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MHC Class II RT1Bu/L antibody | OX-3

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Anti Rat MHC Class II RT1Bu/L Antibody, clone OX-3 gallery image 1
Published customer image:
Mouse anti Rat MHC class II antibody, clone OX-3 (MCA45R) used to label MHC class II expressing cells in formalin fixed mouse brain cryosections by immunohistochemistry.
Image caption:
(a) Representative immunostained micrographs show Iba1+ cells in the SN of 3-, 6-, 9-, and 12-month-old female and male mice. Scale bar: 10 μm. Quantitative results of Iba1+ cell areas (b) and the number of Iba1+ cells (c) in the SN of female and male mice at different ages (n = 5). *(p <0.05), **(p <0.01), ***(p <0.001): versus the opposite sex. (d) Representative immunostained micrographs show MHC class II+ cells in the SN of 3-, 6-, 9-, and 12-month-old female and male mice. Scale bar: 10 μm. Quantitative results of MHC class II+ cell areas (e) and the number of MHC class II+ cells (f) in the SN of female and male mice at different ages (n = 5). ***(p <0.001): versus the opposite sex. (g) Representative immunostained micrographs show TH+ cells in the SN of 3-, 6-, 9-, and 12-month-old female and male mice. Scale bar: 200 μm. (h) Quantitative results of TH+ cells in the SN of female and male mice at different ages (n = 5). *(p <0.05), **(p <0.01): versus the opposite sex.

From: Wu SY, Chen YW, Tsai SF, Wu SN, Shih YH, Jiang-Shieh YF, Yang TT, Kuo YM.
Estrogen ameliorates microglial activation by inhibiting the Kir2.1 inward-rectifier K(+) channel.
Sci Rep. 2016 Mar 10;6:22864.

Mouse anti Rat MHC Class II RT1Bu/L:FITC

Mouse anti Rat MHC Class II RT1Bu/L

Mouse anti Rat MHC Class II RT1Bu/L:RPE

Product Type
Monoclonal Antibody
Clone
OX-3
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA45FT
Datasheet
F
MSDS
0.1 mg loader

MCA45GA
Datasheet
C F P
MSDS
0.1 mg loader

MCA45R
Datasheet
C F P
MSDS
0.25 mg loader

MCA45PE
Datasheet
F
MSDS
100 Tests loader

Mouse anti Rat MHC Class II RT1Bu/L antibody, clone OX-3 recognizes a polymorphic determinant of the rat RT1B MHC class II antigen, reacting with haplotypes u and l. The literature reports reactivity with Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. This antibody is useful for distinguishing RT1B positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.

Mouse anti Rat MHC Class II RT1Bu/L antibody, clone OX-3 also cross reacts with mouse strains of the H-2 haplotypes b and s. Analysis of recombinant mouse strains has mapped the OX-3 determinant to the H-2I-A region.

This product is routinely tested in flow cytometry on Lewis rat splenocytes.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
Pack Size: 0.1 mg
0.09%Sodium Azide
Pack Size: 0.25 mg
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Rat thymocyte membrane glycoproteins.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 0.1 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.25 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch
Guarantee
6 months from date of reconstitution

More Information

Regulatory
For research purposes only

Applications of MHC Class II RT1Bu/L antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/100
Immunohistology - Frozen
Immunohistology - Paraffin
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining.

This product does not require antigen retrieval using heat treatment methods prior to staining of paraffin sections.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
Mouse IgG1 Negative Control:RPE MCA1209PE F 100 Tests loader

Application Based External Images

Immunohistology - Frozen

Product Specific References

References for MHC Class II RT1Bu/L antibody

  1. McMaster, W.R. & Williams, A.F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.
    Eur J Immunol. 9 (6): 426-33.
  2. McMaster, W.R. & Williams, A.F. (1979) Monoclonal antibodies to Ia antigens from rat thymus: cross reactions with mouse and human and use in purification of rat Ia glycoproteins.
    Immunol Rev. 47: 117-37.
  3. Barclay, A.N. & Mayrhofer, G. (1981) Bone marrow origin of Ia-positive cells in the medulla rat thymus.
    J Exp Med. 153 (6): 1666-71.
  4. Zhang, J. et al. (1997) Expression of major histocompatibility complex molecules in rodent retina. Immunohistochemical study.
    Invest Ophthalmol Vis Sci. 38 (9): 1848-57.
  5. Hahm, K.B. et al. (2000) Loss of TGF-beta signaling contributes to autoimmune pancreatitis.
    J Clin Invest. 105 (8): 1057-65.
  6. Wu, S.Y. et al. (2016) Estrogen ameliorates microglial activation by inhibiting the Kir2.1 inward-rectifier K(+) channel.
    Sci Rep. 6: 22864.
  7. Fisher, R.A. et al. (1996) Induction of long-term graft tolerance and donor/recipient chimerism.
    J Surg Res. 60 (1): 181-5.
  8. Keller, R. et al. (1988) Modulation of major histocompatibility complex (MHC) expression by interferons and microbial agents. Independent regulation of MHC class II expression and induction of tumoricidal activity in bone marrow-derived mononuclear phagocytes.
    Scand J Immunol. 28 (1): 113-21.
  9. Streit, W.J. et al. (1989) Peripheral nerve lesion produces increased levels of major histocompatibility complex antigens in the central nervous system.
    J Neuroimmunol. 21 (2-3): 117-23.
  10. Roggin, K.K. et al. (2001) Macrophage phenotype during cholestatic injury and repair: the persistent inflammatory response.
    J Pediatr Surg. 36 (1): 220-8.
  11. Reutzel-Selke A et al. (2003) Short-term immunosuppressive treatment of the donor ameliorates consequences of ischemia/ reperfusion injury and long-term graft function in renal allografts from older donors.
    Transplantation. 75 (11): 1786-92.
  12. Heidenhain, C. et al. (2003) The impact of immune-activating processes following transplantation on chronic allograft nephropathy.
    Kidney Int. 64 (3): 1125-33.
  13. Hahm, K.B. et al. (2001) Loss of transforming growth factor beta signalling in the intestine contributes to tissue injury in inflammatory bowel disease.
    Gut. 49 (2): 190-8.
  14. Pascher A et al. (2006) Rat cytomegalovirus infection interferes with anti-CD4 mAb-(RIB 5/2) mediated tolerance and induces chronic allograft damage.
    Am J Transplant. 6 (9): 2035-45.
  15. Hartmann CB et al. (2005) Immunotoxicity of gallium arsenide on antigen presentation: comparative study of intratracheal and intraperitoneal exposure routes.
    J Immunotoxicol. 2 (1): 1-9.

Further Reading

  1. Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42 (4): 593-600.
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