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Nestin antibody | Rat-401 (4D4)

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Mouse anti Rat Nestin

Product Type
Monoclonal Antibody
Clone
Rat-401 (4D4)
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
6625-1010
Datasheet
C IF P WB
SDS
0.1 mg loader

Mouse anti Rat Nestin antibody, clone Rat-401 recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glian cells (Hockfield and McKay 1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
TRIS buffered glycine.
Preservative Stabilisers
0.05% Sodium Azide (NaN3)
Immunogen
Nestin purified from embryonic rat spinal cord.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P21263
Entrez Gene
Nes
GO Terms
GO:0005882 intermediate filament
GO:0005198 structural molecule activity
GO:0007420 brain development
GO:0042493 response to drug
GO:0010212 response to ionizing radiation
GO:0019215 intermediate filament binding
GO:0030182 neuron differentiation
GO:0030844 positive regulation of intermediate filament depolymerization
GO:0031076 embryonic camera-type eye development
GO:0031667 response to nutrient levels
GO:0031730 CCR5 chemokine receptor binding
GO:0043066 negative regulation of apoptosis
GO:2000179 positive regulation of neural precursor cell proliferation
Regulatory
For research purposes only

Applications of Nestin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen 1/40 1/400
Immunohistology - Paraffin 1/40 1/400
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM. For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.
Western Blotting
Mouse anti Rat Nestin antibody, clone Rat-401 reacts with a band at 200-220 kDa in reducing gels of newborn rat or mouse cell extracts. For western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Immunofluorescence

Product Specific References

Source Reference

  1. Hockfield, S. & McKay, R.D. (1985) Identification of major cell classes in the developing mammalian nervous system.
    J Neurosci. 5 (12): 3310-28.

References for Nestin antibody

  1. Arnold, T.D. et al. (2012) Defective Retinal Vascular Endothelial Cell Development As a Consequence of Impaired Integrin αVβ8-Mediated Activation of Transforming Growth Factor-β.
    J Neurosci. 32: 1197-206.
  2. Mori, T. et al. (2005) Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.
    Mol Cell Biol. 25: 5183-95.
  3. Choi, J.S. et al. (2010) Expression of vascular endothelial growth factor receptor-3 mRNA in the rat developing forebrain and retina.
    J Comp Neurol. 518: 1064-81.
  4. Choi, J.S. et al. (2007) Upregulation of vascular endothelial growth factor receptors Flt-1 and Flk-1 following acute spinal cord contusion in rats.
    J Histochem Cytochem. 55: 821-30.
  5. Choi, J.S. et al. (2009) Transient expression of Bis protein in midline radial glia in developing rat brainstem and spinal cord.
    Cell Tissue Res. 337: 27-36.
  6. Shin, Y.J. et al. (2010) Enhanced expression of vascular endothelial growth factor receptor-3 in the subventricular zone of stroke-lesioned rats.
    Neurosci Lett. 469: 194-8.
  7. Zhang, H. et al. (2003) VEGF is a chemoattractant for FGF-2-stimulated neural progenitors.
    J Cell Biol. 163: 1375-84.
  8. Aleksandrova, M.A. et al. (2001) Transplantation of Cultured Human Neural Progenitor Cells into Rat Brain: Migration and Differentiation
    Bull Exp Biol Med. 132: 1000-3.
  9. Bertelli, E. et al. (2002) Nestin expression in rat adrenal gland.
    Histochem Cell Biol. 117: 371-7.
  10. Choi, J.S. et al. (2009) Enhanced expression of SOCS-2 in the rat hippocampus after transient forebrain ischemia.
    J Neurotrauma. 26: 2097-106.
  11. Poltavtseva, R.A. et al. (2001) In vitro development of neural progenitor cells from human embryos.
    Bull Exp Biol Med. 132: 861-3.
  12. Shin, Y.J. et al. (2013) Induction of vascular endothelial growth factor receptor-3 expression in perivascular cells of the ischemic core following focal cerebral ischemia in rats.
    Acta Histochem. 115 (2): 170-7.
  13. Barreira, A.L. et al. (2009) Bone marrow mononuclear cells attenuate interstitial fibrosis and stimulate the repair of tubular epithelial cells after unilateral ureteral obstruction.
    Cell Physiol Biochem. 24: 585-94.
  14. Araujo, M. R. et al. (2016) Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats
    <a rel="nofollow" href="http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21596"target="_blank">Semina: Ci&ecirc;ncias Agr&aacute;rias. 37 (3): 1355</a>.
  15. Shin, Y.J. et al. (2016) Increased expression of suppressor of cytokine signaling 2 in the subventricular zone after transient focal cerebral ischemia in adult rats.
    Brain Res. Jul 26. [Epub ahead of print]

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