinning and Retinal Ganglion Cell Loss After Optic Nerve Transection in Adult Albino Rats.
    Invest Ophthalmol Vis Sci. 56 (8): 4487-98.
  19. Wang, J. et al. (2017) MicroRNA regulation in an animal model of acute ocular hypertension.
    Acta Ophthalmol. 95 (1): e10-e21.
  20. Vidal-Sanz, M. et al. (2015) Retinal neurodegeneration in experimental glaucoma.
    Prog Brain Res. 220: 1-35.
  21. Moutal, A. et al. (2020) Differential expression of Cdk5-phosphorylated CRMP2 following a spared nerve injury.
    Mol Brain. 13 (1): 97.

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