Nestin antibody | Rat-401 (4D4)

Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- TRIS buffered glycine.
- Preservative Stabilisers
- 0.05% Sodium Azide (NaN3)
- Immunogen
- Nestin purified from embryonic rat spinal cord.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P21263
- Entrez Gene
- Nes
- GO Terms
- GO:0005882 intermediate filament
- GO:0005198 structural molecule activity
- GO:0007420 brain development
- GO:0042493 response to drug
- GO:0010212 response to ionizing radiation
- GO:0019215 intermediate filament binding
- GO:0030182 neuron differentiation
- GO:0030844 positive regulation of intermediate filament depolymerization
- GO:0031076 embryonic camera-type eye development
- GO:0031667 response to nutrient levels
- GO:0031730 CCR5 chemokine receptor binding
- GO:0043066 negative regulation of apoptosis
- GO:2000179 positive regulation of neural precursor cell proliferation
- Regulatory
- For research purposes only
Applications of Nestin antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunofluorescence | |||
Immunohistology - Frozen | 1/40 | 1/400 | |
Immunohistology - Paraffin | 1/40 | 1/400 | |
Western Blotting |
- Immunohistology
- We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM. For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.
- Western Blotting
- Mouse anti Rat Nestin antibody, clone Rat-401 reacts with a band at 200-220 kDa in reducing gels of newborn rat or mouse cell extracts. For western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested.
Secondary Antibodies Available
Product Specific References
Source Reference
-
Hockfield, S. & McKay, R.D. (1985) Identification of major cell classes in the developing mammalian nervous system.
J Neurosci. 5 (12): 3310-28.
References for Nestin antibody
-
Arnold, T.D. et al. (2012) Defective Retinal Vascular Endothelial Cell Development As a Consequence of Impaired Integrin αVβ8-Mediated Activation of Transforming Growth Factor-β.
J Neurosci. 32: 1197-206. -
Mori, T. et al. (2005) Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.
Mol Cell Biol. 25: 5183-95. -
Choi, J.S. et al. (2010) Expression of vascular endothelial growth factor receptor-3 mRNA in the rat developing forebrain and retina.
J Comp Neurol. 518: 1064-81. -
Choi, J.S. et al. (2007) Upregulation of vascular endothelial growth factor receptors Flt-1 and Flk-1 following acute spinal cord contusion in rats.
J Histochem Cytochem. 55: 821-30. -
Choi, J.S. et al. (2009) Transient expression of Bis protein in midline radial glia in developing rat brainstem and spinal cord.
Cell Tissue Res. 337: 27-36. -
Shin, Y.J. et al. (2010) Enhanced expression of vascular endothelial growth factor receptor-3 in the subventricular zone of stroke-lesioned rats.
Neurosci Lett. 469: 194-8. -
Zhang, H. et al. (2003) VEGF is a chemoattractant for FGF-2-stimulated neural progenitors.
J Cell Biol. 163: 1375-84. -
Aleksandrova, M.A. et al. (2001) Transplantation of Cultured Human Neural Progenitor Cells into Rat Brain: Migration and Differentiation
Bull Exp Biol Med. 132: 1000-3. -
Bertelli, E. et al. (2002) Nestin expression in rat adrenal gland.
Histochem Cell Biol. 117: 371-7. -
Choi, J.S. et al. (2009) Enhanced expression of SOCS-2 in the rat hippocampus after transient forebrain ischemia.
J Neurotrauma. 26: 2097-106. -
Poltavtseva, R.A. et al. (2001) In vitro development of neural progenitor cells from human embryos.
Bull Exp Biol Med. 132: 861-3. -
Shin, Y.J. et al. (2013) Induction of vascular endothelial growth factor receptor-3 expression in perivascular cells of the ischemic core following focal cerebral ischemia in rats.
Acta Histochem. 115 (2): 170-7. -
Barreira, A.L. et al. (2009) Bone marrow mononuclear cells attenuate interstitial fibrosis and stimulate the repair of tubular epithelial cells after unilateral ureteral obstruction.
Cell Physiol Biochem. 24: 585-94. -
Araujo, M. R. et al. (2016) Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats
<a rel="nofollow" href="http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21596"target="_blank">Semina: Ciências Agrárias. 37 (3): 1355</a>. -
Shin, Y.J. et al. (2016) Increased expression of suppressor of cytokine signaling 2 in the subventricular zone after transient focal cerebral ischemia in adult rats.
Brain Res. Jul 26. [Epub ahead of print]
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