Mouse anti Rat Nestin antibody, clone Rat-401
recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glian cells (Hockfield and McKay 1985
). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- TRIS buffered glycine.
- Preservative Stabilisers
- 0.05% Sodium Azide (NaN3)
- Nestin purified from embryonic rat spinal cord.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
structural molecule activity
response to drug
response to ionizing radiation
intermediate filament binding
positive regulation of intermediate filament depolymerization
embryonic camera-type eye development
response to nutrient levels
CCR5 chemokine receptor binding
negative regulation of apoptosis
positive regulation of neural precursor cell proliferation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Nestin antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM.
For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.
- Western Blotting
- Mouse anti Rat Nestin antibody, clone Rat-401 reacts with a band at 200-220 kDa in reducing gels of newborn rat or mouse cell extracts.
For western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Application Based External Images
Product Specific References
Hockfield, S. & McKay, R.D. (1985) Identification of major cell classes in the developing mammalian nervous system.
J Neurosci. 5 (12): 3310-28.
References for Nestin antibody
Arnold, T.D. et al. (2012) Defective Retinal Vascular Endothelial Cell Development As a Consequence of Impaired Integrin αVβ8-Mediated Activation of Transforming Growth Factor-β.
J Neurosci. 32: 1197-206.
Mori, T. et al. (2005) Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.
Mol Cell Biol. 25: 5183-95.
Choi, J.S. et al. (2010) Expression of vascular endothelial growth factor receptor-3 mRNA in the rat developing forebrain and retina.
J Comp Neurol. 518: 1064-81.
Choi, J.S. et al. (2007) Upregulation of vascular endothelial growth factor receptors Flt-1 and Flk-1 following acute spinal cord contusion in rats.
J Histochem Cytochem. 55: 821-30.
Choi, J.S. et al. (2009) Transient expression of Bis protein in midline radial glia in developing rat brainstem and spinal cord.
Cell Tissue Res. 337: 27-36.
Shin, Y.J. et al. (2010) Enhanced expression of vascular endothelial growth factor receptor-3 in the subventricular zone of stroke-lesioned rats.
Neurosci Lett. 469: 194-8.
Zhang, H. et al. (2003) VEGF is a chemoattractant for FGF-2-stimulated neural progenitors.
J Cell Biol. 163: 1375-84.
Aleksandrova, M.A. et al. (2001) Transplantation of Cultured Human Neural Progenitor Cells into Rat Brain: Migration and Differentiation
Bull Exp Biol Med. 132: 1000-3.
Bertelli, E. et al. (2002) Nestin expression in rat adrenal gland.
Histochem Cell Biol. 117: 371-7.
Choi, J.S. et al. (2009) Enhanced expression of SOCS-2 in the rat hippocampus after transient forebrain ischemia.
J Neurotrauma. 26: 2097-106.
Poltavtseva, R.A. et al. (2001) In vitro development of neural progenitor cells from human embryos.
Bull Exp Biol Med. 132: 861-3.
Shin, Y.J. et al. (2013) Induction of vascular endothelial growth factor receptor-3 expression in perivascular cells of the ischemic core following focal cerebral ischemia in rats.
Acta Histochem. 115 (2): 170-7.
Barreira, A.L. et al. (2009) Bone marrow mononuclear cells attenuate interstitial fibrosis and stimulate the repair of tubular epithelial cells after unilateral ureteral obstruction.
Cell Physiol Biochem. 24: 585-94.
Araujo, M. R. et al. (2016) Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats
<a rel="nofollow" href="http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21596"target="_blank">Semina: Ciências Agrárias. 37 (3): 1355</a>.
Shin, Y.J. et al. (2016) Increased expression of suppressor of cytokine signaling 2 in the subventricular zone after transient focal cerebral ischemia in adult rats.
Brain Res. Jul 26. [Epub ahead of print]
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